Hydrolytic activity of lipase on anion-exchange solid phase column after separation and electrotransfer by non-denaturing electrophoresis

Journal of Biochemical and Biophysical Methods

Volumn 70, Issue 3, 2007, Pages 487-491

  Shimazaki, Youji ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Non denaturing 2 DE; Phosphatidylcholine; Phospholipase C alpha

Indexed keywords

1 NAPHTHYL ACETATE; PHOSPHOLIPASE C; PHOSPHOLIPASE C ALPHA; RESIN; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANIMAL TISSUE; ANION EXCHANGE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ISOLATION; HYDROLYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RETINA; STAINING; SWINE; TWO DIMENSIONAL GEL ELECTROPHORESIS;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HYDROLYSIS; ION EXCHANGE RESINS; PEPTIDE FRAGMENTS; PHOSPHATIDYLCHOLINES; PHOSPHOLIPASE C; RETINA; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SWINE; TANDEM MASS SPECTROMETRY;

SUS;

EID: 33847677249     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbbm.2006.11.010     Document Type: Article

References (14)

1. Shimazaki Y., Sugawara Y., Ohtsuka Y., and Manabe T. Analysis of the activity and identification of enzymes after separation of cytosol proteins in mouse liver by microscale nondenaturing two-dimensional electrophoresis. Proteomics 3 (2003) 2002-2007
2. Shimazaki Y., Hiraka Y., Uesugi M., and Manabe T. Simultaneous analysis of esterase and transferase in cytosol proteins from bovine retina by using microscale non-denaturing two-dimensional electrophoresis. Biochim Biophys Acta 1696 (2004) 51-57
3. Shimazaki Y., and Manabe T. Detection of activity and mass spectrometric identification of mouse liver carboxylesterase and aldehyde dehydrogenase separated by non-denaturing two-dimensional electrophoresis after extraction with detergents. Biochim Biophys Acta 1749 (2005) 95-101
4. Manabe T., Takahashi Y., and Okuyama T. An electroblotting apparatus for multiple replica technique and identification of human serum proteins on micro two-dimensional gels. Anal Biochem 143 (1984) 39-45
5. Shimazaki Y., and Manabe T. Application of two-dimensional electrophoresis without denaturing agents to comparative study of vertebrate plasma proteins. Jpn J Electrophor 42 (1998) 13-18
6. Tompkins T.A., and Moscarello M.A. A 57-kDa phosphatidylinositol-specific phospholipase C from bovine brain. J Biol Chem 266 (1991) 4228-4236
7. Kuramitsu S., and Hamaguchi K. Protein constants. In: Yamakawa T., et al. (Ed). Seikagaku data book I (1979), Tokyo kagaku Doujin, Tokyo 92-135
8. Fenn J.B., Mann M., Meng C.K., Wong S.F., and Whitehouse C.M. Electrospray ionization for mass spectrometry of large biomolecules. Science 246 (1989) 64-71
9. Henzel W.J., Billeci T.M., Stults J.T., Wong S.C., Grimeley C., and Watanabe C. Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence database. Proc Natl Acad Sci 90 (1993) 5011-5015
10. Corthals G.L., Gygi S.P., Aebersold R., and Patterson S.D. Identification of proteins by mass spectrometry. In: Rabilloud T. (Ed). Proteome research: two-dimensional gel electrophoresis and identification methods (2000), Springer Verlag, Berlin Heidelberg 197-231
11. Johnston K.J., and Ashford A.E. A simultaneous-coupling azo dye method for the quantitative assay of esterase using α-naphthyl acetate as substrate. Histochem J 12 (1980) 221-234
12. Taylor G.D., Fee J.A., Silbert D.F., and Hofmann S.L. PI-specific phospholipase C "α" from sheep seminal vesicles is a proteolytic fragment of PI-PLCδ. Biochem Biophys Res Commun 188 (1992) 1176-1183
13. Zschörnig O., Pietsch M., Süβ R., Schiller J., and Gütschow M. Cholesterol esterase action on human high density lipoproteins and inhibition studies: detection by MALDI-TOF MS. J Lipid Res 46 (2005) 803-811
14. Shimazaki Y., Kishi H., Mori M., Yasuda C., and Manabe T. Analysis of hydrolytic activity of phospholipase Cα from porcine retina on retinyl ester and phosphatidilcholine using non-denaturing two-dimensional electrophoresis and mass spectrometry. J Chromatogr B 843 (2006) 42-46