Cysteine-scanning analysis of helices TM8, TM9a, and TM9b and intervening loops in the YgfO xanthine permease: A carboxyl group is essential at ASP-276

Journal of Biological Chemistry

Volumn 285, Issue 45, 2010, Pages 35011-35020

  Mermelekas, George ^a   Georgopoulou, Ekaterini ^a   Kallis, Alexander ^a   Botou, Maria ^a   Vlantos, Vassilios ^a   Frillingos, Stathis ^a  

^a UNIVERSITY OF IOANNINA   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID RESIDUES; AMINO ACID SEQUENCE; AMPHIPATHIC; CARBOXYL GROUPS; CYS RESIDUES; CYSTEINE-SCANNING; FUNCTIONAL MUTANTS; HIGH ACTIVITY; LOW LEVEL; METHYLXANTHINE; NUCLEOBASES; STEADY STATE; TRANSPORT MECHANISM; WILD TYPES;

BINDING ENERGY; ESCHERICHIA COLI;

AMINO ACIDS;

2 THIOXANTHINE; 3 METHYLXANTHINE; 6 THIOXANTHINE; 8 METHYLXANTHINE; ALANINE; ASPARTIC ACID; CARBOXYL GROUP; CYSTEINE; GLUTAMIC ACID; GLYCINE; N ETHYLMALEIMIDE; NUCLEOBASE ASCORBATE TRANSPORTER; NUCLEOBASE TRANSPORTER; PERMEASE; PROLINE; PURINE; THREONINE; UNCLASSIFIED DRUG; XANTHINE; XANTHINE DERIVATIVE; XANTHINE PERMEASE;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CELL MEMBRANE; CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE MUTATION; GENETIC CONSERVATION; MOLECULAR RECOGNITION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN MOTIF; SEQUENCE HOMOLOGY; STEADY STATE; TRANSPORT KINETICS; WILD TYPE;

AMINO ACID MOTIFS; AMINO ACID SUBSTITUTION; CELL MEMBRANE; CYSTEINE; ESCHERICHIA COLI K12; ESCHERICHIA COLI PROTEINS; MUTATION, MISSENSE; NUCLEOBASE TRANSPORT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 78049395093     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.170415     Document Type: Article

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