X-ray structure of a CIC chloride channel at 3.0 Å reveals the molecular basis of anion selectivity

Nature

Volumn 415, Issue 6869, 2002, Pages 287-294

  Dutzler, Raimund ^a   Campbell, Ernest B ^a   Cadene, Martine ^a   Chait, Brian T ^a   MacKinnon, Roderick ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL MEMBRANES; CHLORINE; ESCHERICHIA COLI; MUSCLE; NEGATIVE IONS; STRUCTURE (COMPOSITION); X RAYS;

ANTIPARRELLEL ARCHITECTURE;

CHANNEL FLOW;

CHLORIDE CHANNEL; CHLORIDE ION; MEMBRANE PROTEIN; POTASSIUM ION; SODIUM ION;

ANION; BIOCHEMISTRY; CHLORIDE;

ARTICLE; CATALYSIS; CELL MEMBRANE TRANSPORT; ESCHERICHIA COLI; GENETIC DISORDER; ION TRANSPORT; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN STRUCTURE; SALMONELLA ENTERICA; SEQUENCE ANALYSIS; SEROTYPE;

AMINO ACID SEQUENCE; ANIMALS; ANIONS; BACTERIAL PROTEINS; CHLORIDE CHANNELS; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; ION TRANSPORT; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SALMONELLA TYPHIMURIUM; SEQUENCE HOMOLOGY, AMINO ACID;

ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA; SALMONELLA; SALMONELLA ENTERICA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM;

EID: 0037122805     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/415287a     Document Type: Article

References (50)

1. + conduction and selectivity
2. + channel-Fab complex at 2.0 Å resolution
3. The CLC chloride channel family
4. A decade of CLC chloride channels: Structure, mechanism, and many unsettled questions
5. Ion permeation and selectivity in CIC-type chloride channels
6. Multimeric structure of CIC-1 chloride channel revealed by mutations in dominant myotonia congenita (Thomsen)
7. A voltage-gated anion channel from the electric organ of Torpedo californica
8. Open-state substructure of single chloride channels from Torpedo electroplax
9. Dimeric structure of single chloride channels from Torpedo electroplax
10. Primary structure of Torpedo marmorata chloride channel isolated by expression cloning in Xenopus oocytes
11. Two physically distinct pores in the dimeric CIC- 0 chloride channel
12. Homodimeric architecture of a CIC-type chloride ion channel
13. Pores formed by single subunits in mixed dimers of different CLC chloride channels
14. High-level expression, functional reconstitution, and quaternary structure of a prokaryotic CIC-type chloride channel
15. Projection structure of a CIC-type chloride channel at 6.5 Å resolution
16. - channel from Torpedo electroplax
17. Permeation and block of the skeletal muscle chloride channel, CIC- 1, by foreign anions
18. Interaction of hydrophobic anions with the rat skeletal muscle chloride channel CIC-1: Effects on permeation and gating
19. Steady-state coupling of ion-channel conformations to a transmembrane ion gradient
20. Gating of the voltage-dependent chloride channel CIC-0 by the permeant anion
21. - channel
22. Inward rectification in CIC-0 chloride channels caused by mutations in several protein regions
23. Analysis of a protein region involved in permeation and gating of the voltage-gated Torpedo chloride channel CIC-0
24. Formation of CLC-0 chloride channels from separated transmembrane and cytoplasmic domains
25. High-level expression, functional reconstitution, and quaternary structure of a prokaryotic CIC-type chloride channel
26. Not your average density
27. Crystal structures explain functional properties of two E. coli porins
28. Structural determinants of water permeation through aquaporin- 1
29. Structure of a glycerol-conducting channel and the basis for its selectivity
30. Electron-crystallographic refinement of the structure of bacteriorhodopsin
31. Pore-forming segments in voltage-gated chloride channels
32. A mutation in autosomal dominant myotonia congenita affects pore properties of the muscle chloride channel
33. + channel: Electrostatic stabilization of monovalent cations
34. Potential energy barriers to ion transport within lipid bilayers
35. Electrostatic potentials at membrane-solution interfaces
36. Electrostatic modeling of ion pores. II. Effects attributable to the membrane dipole potential
37. Processing of X-ray diffraction data collected in oscillation mode
38. The CCP4 Suite: Programs for X-ray crystallography
39. Methods used in the structure determination of bovine mitochondrial F 1 ATPase
40. Locating proper non-crystallographic symmetry in low-resolution electron-density maps with the program GETAX
41. AMoRe: An automated package for molecular replacement
42. Improved methods for building protein models in electron density maps and the location of errors in these models
43. Crystallography & NMR system: New software suite for macromolecular structure determination
44. DM: An automated procedure for phase improvement by density modification
45. Rapid grid-based construction of the molecular surface and the use of induced surface charge to calculate reaction field energies: Application to the molecular systems and geometric objects
46. All-atom empirical potential for molecular modeling and dynamics studies of proteins
47. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice
48. Reduced surface: An efficient way to compute molecular surfaces