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Volumn 162, Issue 7, 2010, Pages 2098-2110

Expression, one-step purification, and immobilization of HaloTag TM fusion proteins on chloroalkane-functionalized magnetic beads

(5) Motejadded, Hassan a Kranz, Bertolt b Berensmeier, Sonja c Franzreb, Matthias c Altenbuchner, Josef a

a UNIVERSITY OF STUTTGART (Germany)

b UNIVERSITY OF HOHENHEIM (Germany)

c KARLSRUHE INSTITUTE OF TECHNOLOGY (Germany)

Author keywords

Dehalogenase; Enzyme immobilization; Expression; Fusion protein; HaloTag ; Magnetic bead

Indexed keywords

BIOSYNTHESIS; GENES; LIGANDS; MAGNETISM; PURIFICATION;

COVALENT COUPLINGS; DEHALOGENASE; DEHALOGENASE GENES; EXPRESSION; FUSION PROTEINS; MAGNETIC BEADS; MICROMETER RANGES; ONE-STEP PURIFICATION;

ENZYME IMMOBILIZATION;

ALKANE DERIVATIVE; HYBRID PROTEIN; TRIACYLGLYCEROL LIPASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; GENE FUSION; GENETIC LINKAGE; MAGNET; NONHUMAN; PROTEIN EXPRESSION; PROTEIN IMMOBILIZATION; PROTEIN PURIFICATION; SYNTHESIS;

EID: 77958085608 PISSN: 02732289 EISSN: None Source Type: Journal
DOI: 10.1007/s12010-010-8985-1 Document Type: Article

Times cited : (22)

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