The effects of recombinant human BMP-7, prepared from a COS-7 expression system, on the proliferation and differentiation of rat newborn calvarial osteoblasts

Yonsei Medical Journal

Volumn 44, Issue 4, 2003, Pages 593-601

  Lee, Dong Hee ^a   Suh, Hwal ^a   Han, Dong Wook ^a   Park, Bong Joo ^a   Lee, Jin Woo ^a   Park, Jong Chul ^a  

^a Yonsei University College of Medicine   (South Korea)

Author keywords

Alkaline phosphatase; Bone morphologenetic protein; cAMP; COS 7; DNA synthesis; Osteoblast; Osteocalcin; Rat newborn calvaria cell

Indexed keywords

ALKALINE PHOSPHATASE; BONE MORPHOGENETIC PROTEIN; CYCLIC AMP; DISULFIDE; OSTEOCALCIN; OSTEOGENIC PROTEIN 1; PARATHYROID HORMONE;

ANIMAL CELL; ARTICLE; BONE MINERALIZATION; CALVARIA; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; CELL STRAIN COS7; CHO CELL; CHONDROGENESIS; CONTROLLED STUDY; ENZYME INDUCTION; HOST CELL; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR WEIGHT; NEWBORN; NONHUMAN; OSSIFICATION; OSTEOBLAST; PHENOTYPE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN SYNTHESIS; RAT;

EID: 0042882117     PISSN: 05135796     EISSN: None     Source Type: Journal    
DOI: 10.3349/ymj.2003.44.4.593     Document Type: Article

References (30)

