Isolation of a human anti-epidermal growth factor receptor Fab antibody, EG-19-11, with subnanomolar affinity from naïve immunoglobulin repertoires using a hierarchical antibody library system

Immunology Letters

Volumn 134, Issue 1, 2010, Pages 55-61

  Hur, Byung Ung ^a   Yoon, Jae Bong ^a   Liu, Li Kun ^a   Cha, Sang Hoon ^a  

^a Kangwon National University   (South Korea)

Author keywords

Antibody library; Dual vector system; EGFR; Ex12 helper phage; Phage display technology

Indexed keywords

BOVINE SERUM ALBUMIN; CETUXIMAB; EG 19 11; EPIDERMAL GROWTH FACTOR; EPIDERMAL GROWTH FACTOR RECEPTOR; EPIDERMAL GROWTH FACTOR RECEPTOR ANTIBODY; EPIDERMAL GROWTH FACTOR RECEPTOR ANTIBODY IMMUNOGLOBULIN F(AB) FRAGMENT FUSION PROTEIN; EPITOPE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN KAPPA CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MALTOSE BINDING PROTEIN; RECOMBINANT INTERLEUKIN 15; UNCLASSIFIED DRUG;

ANTIBODY AFFINITY; ANTIBODY ISOLATION; ANTIBODY LIBRARY; ARTICLE; BINDING KINETICS; BIOPANNING; CELL LINE; CELL STRAIN A 431; CONTROLLED STUDY; ENDOCYTOSIS; INTERNALIZATION; MOLECULAR CLONING; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; BIOTINYLATION; BLOTTING, WESTERN; CELL LINE, TUMOR; ESCHERICHIA COLI; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; JURKAT CELLS; MICE; NIH 3T3 CELLS; PEPTIDE LIBRARY; PHOSPHORYLATION; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECOMBINANT PROTEINS; TYROSINE;

EID: 78049304341     PISSN: 01652478     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.imlet.2010.08.009     Document Type: Article

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