Adipocytes decrease Runx2 expression in osteoblastic cells: Roles of PPARγ and adiponectin

Journal of Cellular Physiology

Volumn 225, Issue 3, 2010, Pages 837-845

  Liu, Li Fen ^a,b   Shen, Wen Jun ^a,b   Zhang, Zhong Hua ^a,b   Wang, Li Juan ^b   Kraemer, Fredric B ^a,b  

^a STANFORD UNIVERSITY   (United States)

^b VA PALO ALTO HEALTH CARE SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADIPONECTIN; ADIPONECTIN RECEPTOR 1; ALKALINE PHOSPHATASE; COLLAGEN TYPE 1; COLLAGEN TYPE 2; LIPOCORTIN 5; MESSENGER RNA; OSTEOCALCIN; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; PROPIDIUM IODIDE; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR RUNX2; ADIPONECTIN RECEPTOR; ADIPONECTIN RECEPTOR 1, MOUSE; ADIPONECTIN, MOUSE; PLASMINOGEN ACTIVATOR INHIBITOR 1; RUNX2 PROTEIN, MOUSE;

ADIPOCYTE; ANIMAL CELL; ARTICLE; BONE DEVELOPMENT; BONE MARROW CELL; CELL DIFFERENTIATION; CELL FUNCTION; CONTROLLED STUDY; ENZYME ACTIVITY; EX VIVO STUDY; GENE EXPRESSION; MALE; MOUSE; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RECEPTOR DOWN REGULATION; STAINING; ADIPOGENESIS; ANIMAL; APOPTOSIS; C57BL MOUSE; CELL STRAIN 3T3; COCULTURE; CULTURE MEDIUM; DOWN REGULATION; GENETIC TRANSFECTION; GENETICS; METABOLISM; PARACRINE SIGNALING; RNA INTERFERENCE;

3T3-L1 CELLS; ADIPOCYTES; ADIPOGENESIS; ADIPONECTIN; ALKALINE PHOSPHATASE; ANIMALS; APOPTOSIS; COCULTURE TECHNIQUES; CORE BINDING FACTOR ALPHA 1 SUBUNIT; CULTURE MEDIA, CONDITIONED; DOWN-REGULATION; MALE; MICE; MICE, INBRED C57BL; OSTEOBLASTS; OSTEOGENESIS; PARACRINE COMMUNICATION; PLASMINOGEN ACTIVATOR INHIBITOR 1; PPAR GAMMA; PROMOTER REGIONS, GENETIC; RECEPTORS, ADIPONECTIN; RNA INTERFERENCE; RNA, MESSENGER; TRANSFECTION;

EID: 77957740180     PISSN: 00219541     EISSN: 10974652     Source Type: Journal    
DOI: 10.1002/jcp.22291     Document Type: Article

References (39)

