Purification and characterization of a novel anticoagulant and fibrinolytic enzyme produced by endophytic bacterium Paenibacillus polymyxa EJS-3

Thrombosis Research

Volumn 126, Issue 5, 2010, Pages

  Lu, Fengxia ^a   Lu, Zhaoxin ^a   Bie, Xiaomei ^a   Yao, Zhengying ^a   Wang, Yufeng ^a   Lu, Yaping ^a   Guo, Yao ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

Endophytes; Fibrinolytic activity; Fibrinolytic enzyme; Paenibacillus polymyxa

Indexed keywords

AMMONIUM SULFATE; ANTICOAGULANT AGENT; CALCIUM; CHYMOTRYPSIN; COPPER; FERROUS ION; MAGNESIUM; PAENIBACILLUS POLYMYXA FIBRINOLYTIC ENZYME; UNCLASSIFIED DRUG; ZINC;

ARTICLE; BETA CHAIN; ENDOPHYTE; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; FIBRINOLYSIS; GEL FILTRATION CHROMATOGRAPHY; HYDROLYSIS; ION EXCHANGE CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PAENIBACILLUS POLYMYXA; PRECIPITATION; PRIORITY JOURNAL; THROMBOSIS;

ANTICOAGULANTS; ENZYME STABILITY; FIBRIN; FIBRIN MODULATING AGENTS; FIBRINOLYSIS; FIBRINOLYTIC AGENTS; HUMANS; HYDROGEN-ION CONCENTRATION; PAENIBACILLUS; PROTEASE INHIBITORS;

EID: 78149285759     PISSN: 00493848     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.thromres.2010.08.003     Document Type: Article

References (38)

