The nuclear translocation assay for intracellular protein-protein interactions and its application to the Bcr coiled-coil domain

BioTechniques

Volumn 49, Issue 1, 2010, Pages 519-524

  Dixon, Andrew S ^a   Lim, Carol S ^a  

^a UNIVERSITY OF UTAH   (United States)

Author keywords

Bcr; DsRed; EGFP; Nuclear translocation assay; Protein switch; Protein protein interactions

Indexed keywords

BREAKPOINT CLUSTER REGION PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN; RED FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PHOTOPROTEIN; PROTEIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GENE AMPLIFICATION; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; ANIMAL; CELL LINE; CELL NUCLEUS; CELL STRAIN COS1; CERCOPITHECUS; GENE EXPRESSION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PROTEIN ANALYSIS; PROTEIN TRANSPORT;

ANIMALS; CELL LINE; CELL NUCLEUS; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; COS CELLS; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; PROTEIN INTERACTION MAPPING; PROTEIN TRANSPORT; PROTEINS; RECOMBINANT FUSION PROTEINS;

EID: 77957932377     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113452     Document Type: Article

References (28)

1. Stein, A., R.A. Pache, P. Bernado, M. Pons, and P. Aloy. 2009. Dynamic interactions of proteins in complex networks: a more structured view. FEBS J. 276:5390-5405.
2. Davis, J.R., M. Kakar, and C.S. Lim. 2007. Controlling protein compartmentalization to overcome disease. Pharm. Res. 24:17-27.
3. Tsukaguchi, H., H. Matsubara, S. Taketani, Y. Mori, T. Seido, and M. Inada. 1995. Binding-, intracellular transport-, and biosynthesis-defective mutants of vasopressin type 2 receptor in patients with X-linked nephrogenic diabetes insipidus. J. Clin. Invest. 96:2043-2050.
4. Buchanan, P.J., R.K. Ernst, J.S. Elborn, and B. Schock. 2009. Role of CFTR, Pseudomonas aeruginosa and Toll-like receptors in cystic fibrosis lung inflammation. Biochem. Soc. Trans. 37:863-867.
5. Zhong, N. and K.H. Weisgraber. 2009. Understanding the basis for the association of apoE4 with Alzheimer's disease: opening the door for therapeutic approaches. Curr. Alzheimer Res. 6:415-418.
6. Gloerich, J., R.A. Wevers, J.A. Smeitink, B.G. van Engelen, and L.P. van den Heuvel. 2007. Proteomics approaches to study genetic and metabolic disorders. J. Proteome Res. 6:506-512. (Pubitemid 46340154)
7. Pfleger, K.D. and K.A. Eidne. 2006. Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET). Nat. Methods 3:165-174.
8. Miyawaki, A., J. Llopis, R. Heim, J.M. McCaffery, J.A. Adams, M. Ikura, and R.Y. Tsien. 1997. Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388:882-887. (Pubitemid 27377061)
9. Suter, B., S. Kittanakom, and I. Stagljar. 2008. Two-hybrid technologies in proteomics research. Curr. Opin. Biotechnol. 19:316-323.
10. Johnsson, N. and A. Varshavsky. 1994. Split ubiquitin as a sensor of protein interactions in vivo. Proc. Natl. Acad. Sci. USA 91:10340-10344.
11. Kakar, M., A.B. Cadwallader, J.R. Davis, and C.S. Lim. 2007. Signal sequences for targeting of gene therapy products to subcellular compartments: the role of CRM1 in nucleocytoplasmic shuttling of the protein switch. Pharm. Res. 24:2146-2155.
12. Kakar, M., J.R. Davis, S.E. Kern, and C.S. Lim. 2007. Optimizing the protein switch: altering nuclear import and export signals, and ligand binding domain. J. Control. Release 120:220-232. (Pubitemid 47058637)
