Mitochondria-specific transgenic overexpression of connexin-43 simulates preconditioning-induced cytoprotection of stem cells

Cardiovascular Research

Volumn 88, Issue 2, 2010, Pages 277-286

  Lu, Gang ^a   Haider, Husnain Kh ^a   Porollo, Aleksey ^a   Ashraf, Muhammad ^a  

^a UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

Apoptosis; Connexin 43; Heart; IGF 1; Mitochondria; Sca 1+

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; BH3 PROTEIN; CASPASE 3; CONNEXIN 43; CYTOCHROME C; GLUCOSE; LACTATE DEHYDROGENASE; OXYGEN; SOMATOMEDIN C; STEM CELL ANTIGEN 1;

ALPHA HELIX; ANIMAL EXPERIMENT; ARTICLE; BONE MARROW CELL; CELL PROTECTION; CELL SURVIVAL; CLONE; CONTROLLED STUDY; CYTOSOL; GENE OVEREXPRESSION; GENE TRANSLOCATION; MALE; MITOCHONDRIAL MEMBRANE; MOUSE; NICK END LABELING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; STEM CELL;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, LY; APOPTOSIS; CELLS, CULTURED; CONNEXIN 43; CONSERVED SEQUENCE; CYTOCHROMES C; CYTOPROTECTION; INSULIN-LIKE GROWTH FACTOR I; MALE; MEMBRANE PROTEINS; MICE; MICE, INBRED C57BL; MITOCHONDRIA; MITOCHONDRIAL MEMBRANES; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN KINASE INHIBITORS; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; PROTO-ONCOGENE PROTEINS C-BCL-2; RECOMBINANT FUSION PROTEINS; STEM CELLS; STRUCTURE-ACTIVITY RELATIONSHIP; TIME FACTORS; TRANSFECTION; UP-REGULATION;

EID: 77958097322     PISSN: 00086363     EISSN: 17553245     Source Type: Journal    
DOI: 10.1093/cvr/cvq293     Document Type: Article

References (31)