1. Akune T, Ohba S, Kamekura S, Yamaguchi M, Chung UI, Kubota N, Terauchi Y, Harada Y, Azuma Y, Nakamura K, Kadowaki T, Kawaguchi H. 2004. PPARgamma insufficiency enhances osteogenesis through osteoblast formation from bone marrow progenitors. J Clin Invest 113: 846-855.
2. Alessi MC, Poggi M, Juhan-Vague I. 2007. Plasminogen activator inhibitor-1, adipose tissue and insulin resistance. Curr Opin Lipidol 18: 240-245.
3. Ali AA, Weinstein RS, Stewart SA, Parfitt AM, Manolagas SC, Jilka RL. 2005. Rosiglitazone causes bone loss in mice by suppressing osteoblast differentiation and bone formation. Endocrinology 146: 1226-1235.
4. Beresford JN, Bennett JH, Devlin C, Leboy PS, Owen ME. 1992. Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures. J Cell Sci 102: 341-351.
5. Berner HS, Lyngstadaas SP, Spahr A, Monjo M, Thommesen L, Drevon CA, Syversen U, Reseland JE. 2004. Adiponectin and its receptors are expressed in bone-forming cells. Bone 35: 842-849.
6. Cao JJ, Gregoire BR, Gao H. 2009. High-fat diet decreases cancellous bone mass but has no effect on cortical bone mass in the tibia in mice. Bone 44: 1097-1104.
7. Chamberlain G, Fox J, Ashton B, Middleton J. 2007. Concise review: Mesenchymal stem cells: Their phenotype, differentiation capacity, immunological features, and potential for homing. Stem Cells 25: 2739-2749.
8. Duque G. 2008. Bone and fat connection in aging bone. Curr Opin Rheumatol 20: 429-434.
9. Elbaz A, Wu X, Rivas D, Gimble JM, Duque G. 2010. Inhibition of fatty acid biosynthesis prevents adipocyte lipotoxicity on human osteoblasts in vitro. J Cell Mol Med 14: 982-991.
10. Fowlkes JL, Bunn RC, Liu L, Wahl EC, Coleman HN, Cockrell GE, Perrien DS, Lumpkin CK, Jr., Thrailkill KM. 2008. Runt-related transcription factor 2 (RUNX2) and RUNX2-related osteogenic genes are down-regulated throughout osteogenesis in type 1 diabetes mellitus. Endocrinology 149: 1697-1704.
11. Gimble JM, Dorheim MA, Cheng Q, Medina K, Wang CS, Jones R, Koren E, Pietrangeli C, Kincade PW. 1990. Adipogenesis in a murine bone marrow stromal cell line capable of supporting B lineage lymphocyte growth and proliferation: Biochemical and molecular characterization. Eur J Immunol 20: 379-387.
12. Gimble JM, Robinson CE, Wu X, Kelly KA. 1996. The function of adipocytes in the bone marrow stroma: An update. Bone 19: 421-428.
13. Kahn SE, Zinman B, Lachin JM, Haffner SM, Herman WH, Holman RR, Kravitz BG, Yu D, Heise MA, Aftring RP, Viberti G. 2008. Rosiglitazone-associated fractures in type 2 diabetes: An Analysis from A Diabetes Outcome Progression Trial (ADOPT). Diabetes Care 31: 845-851.
14. Kanazawa I, Yamaguchi T, Yano S, Yamauchi M, Yamamoto M, Sugimoto T. 2007. Adiponectin and AMP kinase activator stimulate proliferation, differentiation, and mineralization of osteoblastic MC3T3-E1 cells. BMC Cell Biol 8: 51.
15. Kawaguchi H. 2004. Ageing and bone. Clin Calcium 14: 275-283.
16. Kawaguchi H, Akune T, Yamaguchi M, Ohba S, Ogata N, Chung UI, Kubota N, Terauchi Y, Kadowaki T, Nakamura K. 2005. Distinct effects of PPARgamma insufficiency on bone marrow cells, osteoblasts, and osteoclastic cells. J Bone Miner Metab 23: 275-279.
17. Kershaw EE, Flier JS. 2004. Adipose tissue as an endocrine organ. J Clin Endocrinol Metab 89: 2548-2556.
18. Komori T. 2005. Regulation of skeletal development by the Runx family of transcription factors. J Cell Biochem 95: 445-453.
19. Komori T. 2006. Regulation of osteoblast differentiation by transcription factors. J Cell Biochem 99: 1233-1239.
20. Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, Deguchi K, Shimizu Y, Bronson RT, Gao YH, Inada M, Sato M, Okamoto R, Kitamura Y, Yoshiki S, Kishimoto T. 1997. Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 89: 755-764.