13. Htun, H., J. Barsony, I. Renyi, D.L. Gould, and G.L. Hager. 1996. Visualization of glucocorticoid receptor translocation and intranuclear organization in living cells with a green fluorescent protein chimera. Proc. Natl. Acad. Sci. USA 93:4845-4850.
14. Taylor, C.M. and A.E. Keating. 2005. Orientation and oligomerization specificity of the Bcr coiled-coil oligomerization domain. Biochemistry 44:16246-16256.
15. Rowley, J.D. 1973. Letter: a new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining. Nature 243:290-293.
16. Sandberg, A.A., R.M. Gemmill, B.K. Hecht, and F. Hecht. 1986. The Philadelphia chromosome: a model of cancer and molecular cytogenetics. Cancer Genet. Cytogenet. 21:129-146.
17. Zhang, X., R. Subrahmanyam, R. Wong, A.W. Gross, and R. Ren. 2001. The NH(2)-terminal coiled-coil domain and tyrosine 177 play important roles in induction of a myeloproliferative disease in mice by Bcr-Abl. Mol. Cell. Biol. 21:840-853.
18. Guo, X.Y., J.M. Cuillerot, T. Wang, Y. Wu, R. Arlinghaus, D. Claxton, C. Bachier, J. Greenberger, et al. 1998. Peptide containing the BCR oligomerization domain (AA 1-160) reverses the transformed phenotype of p210bcr-abl positive 32D myeloid leukemia cells. Oncogene 17:825-833.
19. Beissert, T., A. Hundertmark, V. Kaburova, L. Travaglini, A.A. Mian, C. Nervi, and M. Ruthardt. 2008. Targeting of the N-terminal coiled coil oligomerization interface by a helix-2 peptide inhibits unmutated and imatinib-resistant BCR/ABL. Int. J. Cancer 122:2744-2752. (Pubitemid 351631376)
20. Beissert, T., E. Puccetti, A. Bianchini, S. Guller, S. Boehrer, D. Hoelzer, O.G. Ottmann, C. Nervi, and M. Ruthardt. 2003. Targeting of the N-terminal coiled coil oligomerization interface of BCR interferes with the transformation potential of BCR-ABL and increases sensitivity to STI571. Blood 102:2985-2993. (Pubitemid 37248874)
21. Huang, S.F., D.B. Liu, J.M. Zeng, Q. Xiao, M. Luo, W.P. Zhang, K. Tao, J.P. Wen, et al. 2009. Cloning, expression, purification and functional characterization of the oligomerization domain of Bcr-Abl oncoprotein fused to the cytoplasmic transduction peptide. Protein Expr. Purif. 64:167-178.
22. Kanwal, C., H. Li, and C.S. Lim. 2002. Model system to study classical nuclear export signals. AAPS PharmSci 4:E18. (Pubitemid 35455114)
23. Kanwal, C., S. Mu, S.E. Kern, and C.S. Lim. 2004. Bidirectional on/off switch for controlled targeting of proteins to subcellular compartments. J. Control. Release 98:379-393. (Pubitemid 39093949)
24. Li, H., M.L. Fidler, and C.S. Lim. 2005. Effect of initial subcellular localization of progesterone receptor on import kinetics and transcriptional activity. Mol. Pharm. 2:509-518.
25. Li, H., G. Yan, S.E. Kern, and C.S. Lim. 2003. Correlation among agonist dose, rate of import, and transcriptional activity of liganded progesterone receptor B isoform in living cells. Pharm. Res. 20:1574-1580.
26. Lim, C.S., C.T. Baumann, H. Htun, W. Xian, M. Irie, C.L. Smith, and G.L. Hager. 1999. Differential localization and activity of the A- and B-forms of the human progesterone receptor using green fluorescent protein chimeras. Mol. Endocrinol. 13:366-375.
27. Grefen, C., K. Stadele, K. Ruzicka, P. Obrdlik, K. Harter, and J. Horak. 2008. Subcellular localization and in vivo interactions of the Arabidopsis thaliana ethylene receptor family members. Mol. Plant. 1:308-320.
28. Knauer, S.K. and R.H. Stauber. 2005. Development of an autofluorescent translocation biosensor system to investigate proteinprotein interactions in living cells. Anal. Chem. 77:4815-4820.