Structure of a eukaryotic CLC transporter defines an intermediate state in the transport cycle

Science

Volumn 330, Issue 6004, 2010, Pages 635-641

  Feng, Liang ^a   Campbell, Ernest B ^a   Hsiung, Yichun ^a   MacKinnon, Roderick ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORIDE CHANNEL; CYSTATHIONINE BETA SYNTHASE; ELECTROLYTE; GLUTAMIC ACID; MEMBRANE PROTEIN; PROTEIN CLC; UNCLASSIFIED DRUG;

CHLORIDE; CYTOLOGY; ELECTROLYTE; EUKARYOTE; MUSCLE; PH; PROKARYOTE; PROTEIN; STOICHIOMETRY;

ARTICLE; CELL MEMBRANE TRANSPORT; CELL ORGANELLE; CHLORIDE TRANSPORT; CONFORMATIONAL TRANSITION; CYTOPLASM; ELECTRIC POTENTIAL; ELECTROLYTE TRANSPORT; EUKARYOTE; ION TRANSPORT; MUSCLE CELL; PH; PRIORITY JOURNAL; PROKARYOTE; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN STRUCTURE; PROTEIN TRANSPORT; PROTON TRANSPORT; STOICHIOMETRY;

ALGAE, RED; ALGAL PROTEINS; ANIMALS; ANTIPORTERS; BINDING SITES; CELL LINE; CELL MEMBRANE; CHLORIDE CHANNELS; CHLORIDES; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; CYSTATHIONINE BETA-SYNTHASE; CYTOPLASM; GLUTAMIC ACID; ION CHANNEL GATING; ION TRANSPORT; MODELS, BIOLOGICAL; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; PROTONS;

EUKARYOTA; PROKARYOTA;

EID: 78049362741     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1195230     Document Type: Article

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59. We thank the staff at beamline X29 (National Synchrotron Light Source, Brookhaven National Laboratory) and K. R. Rajashankar and K. Perry at beamline 24ID-C (Advanced Photon Source, Argonne National Laboratory) for assistance at the synchrotron, members of the MacKinnon laboratory for helpful dicussions, and P. Yuan for comments on the manuscript. R.M. is an Investigator in the Howard Hughes Medical Institute. The x-ray crystallographic coordinates and structure factors have been deposited in the Protein Data Bank with accession identification number 3ORG.