메뉴 건너뛰기




Volumn 17, Issue 8, 2010, Pages 1048-1052

Determination of malic acid using a malate dehydrogenase reactor after purification and immobilization in non-denaturing conditions and staining with ponceau S

Author keywords

Enzyme reactor; Immobilization; Malate dehydrogenase; Malic acid; Ponceau S; Two dimensional electrophoresis

Indexed keywords

AZO COMPOUND; IMMOBILIZED ENZYME; MALATE DEHYDROGENASE; MALIC ACID; MALIC ACID DERIVATIVE; PONCEAU S;

EID: 77958026615     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/092986610791498867     Document Type: Article
Times cited : (14)

References (14)
  • 1
    • 33847677249 scopus 로고    scopus 로고
    • Hydrolytic activity of lipase on anion-exchange solid phase column after separation and electrotransfer by nondenaturing electrophoresis
    • Shimazaki, Y. Hydrolytic activity of lipase on anion-exchange solid phase column after separation and electrotransfer by nondenaturing electrophoresis. J. Biochem. Biophys.Methods, 2007, 70, 487-491.
    • (2007) J. Biochem. Biophys.Methods , vol.70 , pp. 487-491
    • Shimazaki, Y.1
  • 2
    • 70350123701 scopus 로고    scopus 로고
    • Production of enzyme reactors after separation by non-denaturing two-dimensional electrophoresis and immobilization on membrane
    • Shimazaki, Y.; Kuroda, T. Production of enzyme reactors after separation by non-denaturing two-dimensional electrophoresis and immobilization on membrane. Biotechnol. Lett., 2009, 31, 1545-1549.
    • (2009) Biotechnol. Lett. , vol.31 , pp. 1545-1549
    • Shimazaki, Y.1    Kuroda, T.2
  • 3
    • 43649108590 scopus 로고    scopus 로고
    • Simultaneous immobilization of dehydrogenases on polyvinylidene difluoride resin after separation by non-denaturing two-dimensional electrophoresis
    • Shimazaki, Y.; Kadota, M. Simultaneous immobilization of dehydrogenases on polyvinylidene difluoride resin after separation by non-denaturing two-dimensional electrophoresis. Anal. Chim. Acta., 2008, 618, 116-119.
    • (2008) Anal. Chim. Acta. , vol.618 , pp. 116-119
    • Shimazaki, Y.1    Kadota, M.2
  • 4
    • 35649024331 scopus 로고    scopus 로고
    • Analysis of lipid hydrolytic activity by esterase on blotting membrane followed by separation using non-denaturing two-dimensional gel electrophoresis
    • Shimazaki, Y.; Miyamoto, M. Analysis of lipid hydrolytic activity by esterase on blotting membrane followed by separation using non-denaturing two-dimensional gel electrophoresis. Biotechnol. Bioeng., 2007, 98, 732-736.
    • (2007) Biotechnol. Bioeng. , vol.98 , pp. 732-736
    • Shimazaki, Y.1    Miyamoto, M.2
  • 5
    • 0022451826 scopus 로고
    • Reversible staining and peptide mapping of proteins transferred to nitrocellulose after separation by sodiumdodecylsulfate polyacrylamide gel electrophoresis
    • Salinovich, O.; Montelaro, R.C. Reversible staining and peptide mapping of proteins transferred to nitrocellulose after separation by sodiumdodecylsulfate polyacrylamide gel electrophoresis. Anal. Biochem., 1986, 156, 341-347.
    • (1986) Anal. Biochem. , vol.156 , pp. 341-347
    • Salinovich, O.1    Montelaro, R.C.2
  • 7
    • 0142151382 scopus 로고    scopus 로고
    • Analysis of the activity and identification of enzymes after separation of cytosol proteins in mouse liver by microscale nondenaturing twodimensional electrophoresis
