Isolation and identification of an endophytic strain EJS-3 producing novel fibrinolytic enzymes

Current Microbiology

Volumn 54, Issue 6, 2007, Pages 435-439

  Lu, Fengxia ^a   Sun, Lijun ^a   Lu, Zhaoxin ^a   Bie, Xiaomei ^a   Fang, Yaowei ^a   Liu, Shu ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

DODECYL SULFATE SODIUM; FIBRIN; PLASMIN; RNA 16S; SERINE DERIVATIVE;

ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CONTROLLED STUDY; DRUG IDENTIFICATION; DRUG SYNTHESIS; ENDOPHYTE; MOLECULAR WEIGHT; NONHUMAN; PAENIBACILLUS POLYMYXA; PRIORITY JOURNAL; STEMONA JAPONICA; STEMONACEAE; ZYMOGRAPHY;

BACILLUS; FIBRIN; FIBRINOLYTIC AGENTS; GRAM-POSITIVE ENDOSPORE-FORMING RODS; SERINE ENDOPEPTIDASES; STEMONACEAE; SUBSTRATE SPECIFICITY;

PAENIBACILLUS POLYMYXA; STEMONA JAPONICA;

EID: 34248995294     PISSN: 03438651     EISSN: 14320991     Source Type: Journal    
DOI: 10.1007/s00284-006-0591-7     Document Type: Article

References (38)

