Analysis of lipid hydrolytic activity by esterase on blotting membrane followed by separation using non-denaturing two-dimensional gel electrophoresis

Biotechnology and Bioengineering

Volumn 98, Issue 4, 2007, Pages 732-736

  Shimazaki, Youji ^a   Miyamoto, Masayuki ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Carboxylesterase; Direct blue 71; HDL; Phosphatidylcholine

Indexed keywords

CELL MEMBRANES; ELECTROPHORESIS; ENZYME ACTIVITY; ENZYME IMMOBILIZATION;

CARBOXYLESTERASE; DIRECT BLUE 71; PHOSPHATIDYLCHOLINE;

LIPIDS;

CARBOXYLESTERASE; HIGH DENSITY LIPOPROTEIN; PHOSPHATIDYLCHOLINE;

ARTICLE; CONTROLLED STUDY; ELECTROBLOTTING; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; GEL ELECTROPHORESIS; HUMAN; HYDROLYSIS; LIPID ANALYSIS; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; SEPARATION TECHNIQUE;

ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENZYME ACTIVATION; ENZYMES, IMMOBILIZED; ESTERASES; HYDROLYSIS; LIPIDS; LIPOPROTEINS; MEMBRANES, ARTIFICIAL; PROTEIN DENATURATION;

EID: 35649024331     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21437     Document Type: Article

References (21)

1. Chellan B, Appukuttan PS, Jayakumari N. 2006. Electroelution of lipoprotein (a)[Lp(a)] from native polyacrylamide gels: A new, simple method to purify Lp(a). J Biochem Biophys Methods 31:43-53.
2. Ernes AV, Latner AL, Martin JA. 1975. Electrofocusing followed by gradient electrophoresis: A two-dimensional polyacrylamide geltechnique for the separation of proteins and its application to the immunoglobulin. Clin Chim Acta 64:69-78.
3. Felgenhauar K, Hagedorn D. 1980. Two-dimensional separation of human body fluid proteins. Clin Chim Acta 100:121-132.
4. Hong H, Yoo G, Choi J. 2000. Direct blue 71 staining of proteins bound to blotting membranes. Electrophoresis 21:841-845.
5. Hui D, Howles P. 2002. Carboxyl ester lipase: Structure-function relationship and physiological role in lipoprotein metabolism and atherosclerosis. J Lipid Res 43:2017-2030.
6. Johnston KJ, Ashford AE. 1980. A simultaneous-coupling azo dye method for the quantitative assay of esterase using α-naphthyl acetate as substrate. Histochem J 12:221-234.
7. Kuramitsu S, Hamaguchi K. 1979. Protein constants. In: Yamakawa T., Imabori K, Ui N, Osawa T, Kagawa Y, Kamishiro T, Nakagawa H, Narita K, Nishinaka Y, Nishimura N, Nojima S, Yamishima I. editors. Seikagaku data book I. Tokyo: Tokyo kagaku Dojin. p 91-135.
8. Macfarlane R, Bondarenko P, Cockrill S, Cruzado I, Koss W, McNeal C, Spiekerman A, Watkins L. 1997. Development of a lipoprotein profile using capillary electrophoresis and mass spectrometry. Electrophoresis 18:1796-1806.
9. Manabe T, Tachi K, Kojima K, Okuyama K. 1979. Two-dimensional electrophoresis of plasma proteins without denaturing agents. J Biochem 85:649-659.
10. Manabe T, Takahashi Y, Okuyama T. 1984. An electroblotting apparatus for multiple replica technique and identification of human serum proteins on micro two-dimensional gels. Anal Biochem 143:39-45.
11. Manabe T, Takahashi Y, Higuchi N, Okuyama T. 1985. Identification map of human plasma proteins: Micro two-dimensional electrophoresis followed by multiple immunoreplica technique. Electrophoresis 6:462-467.
12. Morgan EW, Yan B, Greenway D, Petersen DR, Parkinson A. 1994. Purification and characterization of two rat liver microsomal carboxylesterase (Hydrolase A and B). Arch Biochem Biophys 315:495-512.
13. Ohlsson BG, Weström BR, Karlsson BW. 1986. Enzymoblotting: A method for localizing protainases and their zymogens using para-nitroanilide substrates after agarose gel electrophoresis and transfer to nitrocellulose. Anal Biochem 152:239-244.
14. Poole MA, Fischl AS, Carman GM. 1985. Enzymatic detection of phospholipid biosynthetic enzymes after electroblotting. J Bacteriol 161:772-774.
15. Robern H. 1982. The application of sodium deoxycholate and sephacryl-200 for the delipidation and separation of high density lipoprotein. Experimentia 38:437-439.
16. Shackelford DA, Zivin JA. 1993. Renaturation of calcium/calmodulin- dependent protein kinase activity after electrophoretic transfer from sodium dodecyl sulfate-polyacrylamide gels to membranes. Anal Biochem 211:131-138.
17. Shimazaki Y, Manabe T. 2005. Detection of activity and mass spectrometric identification of mouse liver carboxylesterase and aldehyde dehydrogenase separated by non-denaturing two-dimensional electrophoresis after extraction with detergents. Biochem Biophys Acta 1749:95-101.
18. Shimazaki Y, Sugawara Y, Ohtsuka Y, Manabe T. 2003. Analysis of the activity and identification of enzymes after separation of cytosol proteins in mouse liver by microscale nondenaturing two-dimensional electrophoresis. Proteomics 3:2002-2007.
19. Shimazaki Y, Sugawara Y, Manabe T. 2004. Nondenaturing two-dimensional electrophoresis enzyme profile involving activity and sequence structure of cytosol proteins from mouse liver. Proteomics 4:1406-1411.
20. Shimazaki Y, Kishi H, Mori M, Yasuda C, Manabe T. 2006. Analysis of hydrolytic activity of phospholipase Cα from porcine retina on retinylester and phosphatidylcholine using non-denaturing two-dimensional electrophoresis and mass spectrometry. J Chromatogr B 843:42-46.
21. Zschörnig O, Pietsch M, Süß R, Schiller J, Gütschow M. 2005. Cholesterol esterase action on human high density lipoproteins and inhibition studies: Detection by MALDI-TOF MS. J Lipid Res 46:803-811.