One-step purification, covalent immobilization, and additional stabilization of poly-his-tagged proteins using novel heterofunctional chelate-epoxy supports

Biotechnology and Bioengineering

Volumn 76, Issue 3, 2001, Pages 269-276

  Mateo, Cesar ^a   Fernández Lorente, Gloria ^a   Cortés, Estrella ^b   Garcia, José L ^b   Fernández Lafuente, Roberto ^a,b   Guisan, José M ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

^b CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

Author keywords

Epoxy supports; Glutaryl acylase; Immobilized metal chelate affinity chromatography; Multipoint covalent attachment; Selective immobilization of enzymes

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; PH EFFECTS; PROTEINS; PURIFICATION;

CHELATE EPOXY SUPPORTS (CES);

BIOTECHNOLOGY;

AMIDASE; CHELATE; EPOXIDE; POLYHISTIDINE; PROTEIN;

ADSORPTION; AFFINITY CHROMATOGRAPHY; ARTICLE; CHELATION; COVALENT BOND; PH; PROTEIN IMMOBILIZATION; PROTEIN PURIFICATION; PROTEIN STABILITY;

EID: 0035148782     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10019     Document Type: Article

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