Purification and characterization of a novel fibrinolytic enzyme from Bacillus sp. KA38 originated from fermented fish

Journal of Fermentation and Bioengineering

Volumn 84, Issue 4, 1997, Pages 307-312

  Kim, Hyun Kuk ^a   Kim, G U Taek ^a   Kim, Dae Kyung ^a   Choi, Won A ^b   Park, Sung Hoon ^b   Jeong, Yong Kee ^c   Kong, I N Soo ^d  

^a Pukyong National University   (South Korea)

^b Pusan National University   (South Korea)

^c Dong Eui University   (South Korea)

^d NONE

Author keywords

Bacillus; Fermented fish; Fibrinolytic enzyme

Indexed keywords

AMINO ACIDS; BACTERIA; BIODEGRADATION; CATALYST ACTIVITY; CATALYST SELECTIVITY; COLUMN CHROMATOGRAPHY; ELECTROPHORESIS; FERMENTATION; FRACTIONATION; MOLECULAR WEIGHT; ORGANOMETALLICS; PURIFICATION;

FIBRINOLYTIC ENZYMES; METALLOPROTEASE;

ENZYMES;

BACTERIAL ENZYME;

AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS; CONTROLLED STUDY; ELECTROPHORETIC MOBILITY; ENZYME ANALYSIS; ENZYME PURIFICATION; FERMENTATION; FIBRINOLYSIS; FISH; KOREA; MOLECULAR WEIGHT; NONHUMAN;

EID: 0031442243     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0922-338X(97)89249-5     Document Type: Article

References (24)

1. Voet, D. and Voet, J. G.: Biochemistry, p. 1087-1095. John Wiley & Sons, New York (1990).
2. Wun, T. C., Schleuning, W. D., and Reich, E.: Isolation and characterization of urokinase from human plasma. J. Biol. Chem., 257, 3276-3283 (1982).
3. Pennica, D., Holmes, W. E., Kohr, W. J., Harkins, R. N., Vehar, G. A., Ward, C. A., Bennett, W. F., Yelverton, E., Seeburg, P. H., Heyneker, H. L., Goeddel, D. V., and Collen, D.: Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli. Nature, 301, 214-221 (1983).
4. El-Aassar, S. A.: Production and properties of fibrinolytic enzyme in solid state cultures of Fusarium pallidoroseum. Biotechnol. Lett., 17, 943-948 (1995).
5. Reed, G. L., Lin, L. F., Parhami-Seren, B., and Russie, P.: Identification of plasminogen binding region in streptokinase that is necessary for the creation of functional streptokinase-plasminogen activator complex. Biochemistry, 34, 10266-10271 (1995).
6. Lijnen, H. R., van Hoef, B., de Cock, F., Okada, K., Ueshima, S., Matsuo, O., and Collen, D.: On the mechanism of fibrin-specific plasminogen activation by staphylokinase. J. Biol. Chem., 266, 11826-11832 (1991).
7. Medved, L. V., Solovjov, D. A., and Ingham, K. C.: Domain structure, stability and interactions in streptokinase. Eur. J. Biochem., 239, 333-339 (1996).
8. Arai, K., Mimuro, J., Madoiwa, S., Matsuda, M., Sako, T., and Sakata, Y.: Effect of staphylokinase concentration of plasminogen activation. Biochim. Biophys. Acta, 1245, 69-75 (1995).
9. Sumi, H., Maruyama, M., Yoneta, T., and Mihara, H.: Activation of plasma fibrinolysis after intrarectal administration of high molecular weight urokinase and its derivative. Acta Haematol., 70, 289-295 (1983).
10. Sasaki, K., Moriyama, S., Sumi, H., Toki, N., and Robbins, K. C.: The transport of 125I-labeled human high molecular weight urokinase across the intestinal tract in a dog model with stimulation of synthesis and/or release of plasminogen activators. Blood, 66, 67-75 (1985).
11. Abe, T., Kazama, M., Kinoshita, T., Naito, I., Ogushi, T., Yoshimura, Y., Teruya, J., and Shimizu, N.: Shift of fibrinolysis system at oral administration of urokinase in human sub- jects: double blind cross over study. Blood Vessels, 13, 472-479 (1982). (in Japanese)
12. Sumi, H., Toki, N., Sasaki, K., and Robbins, K. C.: Oral administration of urokinase. Thromb. Res., 20, 711-714 (1980).
13. Sumi, H., Seiki, M., Morimoto, N., Tsushima, H., Maruyama, M., and Mihara, H.: Plasma fibrinolysis after intraduodenal administration of urokinase in rats. Enzyme, 33, 121-127 (1985).
14. Toki, N., Sumi, H., Sasaki, K., Boreisha, I., and Robbins, K. C.: Transport of urokinase across the intestinal tract of normal human subjects with stimulation of synthesis and/or release of urokinase-type proteins. J. Clin. Invest., 75, 1212-1222 (1985).
15. Mihara, H., Sumi, H., Yoneta, T., Mizumoto, H., Ikeda, R., Seiki, M., and Maruyama, M.: A novel fibrinolytic enzyme extracted from the earthworm, Lumbricus rubellus. Jpn. J. Physiol., 41, 461-472 (1991).
16. Sumi, H., Hamada, H., Tsushima, H., Mihara, H., and Muraki, H.: A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese natto: a typical and popular soybean food in the Japanese diet. Experientia, 43, 1110-1111 (1987).
17. Nakajima, N., Taya, N., and Sumi, H.: Potent fibrinolytic enzyme from the lysate of Katsuwonus pelamis digestive tract (Shiokara): purification and characterization. Biosci. Biotech. Biochem., 57, 1604-1605 (1993).
18. Kim, W. K., Choi, K. H., Kim, Y. T., Park, H. H., Choi, J. Y., Lee, Y. S., Oh, H. I., Kwon, I. B., and Lee, S. Y.: Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang. Appl. Environ. Microbiol., 62, 2482-2488 (1996).
19. Astrup, T. and Müllertz, S.: The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys., 40, 346-351 (1952).
20. Bradford, M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976).
21. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
22. Katunuma, N., Umezawa, H., and Holzer, H.: Proteinase inhibitors: medical and biological aspects, p. 165-179. Japan Scientific Societies Press, Tokyo (1983).
23. Nakamura, T., Yamagata, Y., and Ichishima, E.: Nucleotide sequence of the subtilisin NAT gene, aprN, of Bacillus subtilis (Natto). Biosci. Biotech. Biochem., 56, 1869-1871 (1992).
24. Sumi, H., Hamada, H., Nakanishi, K., and Hiratani, H.: Enhancement of the fibrinolytic activity in plasma by oral administration of NK. Acta Haematol., 84, 139-143 (1990).