    • Shimazaki, Y.; Sugawara, Y.; Ohtsuka, Y.; Manabe, T. Analysis of the activity and identification of enzymes after separation of cytosol proteins in mouse liver by microscale nondenaturing twodimensional electrophoresis. Proteomics, 2003, 3, 2002-2007.
    • (2003) Proteomics , vol.3 , pp. 2002-2007
    • Shimazaki, Y.1    Sugawara, Y.2    Ohtsuka, Y.3    Manabe, T.4
  • 9
    • 0016711037 scopus 로고
    • High resolution two-dimensional electrophoresis of proteins
    • O'Farrell, P.H. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem., 1975, 250, 4007-4021.
    • (1975) J. Biol. Chem. , vol.250 , pp. 4007-4021
    • O'Farrell, P.H.1
  • 10
    • 0023430927 scopus 로고
    • Internal amino acid sequence analysis of proteins separated by one-or twodimensional gel electrophoresis after in situ protease digestion on nitrocellulose
    • Aebersold, R.; Leavitt, J.; Saavedra, R; Hood, L.; Kent, S. Internal amino acid sequence analysis of proteins separated by one-or twodimensional gel electrophoresis after in situ protease digestion on nitrocellulose. Proc. Natl. Acad, Sci. USA, 1987, 84, 6970-6974.
    • (1987) Proc. Natl. Acad, Sci. USA , vol.84 , pp. 6970-6974
    • Aebersold, R.1    Leavitt, J.2    Saavedra, R.3    Hood, L.4    Kent, S.5
  • 11
    • 0034083942 scopus 로고    scopus 로고
    • Direct blue 71 staining of proteins bound to blotting membranes
    • Hong, H.Y.; Yoo, G.S.; Choi, J.K. Direct blue 71 staining of proteins bound to blotting membranes. Electrophoresis, 2000, 21, 841-845.
    • (2000) Electrophoresis , vol.21 , pp. 841-845
    • Hong, H.Y.1    Yoo, G.S.2    Choi, J.K.3
  • 12
    • 0021902861 scopus 로고
    • Properties and function of malate enzyme from dicentrarchus labrax L. Liver
    • Iniesta, M.G.; Cano, M.J.; Garrido-Pertierra, A. Properties and function of malate enzyme from dicentrarchus labrax L. liver. Comp. Biochem. Physiol., 1985, 80B, 35-39.
    • (1985) Comp. Biochem. Physiol. , vol.80 B , pp. 35-39
    • Iniesta, M.G.1    Cano, M.J.2    Garrido-Pertierra, A.3
  • 13
    • 0020583886 scopus 로고
    • Polyol-pathway enzymes of human brain. Partial purification and properties of sorbitol dehydrogenase
    • O'Brien, M.M.; Schofield, P.J.; Edwards, M.R. Polyol-pathway enzymes of human brain. Partial purification and properties of sorbitol dehydrogenase. Biochem. J., 1983, 211, 81-90.
    • (1983) Biochem. J. , vol.211 , pp. 81-90
    • O'Brien, M.M.1    Schofield, P.J.2    Edwards, M.R.3
  • 14
    • 0033555285 scopus 로고    scopus 로고
    • Structural basis of substrate specifitciy in malate deyhydrogenase s: Crystal structure of a ternary complex of porcine cytoplasmic malate dehydrogenase, mm-ketomalonateand tetrahydroNAD
    • Chapman, A.D.M.; Cortes, A.; Dafforn, T.R.; Clarke, A.R.; Brady, R.L. Structural basis of substrate specifitciy in malate deyhydrogenase s: Crystal structure of a ternary complex of porcine cytoplasmic malate dehydrogenase, mm-ketomalonateand tetrahydroNAD. J. Mol. Biol., 1999, 285, 703-712.
    • (1999) J. Mol. Biol. , vol.285 , pp. 703-712
    • Chapman, A.D.M.1    Cortes, A.2    Dafforn, T.R.3    Clarke, A.R.4    Brady, R.L.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.