21. Lee NK, Sowa H, Hinoi E, Ferron M, Ahn JD, Confavreux C, Dacquin R, Mee PJ, McKee MD, Jung DY, Zhang Z, Kim JK, Mauvais-Jarvis F, Ducy P, Karsenty G. 2007. Endocrine regulation of energy metabolism by the skeleton. Cell 130: 456-469.
22. Lee WY, Rhee EJ, Oh KW, Kim SY, Jung CH, Yun EJ, Baek KH, Kang MI, Kim SW. 2006. Identification of adiponectin and its receptors in human osteoblast-like cells and association of T45G polymorphism in exon 2 of adiponectin gene with lumbar spine bone mineral density in Korean women. Clin Endocrinol (Oxf) 65: 631-637.
23. Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402-408.
24. Luo XH, Guo LJ, Xie H, Yuan LQ, Wu XP, Zhou HD, Liao EY. 2006. Adiponectin stimulates RANKL and inhibits OPG expression in human osteoblasts through the MAPK signaling pathway. J Bone Miner Res 21: 1648-1656.
25. Maurin AC, Chavassieux PM, Frappart L, Delmas PD, Serre CM, Meunier PJ. 2000. Influence of mature adipocytes on osteoblast proliferation in human primary cocultures. Bone 26: 485-489.
26. Moerman EJ, Teng K, Lipschitz DA, Lecka-Czernik B. 2004. Aging activates adipogenic and suppresses osteogenic programs in mesenchymal marrow stroma/stem cells: The role of PPAR-gamma2 transcription factor and TGF-beta/BMP signaling pathways. Aging Cell 3: 379-389.
27. Nuttall ME, Gimble JM. 2004. Controlling the balance between osteoblastogenesis and adipogenesis and the consequent therapeutic implications. Curr Opin Pharmacol 4: 290-294.
28. Oshima K, Nampei A, Matsuda M, Iwaki M, Fukuhara A, Hashimoto J, Yoshikawa H, Shimomura I. 2005. Adiponectin increases bone mass by suppressing osteoclast and activating osteoblast. Biochem Biophys Res Commun 331: 520-526.
29. Owen M. 1988. Marrow stromal stem cells. J Cell Sci Suppl 10: 63-76.
30. Pietrangeli CE, Hayashi S, Kincade PW. 1988. Stromal cell lines which support lymphocyte growth: Characterization, sensitivity to radiation and responsiveness to growth factors. Eur J Immunol 18: 863-872.
31. Ruan H, Zarnowski MJ, Cushman SW, Lodish HF. 2003. Standard isolation of primary adipose cells from mouse epididymal fat pads induces inflammatory mediators and down-regulates adipocyte genes. J Biol Chem 278: 47585-47593.
32. Schwartz AV, Sellmeyer DE, Vittinghoff E, Palermo L, Lecka-Czernik B, Feingold KR, Strotmeyer ES, Resnick HE, Carbone L, Beamer BA, Park SW, Lane NE, Harris TB, Cummings SR. 2006. Thiazolidinedione use and bone loss in older diabetic adults. J Clin Endocrinol Metab 91: 3349-3354.
33. Shinoda Y, Yamaguchi M, Ogata N, Akune T, Kubota N, Yamauchi T, Terauchi Y, Kadowaki T, Takeuchi Y, Fukumoto S, Ikeda T, Hoshi K, Chung UI, Nakamura K, Kawaguchi H. 2006. Regulation of bone formation by adiponectin through autocrine/paracrine and endocrine pathways. J Cell Biochem 99: 196-208.
34. Shockley KR, Lazarenko OP, Czernik PJ, Rosen CJ, Churchill GA, Lecka-Czernik B. 2009. PPARgamma2 nuclear receptor controls multiple regulatory pathways of osteoblast differentiation from marrow mesenchymal stem cells. J Cell Biochem 106: 232-246.
35. Tilg H, Moschen AR. 2006. Adipocytokines: Mediators linking adipose tissue, inflammation and immunity. Nat Rev Immunol 6: 772-783.
36. Wang D, Christensen K, Chawla K, Xiao G, Krebsbach PH, Franceschi RT. 1999. Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. J Bone Miner Res 14: 893-903.
37. Williams GA, Wang Y, Callon KE, Watson M, Lin JM, Lam JB, Costa JL, Orpe A, Broom N, Naot D, Reid IR, Cornish J. 2009. In vitro and in vivo effects of adiponectin on bone. Endocrinology 150: 3603-3610.
38. Wolf G. 2008. Energy regulation by the skeleton. Nutr Rev 66: 229-233.
39. Yokota T, Meka CS, Medina KL, Igarashi H, Comp PC, Takahashi M, Nishida M, Oritani K, Miyagawa J, Funahashi T, Tomiyama Y, Matsuzawa Y, Kincade PW. 2002. Paracrine regulation of fat cell formation in bone marrow cultures via adiponectin and prostaglandins. J Clin Invest 109: 1303-1310.