Production of enzyme reactors after separation by non-denaturing two-dimensional electrophoresis and immobilization on membrane

Biotechnology Letters

Volumn 31, Issue 10, 2009, Pages 1545-1549

  Shimazaki, Youji ^a   Kuroda, Takahiro ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Carboxylesterase; Malate dehydrogenase; MALDI TOF MS; Phosphatidylcholine; Reverse staining

Indexed keywords

CARBOXYLESTERASE; MALATE DEHYDROGENASE; MALDI-TOF MS; PHOSPHATIDYLCHOLINE; REVERSE STAINING;

DESORPTION; ELECTROPHORESIS; ENZYME IMMOBILIZATION; MASS SPECTROMETERS; MASS SPECTROMETRY; MEMBRANES; SALTS; SEPARATION; TWO DIMENSIONAL; ZINC;

ENZYMES;

CARBOXYLESTERASE; IMMOBILIZED ENZYME; MALATE DEHYDROGENASE;

ANIMAL; ARTICLE; BIOREACTOR; ENZYMOLOGY; ISOLATION AND PURIFICATION; LIVER; MEMBRANE; METABOLISM; MOUSE;

ANIMALS; BIOREACTORS; CARBOXYLESTERASE; ENZYMES, IMMOBILIZED; LIVER; MALATE DEHYDROGENASE; MEMBRANES; MICE;

EID: 70350123701     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0041-2     Document Type: Article

References (9)

1. Corthals GL, Gygi SP, Aebersold R, Patterson SD (2000) Identification of proteins by mass spectrometry. In: Rabilloud T (ed) Proteome research. Springer-Verlag, Berlin, pp 197-231.
2. Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM (1989) Electrospray ionization for mass spectrometry of large biomolecules. Science 246:64-71.
3. Hardy E, Castellanos-Serra LR (2004) "Reverse-staining" of biomolecules in electrophoresis gels: analytical and micropreparative applications. Anal Biochem 328:1-13.
4. Johnston KJ, Ashford AE (1980) A simultaneous-coupling azo dye method for the quantitative assay of esterase using α-naphthyl acetate as substrate. Histochem J 12:221-234.
5. Salinovich O, Montelaro RC (1986) Reversible staining and peptide mapping of proteins transferred to nitrocellulose after separation by sodium dodecylsulfate polyacrylamide gel electrophoresis. Anal Biochem 156:341-347.
6. Shimazaki Y (2007) Hydrolytic activity of lipase on anion-exchange solid phase column after separation and electrotransfer by non-dedenaturing electrophoresis. J Biochem Biophys Methods 70:487-491.
7. Shimazaki Y, Kadota M (2008) Simultaneous immobilization of dehydrogenases on polyvinylidene difluoride resin after separation by non-denaturing two-dimensional electrophoresis. Anal Chim Acta 618:116-119.
8. Shimazaki Y, Sugawara Y, Ohtsuka Y, Manabe T (2003) Analysis of the activity and identification of enzymes after separation of cytosol proteins in mouse liver by microscale nondenaturing two-dimensional electrophoresis. Proteomics 3:2002-2007.
9. Zschörnig O, Pietsch M, Süß R, Schiller J, Gütschow M (2005) Cholesterol esterase action on human high density lipoproteins and inhibition studies: detection by MALDI-TOF MS. J Lipid Res 46:803-811.