Simultaneous immobilization of dehydrogenases on polyvinylidene difluoride resin after separation by non-denaturing two-dimensional electrophoresis

Analytica Chimica Acta

Volumn 618, Issue 1, 2008, Pages 116-119

  Shimazaki, Youji ^a   Kadota, Mariko ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Aldehyde dehydrogenase; De novo sequencing; Malate dehydrogenase; Negative stain; Sorbitol dehydrogenase

Indexed keywords

DENATURATION; ELECTROPHORESIS; ELECTROSPRAY IONIZATION; ENZYME IMMOBILIZATION; FLUORINE CONTAINING POLYMERS; MASS SPECTROMETRY; NUCLEOTIDES; SEPARATION;

ALDEHYDE DEHYDROGENASE; DE NOVO SEQUENCING; MALATE DEHYDROGENASE; NEGATIVE STAIN; SORBITOL DEHYDROGENASE;

ENZYME ACTIVITY;

ALDEHYDE DEHYDROGENASE; FLUORIDE; IDITOL DEHYDROGENASE; MALATE DEHYDROGENASE; OXIDOREDUCTASE; POLYVINYLIDENE DIFLUORIDE; RESIN;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME ISOLATION; FEMALE; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; STAINING; TANDEM MASS SPECTROMETRY; TWO DIMENSIONAL ELECTROPHORESIS;

ANIMALS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENZYME ACTIVATION; ENZYMES, IMMOBILIZED; LIVER; MICE; OXIDOREDUCTASES; POLYVINYLS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 43649108590     PISSN: 00032670     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.aca.2008.04.046     Document Type: Article

References (13)

1. Sharma R., Chisti Y., and Banerjee U.C. Biotechnol. Adv. 19 (2001) 627
2. Shimazaki Y., and Sugawara Y. Anal. Biochem. 328 (2004) 87
3. Shimazaki Y., Sugawara Y., and Manabe T. Proteomics 4 (2004) 1406
4. Shimazaki Y., Hiraka Y., Uesugi M., and Manabe T. Biochem. Biophys. Acta 1696 (2004) 51
5. Shimazaki Y. J. Biochem. Biophys. Methods 70 (2007) 487
6. Roig M.G., Bello J.F., Moreno de Vega M.A., Cachaza J.M., and Kennedy J.F. J. Chem. Technol. Biotechnol. 49 (1990) 99
7. Dawson R.M.C., Elliot D.C., Elliot W.H., and Jones K.M. In: Dawson R.M.C., et al. (Ed). Data for Biochemical Research (1986), Oxford University Press, New York 122
8. Kuramitsu S., and Hamaguchi K. In: Yamakawa T., et al. (Ed). Seikagaku Data Book I (1979), Tokyo kagaku Dojin, Tokyo 92
9. Fenn J.B., Mann M., Meng C.K., Wong S.F., and Whitehouse C.M. Science 246 (1989) 64
10. Henzel W.J., Billeci T.M., Stults J.T., Wong S.C., Grimley C., and Watanabe C. Proc. Natl. Acad. Sci. 90 (1993) 5011
11. Mann M., and Wilm M. Anal. Chem. 66 (1994) 4390
12. Corthals G.L., Gygi S.P., Aebersold R., and Patterson S.D. In: Rabilloud T. (Ed). Proteome Research (2000), Springer-Verlag, Berlin Heidelberg 197
13. Shimazaki Y., and Watanabe S. Clin. Chim. Acta 390 (2008) 145