Role of intramembrane polar residues in the YgfO xanthine permease. HIS-31 and ASN-93 are crucial for affinity and specificity, and ASP-304 and GLU-272 are irreplaceable

Journal of Biological Chemistry

Volumn 284, Issue 36, 2009, Pages 24257-24268

  Karena, Ekaterini ^a   Frillingos, Stathis ^a  

^a UNIVERSITY OF IOANNINA   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

CYS RESIDUES; HIGH ACTIVITY; IMIDAZOLE MOIETY; NUCLEOBASES; POLAR RESIDUES; PURINE BASIS; SITE DIRECTED MUTAGENESIS; TRANSMEMBRANE HELICES; WILD TYPES;

ESCHERICHIA COLI;

MUTAGENESIS;

ARGININE; ASPARAGINE; ASPARTIC ACID; CYSTEINE; GLUTAMIC ACID; GLUTAMINE; HISTIDINE; LYSINE; N ETHYLMALEIMIDE; PERMEASE; PROTEIN YGFO; SERINE; THREONINE; UNCLASSIFIED DRUG; XANTHINE; XANTHINE DERIVATIVE;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL MEMBRANE; BACTERIUM MUTANT; BINDING AFFINITY; CONTROLLED STUDY; ENZYME SPECIFICITY; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; PREDICTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; SITE DIRECTED MUTAGENESIS; WILD TYPE;

AMINO ACID SUBSTITUTION; ASPARAGINE; ASPARTIC ACID; ESCHERICHIA COLI K12; ESCHERICHIA COLI PROTEINS; EVOLUTION, MOLECULAR; GLUTAMIC ACID; HISTIDINE; MEMBRANE TRANSPORT PROTEINS; MODELS, BIOLOGICAL; MUTATION, MISSENSE; NUCLEOBASE TRANSPORT PROTEINS; PROTEIN STRUCTURE, SECONDARY; SUBSTRATE SPECIFICITY; XANTHINE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 69949131591     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.030734     Document Type: Article

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