1. K. Ouriel Current status of thrombolysis for peripheral arterial occlusive disease Ann Vasc Surg 16 2002 797 840
2. D. Collen, and H.R. Lijnen Tissue-type plasminogen activator: a historical perspective and personal account J Thromb Haemost 2 2004 541 546
3. M.J. Duffy Urokinase plasminogen activator and its inhibitor, PAI-1, as prognostic markers in breast cancer: from pilot to level 1 evidence studies Clin Chem 48 2002 1194 1197
4. H. Sumi, H. Hamada, H. Tsushima, H. Mihara, and H. Muraki A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto; a typical and popular soybean food in the Japanese diet Experientia 43 1987 1110 1111
5. H. Mihara, H. Sumi, T. Yoneta, H. Mizumoto, R. Ikeda, and M. Seiki A novel fibrinolytic enzyme extracted from the earthworm, Lumbricus rubellus Jpn J Physiol 41 1991 461 472
6. Y. Zhang, A. Wisner, Y.L. Xiong, and C. Bon A novel plasminogen activator from snake venom J Biol Chem 270 1995 10246 10255
7. M.Y. Ahn, B.S. Hahn, K.S. Ryu, J.W. Kim, I. Kim, and Y.S. Kim Purification and characterization of a serine protease with fibrinolytic activity from the dung beetles, Catharsius molossus Thromb Res 112 2003 339 347
8. H.R. Maurer Bromelain: biochemistry, pharmacology and medical use Cell Mol Life Sci 58 2001 1234 1245
9. M. Fujita, K. Nomura, K. Hong, Y. Ito, A. Asada, and S. Nishimuro Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in vegetable cheese natto, a popular soybean fermented food in Japan Biochem Biophys Res Commun 197 1993 1340 1347
10. W. Kim, K. Choi, Y. Kim, H. Park, J. Choi, and Y. Lee Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang Appl Environ Microbiol 62 1996 2482 2488
11. R.R. Chitte, and S. Dey Potent fibrinolytic enzyme from a thermophilic Streptomyces megasporus strain SD5 Lett Appl Microbiol 31 2000 405 410
12. Y. Peng, Q. Huang, R.H. Zhang, and Y.Z. Zhang Purification and characterization of a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4 screened from douchi, a traditional Chinese soybean food Comp Biochem Physiol Biochem Mol Biol 134 2003 45 52
13. Y. Peng, X. Yang, and Y. Zhang Microbial fibrinolytic enzymes: an overview of source, production, properties, and thrombolytic activity in vivo Appl Microbiol Biotechnol 69 2005 126 132
14. J. Ko, J. Yan, L. Zhu, and Y. Qi Subtilisin QK, a fibrinolytic enzyme, inhibits the exogenous nitrite and hydrogen peroxide induced protein nitration, in vitro and in vivo J Biochem Mol Biol 38 2005 577 583
15. G.A. Strobel Endophytes as sources of bioactive products Microbiol Infect 5 2003 535 544
16. B. Guo, Y. Wang, X. Sun, and K. Tang Bioactive natural products from endophytes: A review Appl Biochem Microbiol 44 2008 136 142
17. Y. Li, J.L. Shuang, W.W. Yuan, W.Y. Huang, and R.X. Tan Verticase: a Fibrinolytic Enzyme Produced by Verticillium sp. Tj33, an Endophyte of Trachelospermum jasminoides J Integrat Plant Biol 49 2007 1548 1554
18. U. Mitsuhiro, K. Toshihiro, M. Kazutaka, and N. Takumi Purification and characterization of fibrinolytic alkaline protease from Fusarium sp. BLB. Appl Microbiol Biotechnol 74 2007 331 338
19. B. Wu, L.C. Wu, D.J. Chen, Z.J. Yang, and M.Y. Luo Purification and characterization of a novel fibrinolytic protease from Fusarium sp. CPCC 480097 J Ind Microbiol Biotechnol 36 2009 451 459
20. F.X. Lu, L.J. Sun, Z.X. Lu, X.M. Bie, Y.W. Fang, and S. Liu Isolation and Identification of an endophytic Strain EJS-3 producing novel fibrinolytic enzymes Curr Microbiol 54 2007 435 439
21. T. Astrup, and S. Mullertz The fibrin plate method for estimating fibrinolytic activity Arch Biochem Biophys 40 1952 346 351
22. M.M. Bradford A rapid and sensitive method for the quantitation of microgramquantities of protein utilizing the principle of protein-dry binding Anal Biochem 72 1976 248 254
23. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
24. N.S. Choi, and S.H. Kim Application of fibrin zymography for determining the optimum culture time for protease activity Biotechnol Tech 13 1999 899 901
25. N.S. Choi, and S.H. Kim Two fibrin zymography methods for analysis of plasminogen activators on gels Anal Biochem 281 2000 236 238
26. S.H. Kim Choi NS, Lee WY. Fibrin zymography: A direct analysis of fibrinolytic enzymes on gels Anal Biochem 263 1998 115 116
27. M. Kussmann, E. Nordhoff, H. Rahbek-Nielsen, S. Haebel, M. Rossel-Larsen, and L. Jakobsen Matrix-assissted laser desorption/ionization mass spectrometry sample preparation techiques designed for various peptide and protein Analytes J Mass Spectrom 32 1997 593 601
28. Y.S. Koh, K.H. Chung, and D.S. Kim Biochemical characterization of a thrombin-like enzyme from snake Toxcion 39 2001 555 560
29. J.S. Kim, S. Kumar, S.E. Park, B.S. Choi, S. Kim, and T.H. Nguyen A fibrinolytic enzyme from the medicinal mushroom Cordyceps militaris J Microbiol 44 2006 622 631
30. S.K. Lee, D.H. Bae, T.J. Kwon, S.B. Lee, H.H. Lee, and J.H. Park Purification and characterization of a fibrinolytic enzyme from Bacillus sp. KDO-13 isolated from soybean paste J Microbiol Biotechnol 11 2001 845 852
31. H.D. Paik, S.K. Lee, S. Heo, S.Y. Kim, H. Lee, and T.J. Kwon Purification and characterization of the fibrinolytic enzyme produced by Bacillus subtilis KCK-7 from Chungkookjang J Microbiol Biotechnol 14 2004 829 835
32. H.K. Kim, G.T. Kim, D.K. Kim, W.A. Choi, S.H. Park, and Y.K. Jeong Purification and characterization of a novel fibrinolytic enzyme from Bacillus sp. KA38 originated from fermented fish J Ferment Bioeng 84 1997 307 312
33. S.Y. Lee, J.S. Kim, J.E. Kim, K. Sapkota, M.H. Shen, and S. Kim Purification and characterization of fibrinolytic enzyme from cultured mycelia of Armillaria mellea Protein Expr Purif 43 2005 10 17
34. J.R. Simkhada, P. Mander, S.S. Cho, and J.C. Yoo A novel fibrinolytic protease from Streptomyces sp. CS684 Process Biochem 45 2010 88 93
35. S.L. Wang, H.J. Chen, T.W. Liang, and Y.D. Lin A novel nattokinase produced by Pseudomonas sp. TKU015 using shrimp shells as substrate Process Biochem 44 2009 70 76
36. C.T. Wang, B.P. Ji, B. Li, N. Rob, P.L. Li, and H. Ji Purification and characterization of a fibrinolytic enzyme of Bacillus subtilis DC33, isolated from Chinese traditional Douchi J Ind Microbiol Biotechnol 33 2006 750 758
37. C.T. Chang, M.H. Fan, F.C. Kuo, and H.Y. Sung Potent fibrinolytic enzyme from a mutant of Bacillus subtilis IMR-NK1 J Agric Food Chem 48 2000 3210 3216
38. N.S. Choi, J.J. Song, D.M. Chung, Y.J. Kim, P.J. Maeng, and S.H. Kim Purification and characterization of a novel thermoacid-stable fibrinolytic enzyme from Staphylococcus sp. strain AJ isolated from Korean salt-fermented Anchovy-joet J Ind Microbiol Biotechnol 36 2009 417 426