1. Duffy HS, Fort AG, Spray DC Cardiac connexins: genes to nexus. Adv Cardiol 2006; 42:1-17.
2. Rodriguez-Sinovas A, Cabestrero A, Lopez D, Torre I, Morente M, Abellan A et al. The modulatory effects of connexin-43 on cell death/survival beyond cell coupling. Prog Biophys Mol Biol 2007;94:219-232.
3. Mesnil M, Krutovskikh V, Piccoli C, Elfgang C, Traub O, Willecke K et al. Negative growth control of HeLa cells by connexin genes: connexin species specificity. Cancer Res 1995;55:629-639.
4. Doble BW, Dang X, Ping P, Fandrich RR, Nickel BE,Jin Y et al. Phosphorylation of serine 262 in the gap junction protein connexin-43 regulates DNA synthesis in cell-cell contact forming cardiomyocytes. J Cell Sci 2004;117:507-514.
5. Boengler K, Dodoni G, Rodriguez-Sinovas A, Cabestrero A, Ruiz-Meana M, Gres P et al. Connexin-43 in cardiomyocyte mitochondria and its increase by ischemic pre-conditioning. Cardiovasc Res 2005;67:234-244.
6. Heinzel FR, Luo Y, Li X, Boengler K, Buechert A, Garcia-Dorado D et al. Impairment of diazoxide-induced formation of reactive oxygen species and loss of Cardioprotection in connexin-43 deficient mice. Circ Res 2005;97:583-586.
7. Rodriguez-Sinovas A, Boengler K, Cabestrero A, Gres P, Morente M, Ruiz-Meana M et al. Translocation of connexin-43 to the inner mitochondrial membrane of cardio-myocytes through the heat shock protein 90-dependentTOM pathway and its impor-tance for cardioprotection. Circ Res 2006;99:93-101.
8. + stem cell survival and engraftment in the infarcted heart: dual role for preconditioning-induced connexin-43. Circulation 2009; 119:2587-2596.
9. Won Kim H, Haider HK, Jiang S, Ashraf M. Ischemic preconditioning augments survi-val of stem cells via miR-210 expression by targeting caspase-8-associated protein-2. J Biol Chem 2009;284:33161-33168.
10. Finn RD, Tate J, Mistry J, Coggill PC, Sammut SJ, Hotz HR et al. The Pfam protein families database. Nucleic Acids Res 2008;36:D281-D288.
11. Blaineau SV, Aouacheria A. BCL2DB: moving 'helix-bundled' BCL-2 family members to their database. Apoptosis 2009;14:923-925.
12. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990;215:403-410.
13. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007;23:2947-2948.
14. Adamczak R, Porollo A, Meller J. Accurate prediction of solvent accessibility using neural networks-based regression. Proteins 2004;56:753-767.
15. Cao B, Porollo A, Adamczak R, Jarrell M, Meller J. Enhanced recognition of protein transmembrane domains with prediction-based structural profiles. Bioinformatics 2006;22:303-309.
16. WebsterJ, PragerD, Melmed S. Insulin-likegrowthfactor-1 activation ofextracellular signal-related kinase-1 and-2 in growth hormone-secreting cells. Mol Endocrinol 1994; 8:539-544.
17. Petros AM, Olejniczak ET, FesikSW. Structural biology of the Bcl-2 family of proteins. Biochim Biophys Acta 2004;1644:83-94.
18. Fleischer A, Ayllon V, Dumoutier L, Renauld JC, Rebollo A. Proapoptotic activity of ITM2B(s), a BH3-only protein induced upon IL-2-deprivation which interacts with Bcl-2. Oncogene 2002;21:3181-3189.
19. TUPR. The anti-apoptotic molecules Bcl-xL and Bcl-w target protein phos-phatase 1alpha to Bad. Nucleic Acids Res 2009;37:D169-174.
20. Schwanke U, Konietzka I, Duschin A, Li X, Schulz R, Heusch G. No ischemic precon-ditioning in heterozygous connexin-43-deficient mice. Am J Physiol Heart Circ Physiol 2002;283:H1740-H1742.
21. Padilla F, Garcia-Dorado D, Rodriguez-Sinovas A, Ruiz-Meana M, Inserte J, Soler-Soler J. Protection afforded by ischemic preconditioning is not mediated by effects on cell-to-cell electrical coupling during myocardial ischemia-reperfusion. Am J Physiol Heart Circ Physiol 2003;285:H1909-H1916.
22. Li X, Heinzel FR, Boengler K, Schulz R, Heusch G. Role of connexin-43 in ischemic preconditioning does not involve intercellular communication through gap junctions. J Mol Cell Cardiol 2004;36:161-163.
23. Rodriguez-Sinovas A, Sanchez JA, Gonzalez-Loyola A, Barba I, Morente M, Aguilar R et al. Effects of substitution of Cx-43 by Cx-32 on myocardial energy metabolism, tol-erance to ischaemia and preconditioning protection. J Physiol 2010;588:1139-1151.
24. Ljubkovic M, Marinovic J, FuchsA, BosnjakZJ, Bienengraeber M. Targeted expression of Kir6.2 in mitochondria confers protection against hypoxic stress. J Physiol 2006;577: 17-29.
25. Hausenloy DJ, Tsang A, Mocanu MM, Yellon DM. Ischemic preconditioning protects by activating prosurvival kinases at reperfusion. Am J Physiol Heart Circ Physiol 2005; 288:H971-H976.
26. Naitoh K, Ichikawa Y, Miura T, Nakamura Y, Miki T, Ikeda Y et al. MitoKATP channel activation suppresses gap junction permeability in the ischemic myocardium by an ERK-dependent mechanism. Cardiovasc Res 2006;70:374-383.
27. Miro-Casas E, Ruiz-Meana M, Agullo E, Stahlhofen S, Rodriguez-Sinovas A, Cabestrero A et al. Connexin-43 in cardiomyocyte mitochondria contributes to mito-chondrial potassium uptake. Cardiovasc Res 2009;83:747-756.
28. Kluck RM, Bossy-Wetzel E, Green DR, Newmeyer DD. The release ofcytochrome-c from mitochondria: a primary site for Bcl-2 regulation of apoptosis. Science 1997;275: 1132-1136.
29. Yang J, Liu X, Bhalla K, Kim CN, Ibrado AM, Cai J et al. Prevention of apoptosis by Bcl-2: release of cytochrome-c from mitochondria blocked. Science 1997;275: 1129-1132.
30. Chittenden T, Flemington C, Houghton AB, Ebb RG, Gallo GJ, Elangovan B et al. A conserved domain in Bak, distinct from BH1 and BH2, mediates cell death and protein bindingfunctions. Embo J 1995;14:5589-5596.
31. Kelekar A, Thompson CB. Bcl-2-family proteins: the role of the BH3 domain in apop-tosis. Trends Cell Biol 1998;8:324-330.