1. Reddi AH. Bone and cartilage differentiation. Curr Opin Genet Dev 1994;4:737-44.
2. Rosen V, Thies RS. The BMP proteins in bone formation and repair. Trends Genet 1992;8:97-102.
3. Wozney JM. The bone morphogenetic protein family and osteogenesis. Mol Reprod Dev 1992;32:160-7.
4. Wozney JM, Rosen V, Celeste AJ, Mitsock LM, Whitters MJ, Kriz RW, et al. Novel regulators of bone formation: molecular clones and activities. Science 1988;242:1528-34.
5. Celeste AJ, Iannazzi JA, Taylor RC, Hewick RM, Rosen V, Wang EA, et al. Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone. Proc Natl Aca Sci USA 1990;87:9843-7.
6. Ozkaynak E, Rueger DC, Drier EA, Corbett C, Ridge RJ, Sampath TK, et al. OP-1 cDNA encodes an osteogenic protein in the TGF-beta family. EMBO J 1990; 9:2085-93.
7. Ozkaynak E, Schnegelsberg PN, Jin DF, Clifford GM, Warren FD, Drier EA, et al. Osteogenic protein-2. A new member of the transforming growth factor-beta superfamily expressed early in embryogenesis. J Biol Chem 1992;267:25220-7.
8. Celeste AJ, Song JJ, Cox K, Rosen V, Wozney JM. Bone morphogenetic protein-9, a new member of the TGF beta superfamily. J Bone Miner Res 1994;9:136.
9. Celeste AJ, Ross JL, Yamaji N, Wozney JM. The molecular cloning of human bone morphogenetic proteins-10, -11 and -12, three new members of the transforming growth factor-superfamily. J Bone Miner Res 1995;10:S336.
10. Storm EE, Huynh TV, Copeland NG, Jenkins NA, Kingsley DM, Lee SJ. Limb alterations in brachypodism mice due to mutations in a new member of the TGF-beta superfamily. Nature 1994;368:639-43.
11. Dube JL, Celeste AJ. Human bone morphogenetic protein-15, a new member of the transforming growth factor beta superfamilyy. J Bone Miner Res 1996;11:S382.
12. Kingsley DM. The TGF-beta superfamily: new members, new receptors, and new genetic tests of function in different organisms. Genes Dev 1994;8:133-46.
13. Sampath TK, Maliakal JC, Hauschka. PV, Jones WK, Sasak H, Tucker RF, et al. Recombinant human osteogenic protein-1 (hOP-1) induces new bone formation in vivo with a specific activity comparable with natural bovine osteogenic protein and stimulates osteoblast proliferation and differentiation in vitro. J Biol Chem 1992;267:20352-62.
14. Wang EA, Rosen V, D'Alessandro JS, Bauduy M, Cordes P, Harada T, et al. Recombinant human bone morphogenetic protein induces bone formation. Proc Natl Acad Sci USA 1990;87:2220-4.
15. Yokose S, Ishizuya T, Ikeda T, Nakamura T, Tsurukami H, Kawasaki K, et al. An estrogen deficiency caused by ovariectomy increases plasma levels of systemic factors that stimulate proliferation and differentiation of osteoblasts in rats. Endocrinology 1996;137:469-78.
16. Sampath TK, Coughlin JE, Whetstone RM, Banach D, Corbett C, et al. Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-beta superfamily. J Biol Chem 1990;265:13198-205.
17. Vytasek R. A sensitive fluorometric assay for the determination of DNA. Anal Biochem 1982;120:243-8.
18. Vukicevic S, Luyten FP, Reddi AH. Stimulation of the expression of osteogenic and chondrogenic phenotypes in vitro by osteogenin. Proc Natl Acad Sci USA 1989; 86:8793-7.
19. Vukicevic S, Luyten FP, Reddi AH. Osteogenin inhibits proliferation and stimulates differentiation in mouse osteoblast-like cells (MC3T3-E1). Biochem Biophys Res Commun 1990;166:750-6.
20. Katagiri T, Yamaguchi A, Ikeda T, Yoshiki S, Wozney JM, Rosen V, et al. The non-osteogenic mouse pluripotent cell line, C3H1OT1/2, is induced to differentiate into osteoblastic cells by recombinant human bone morphogenetic protein-2. Biochem Biophys Res Commun 1990;172:295-9.
21. Takuwa Y, Ohse C, Wang EA, Wozney JM, Yamashita K. Bone morphogenetic protein-2 stimulates alkaline phosphatase activity and collagen synthesis in cultured osteoblastic cells, MC3T3-E. Biochem Biophys Res Commun 1991;174:96-101.
22. Yamaguchi A, Katagiri T, Ikeda T, Wozney JM, Rosen V, Wang EA, et al. Recombinant human bone morphogenetic protein-2 stimulates osteoblastic maturation and inhibits myogenic differentiation in vitro. J Cell Biol 1991;113:681-7.
23. Chen TL, Bates RL, Dudley A, Hammonds RG Jr, Amento EP. Bone morphogenetic protein-2b stimulation of growth and osteogenic phenotypes in rat osteoblast-like cells: comparison with TGF-beta 1. J Bone Miner Res 1991;6:1387-93.
24. Wong GL, Cohn DV. Target cells in bone for parathormone and calcitonin are different: enrichment for each cell type by sequential digestion of mouse calvaria and selective adhesion to polymeric surfaces. Proc Natl Acad Sci USA 1975;72:3167-71.
25. Aronow MA, Gerstenfeld LC, Owen TA, Tassinari MS, Stein GS, Lian JB. Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells. J Cell Physiol 1990;143:213-21.
26. Auf'mkolk B, Hauschka PV, Schwartz ER. Characterization of human bone cells in culture. Calcif Tissue Int 1985;37:228-35.
27. Jones WK, Richmond EA, White K, Sasak H, Kusmik W, Smart J, et al. Osteogenic protein-1 (OP-1) expression and processing in Chinese hamster ovary cells: isolation of a-soluble complex containing the mature and pro-domains of OP-1. Growth Factors 1994;11:215-25.
28. Hazama M, Aono A, Ueno N, Fujisawa Y. Efficient expression of a heterodimer of bone morphogenetic protein subunits using a baculovirus expression system. Biochem Biophys Res Common 1995;209:859-66.
29. Asahina I, Sampath TK, Nishimura I, Hauschka PV. Human osteogenic protein-1 induces both chondroblastic and osteoblastic differentiation of osteoprogenitor cells derived from newborn rat calvaria. J Cell Biol 1993;123:921-33.
30. Maliakal JC, Asahina I, Hauschka PV, Sampath TK. Osteogenic protein-1 (BMP-7) inhibits cell proliferation and stimulates the expression of markers characteristic of osteoblast phenotype in rat osteosarcoma (17/2.8) cells. Growth Factors 1994;11:227-34.