1. Astrup T, Mullertz S (1952) The fibrin plate method for estimating fibrinolytic activity. Arch Biochem Biophys 40:346-351
2. Batomunkueva BP, Egorov NS (2001) Isolation, purification and resolution of the extracellular proteinase complex of Aspergillus ochraceus 513 with fibrinolytic and anticoagulant activities. Microbiology 70(5):519-522
3. Chang CT, Fan MH, Kuo FC, Sung HY (2000) Potent fibrinolytic enzyme from amutant of Bacillus subtilis IMR-NK1. J Agric Food Chem 48(8):3210-3216
4. Chitte RR, Dey S (2000) Potent fibrinolytic enzyme from a thermophilic Streptomyces megasporus strain SD5. Lett Appl Microbiol 31(6):405-410
5. Choi NS, Kim SH (1999) Application of fibrin zymography for determining the optimum culture time for protease activity. Biotechnol Tech 13:899-901
6. Choi NS, Kim SH (2000) Two fibrin zymography methods for analysis of plasminogen activators on gels. Anal. Biochem 281:236-238
7. El-Aassar SA, El-Badry HM, Abdel-Fattah AF (1990) The biosynthesis of proteases with fibrinolytic activity in immobilized cultures of Penicillium chrysogenum H9. Appl Microbiol Biotechnol 33(1):26-30
8. Fujita M, Nomura K, Hong K, Ito Y, Asada A, Nishimuro S (1993) Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in Japan. Biochem Biophys Res Commun 197(3):1340-1347
9. Gary Strobel A (2003) Endophytes as sources of bioactive products. Microbes Infect 5(6):535-544
10. Goldhaber SZ, Bounameaux H (2001) Thrombolytic therapy in pulmonary embolism. Semin Vasc Med 1(2):213-220
11. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey's manual of determinative bacteriology, 9th ed. The Williams & Wilkins Co., Baltimore
12. Juho K (2002) Identification of a novel gene palk encoded a unique fibrinolytic enzyme larvakinase from Paenibacillus larvae QK02. Available from http://www.br.expasy.org/uniprot/Q8GBY0
13. Kim HK, Kim GT, Kim DK, Choi WA, Park SH, Jeong YK, Kong IS (1997) Purification and characterization of a novel fibrinolytic enzyme from Bacillus sp. KA38 originated from fermented fish. J. Ferment Bioeng 84(4):307-312
14. Kim SB, Lee DW, Cheigh CI (2006) Purification and characterization of a fibrinolytic subtilisin-like protease of Bacillus subtilis TP-6 from an Indonesian fermented soybean, Tempeh. J Indian Microbiol Biotechnol 33:436-444
15. Kim SH, Choi NS (2000) Purification and characterization of subtilisin DJ-4 secreted by Bacillus sp strain DJ-4 screened from Doen-Jang. Biosci Biotechnol Biochem 64:1722-1725
16. Kim SH, Choi NS, Lee WY (1998) Fibrin zymography: a direct analysis of fibrinolytic enzymes on gels. Anal Biochem 263:115-116
17. Kim SH, Choi NS (1999) Electrophoretic analysis of protease inhibitors in fibrin zymography. Anal Biochem 270:179-181
18. Kim W, Choi K, Kim Y, Park H, Choi J, Lee Y, Oh H, Kwon I, Lee S (1996) Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang. Appl Environ Microbiol 62(7):2482-2488
19. Ko JH, Yan JP, Zhu L, Qi YP (2004) Identification of two novel fibrinolytic enzymes from Bacillus subtilis QK02. Comp. Biochem Physiol C: Toxicol Pharmacol 137:65-74
20. Lee SK, Bae DH, Kwon TJ, Lee SB, Lee HH, Park JH, Heo S, Johnson MG (2001) Purification and characterization of a fibrinolytic enzyme from Bacillus sp. KDO-13 isolated from soybean paste. J Microbiol Biotechnol 11(5):845-852
21. Luk'ianchuk VV, Reva ON, Polishchuk LV (2002) Endophytic bacilli: producers of type II restriction endonucleases. Mikrobiologia 71(4):491-493
22. Manabu S, Miho T, Katsuichi S, Michiko T, Atsushi Y, Fusao T (2002) Endophytes as producers of xylanase. J Biosci Bioeng 93(1):88-90
23. Matsubara K, Sumi H, Hori K, Miyazawa K (1998) Purification and characterization of two fibrinolytic enzymes from a marine green alga, Codium intricatum. Comp Biochem Physiol Biochem Mol Biol 119:177-181
24. Matsubara K, Hori K, Matsuura Y, Miyazawa K (1999) A fibrinolytic enzyme from a marine green alga, Codium latum. Phytochemistry 52(6):993-999
25. Matsubara K, Hori K, Matsuura Y, Miyazawa K (2000) Purification and characterization of a fibrinolytic enzyme and identification of fibrinogen clotting enzyme in a marine green alga, Codium divaricatum. Comp Biochem Physiol Biochem Mol Biol 125(1):137-143
26. Medved LV, Solovjov DA, Ingham KC (1966) Domain structure, stability and interactions in streptokinase. Eur J Biochem 239:333-339
27. Nakajima N, Taya N, Sumi H (1993) Potent fibrinolytic enzyme from the lysate of Katsuwonus pelamis digestive tract (shiokara): purification and characterization. Biosci Biotechnol Biochem 57:1604-1605
28. Peng Y, Huang Q, Zhang RH, Zhang YZ (2003) Purification and characterization of a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4 screened from douchi, a traditional Chinese soybean food. Comp Biochem Physiol Biochem Mol Biol 134:45-52
29. Pennica D, Holmes WE, Kohr WJ, Harkins RN, Vehar GA,Ward CA, Bennett WF, Yelverton E, Seeburg PH, Heyne-ker HL, Goeddel DV, Collen D (1983) Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli. Nature 301:214-221
30. Sambrook J, Frisch ER, Maniatis T (1989) Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
31. 125I-labeled human high molecular weight urokinase across the intestinal tract in a dog model with stimulation of ynthesis and/or release of plasminogen activators. Blood 66:69-75
32. Schulz B, Sucker J, Aust HJ, Krohn K, Ludewig K, Jones PG, Doring D (1995) Biologically active secondary metabolites of endophytic Pezicula species. Mycol Res 99:1007-1015
33. Sumi H, Hamada H, Tsushima H, Mihara H, Muraki H (1987) A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto; a typical and popular soybean food in the Japanese diet. Experientia 43(10):110-1111
34. Sun T, Liu BH, Li P, Liu DM, Li ZH (1998) New solid-state fermentation process for repeated batch production of fibrinolytic enzyme by Fusarium oxysporum. Process Biochem 33(4):419-422
35. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence weighing, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680
36. Tough J (2005) Thrombolytic therapy in acute myocardial infarction. Nurs Stand 19(37):55-64
37. Xiao-Lan L, Lian-Xiang D, Fu-Ping L, Xi-Qun Z, Jing X (2005) Purification and characterization of a novel fibrinolytic enzyme from Rhizopus chinensis 12. Appl Microbiol Biotechnol 67(2):209-214
38. Wun D, Voet JG (1982) Isolation and characterization of urokinase from human plasma. J Biol Chem 257:3276-3283