메뉴 건너뛰기
Bioorganic and Medicinal Chemistry Letters
Volumn 20, Issue 21, 2010, Pages 6199-6202
Design, synthesis and activity of benzothiazole-based inhibitors of NO production in LPS-activated macrophages
Author keywords

Benzothiazole; Inhibitor; Nitric oxide; NOS
Indexed keywords

4 AMINOBENZYLOXYBENZOTHIAZOLE DERIVATIVE; 4 BENZYLOXYBENZOTHIAZOLE DERIVATIVE; BENZOTHIAZOLE DERIVATIVE; CYCLOOXYGENASE 2; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INDUCIBLE NITRIC OXIDE SYNTHASE; LIPOPOLYSACCHARIDE; MESSENGER RNA; NITRIC OXIDE; UNCLASSIFIED DRUG;
EID: 77957862493     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2010.08.112     Document Type: Article
Times cited : (17)
References (32)
  • 30
    • 77957869231 scopus 로고    scopus 로고
    • 50 values
    • 50 values.
  • 31
    • 77957876231 scopus 로고    scopus 로고
    • 6 cells/60 mm dish) were stimulated with LPS (1 μg/mL) in the presence or absence of test compounds. After incubation for 20 h, the cells were washed and lyzed with lysis buffer. Twenty microgram protein of cell lysates was applied on 8% SDS-polyacrylamide gels and transferred to PVDF membrane by a standard method. The membrane was probed with antibody for anti-mouse iNOS (Transduction Laboratories, Lexington, KY) and anti-actin (Sigma, St. Louis, MO). The bands were visualized using an enhanced chemiluminescence (ECL) detection kit (Amersham Bioscience, Piscataway, NJ) according to the manufacturer's instruction
    • 6 cells/60 mm dish) were stimulated with LPS (1 μg/mL) in the presence or absence of test compounds. After incubation for 20 h, the cells were washed and lyzed with lysis buffer. Twenty microgram protein of cell lysates was applied on 8% SDS-polyacrylamide gels and transferred to PVDF membrane by a standard method. The membrane was probed with antibody for anti-mouse iNOS (Transduction Laboratories, Lexington, KY) and anti-actin (Sigma, St. Louis, MO). The bands were visualized using an enhanced chemiluminescence (ECL) detection kit (Amersham Bioscience, Piscataway, NJ) according to the manufacturer's instruction.
  • 32
    • 77957886627 scopus 로고    scopus 로고
    • note
    • 6 cells/60 mm dish) were stimulated for 6 h with LPS (1 μg/mL) in the presence or absence of test compounds. After washing twice with phosphate buffered saline, total RNA was isolated from cell pellet, using an RNA isolation reagent (Trizol, Invitrogen, Carlsbad, CA). Two microgram of RNA was reverse transcribed into cDNA using reverse transcriptase and random hexamer. The PCR samples, contained in the reaction mixture, were comprised of mixture buffer, dNTP, Taq DNA polymerase (Promega, Madison, WI) and primers (sense and antisense). The sense and antisense primers for iNOS were 5′-ATGTCCGAAGCAAACATCAC-3′ and 5′-TAATGTCCAGGAAGTAGGTG-3′, respectively. The sense and antisense primers for β-actin were 5′-TGTGATGGTGGGAATGGGTCAG-3′ and 5′-TTTGATGTCACGCACGATTTCC-3′, respectively. The PCR amplification was performed under following conditions; 25 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s and extension at 72 °C for 30 s, using thermal cycler (Gene Amp PCR system 2400, Applied Biosystems, Foster City, CA). The amplified PCR products were separated on a 2% agarose gel.

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.