Rauvolfia serpentina (L). benth. ex kurz.-A review

Asian Journal of Plant Sciences

Volumn 9, Issue 6, 2010, Pages 285-298

  Dey, Abhijit ^a   De, J N ^b  

^a PRESIDENCY UNIVERSITY   (India)

^b Charuchandra College   (India)

Author keywords

Rauvolfia serpentina; Sarpagandha; Snakebites

Indexed keywords

APOCYNACEAE; RAUVOLFIA SERPENTINA;

EID: 77957714866     PISSN: 16823974     EISSN: 18125697     Source Type: Journal    
DOI: 10.3923/ajps.2010.285.298     Document Type: Review

References (232)

1. Abdel-Moty, S.G., S. Sakai, N Aimi, H. Takayama and M. Kitajima et al., 1998. Synthesis of cytotoxic 1 -polyhydroxyalkyl-β-carboline derivatives. Eur. J. Med. Chem., 32: 1009-1017.
2. Agbalyam, S.G., 1961. Synthetic analogs of reserpine. Usp. Khi.,30: 1175-1195.
3. Ahmad, S., M.N. Amin, M.A.K. Azad and M.A. Mosaddik, 2002. Micropropagation and plant regeneration of Rauvolfia serpentina by tissue culture technique. Pak. J. Biol. Sci., 5: 75-79.
4. Akram, M. and I. Ilahi, 1985a. Biosynthesis of Some Important Alkaloids by Stem Cultures of Rauwolfia serpentina Benth. In: Plant Tissue Culture, Ilahi, I. (Eds.). University of Peshawar, Pakistan, pp: 44-45.
5. Akram, M. and I. Ilahi, 1985b. Bud Differentiation in Root Callus of Regenerated Plantlets of Raimolfia serpentina. In: Plant Tissue Culture, Qureshi, A. and I. John (Eds.). PARC, Islamabad, pp: 18-23.
6. Akram, M. and I. Ilahi, 1986. Plantlets formation in root callus of Raimolfia serpentina. Pak. J. Bot, 18: 15-19.
7. Ammal Janaki, E.L., 1962. Tetraploidy in Rauvolfia serpentina benth. Curr. Sci., 31: 520-524.
8. Andreev, I.O., K.V. Spiridonova, V.T. Solovyan and V.A. Kunakh, 2005. Variability of nbosomal RNA genes in Rauwolfia species: Parallelism between tissue culture-induced rearrangements and interspecies polymorphism. Cell Biol. Int., 29: 21-27.
9. Anisuzzaman, M., A.H.M.M. Rahman, M. Harun-Or-Rashid, A.T.M. Naderuzzaman and A.K.M.R. Islam, 2007. An ethnobotanical study of madhupur, Tangail. J. Applied Sci. Res., 3: 519-530.
10. Anitha, S. and B.D.R. Kumari, 2006. Stimulation of reserpine biosynthesis in the callus of Rauvolfia tetraphyla L. by precursor feeding. Afr. J. Biotechnol, 5: 659-661.
11. Ansari, A.A., 1993. Threatened medicinal plants from Madhaulia forest of Gorakhpur. J. Econ Bot. Taxonomy, 17: 241-241.
12. Arend, J., H. Warzecha and J. Stockigt, 2000. Hydroquinone: 0-glucosyltransferase from cultivated Rauvolfia cells: Enrichment and partial amino acid sequences. Phytochemistry, 53: 187-193.
13. Azeem, S.W., M.A. Khan and I. Ahmad, 2005. Characterisation of oxidation products of Rauwolfia alkaloids. Pak. J. Pharm. Sci., 18: 33-35.
14. Badhwar, R.L., G.V. Karira and S. Ramaswami, 1955. Rauvolfia serpentina: The wonder drug of India (Rauvolfia sarpagandha). Ind. Forester, 81: 258-268.
15. Badhwar, R.L., G.V. Karira and S. Ramaswami, 1956. Methods of propagation and their effect on root production in Rauwolfia serpentina. Ind. J. Pharm., 18: 170-175.
16. Bailey, P.D., K.M. Morgan, D.I. Smith and J.M. Vernon, 2000. New asymmetric routes to ajmaline and suaveoline indole alkaloids. Perkin Trans. J. Chem. Soc.,1: 3566-3577.
17. Baksha, R., M.A.A. Jahan, R. Khatun and J.L. Munshi, 2007. In vitro rapid clonal propagation of Rauvolfia serpentina (Linn.) benth. Bangladesh J. Sci. Indus. Res., 42: 37-44.
18. Banerjee, N. and A.K. Sharma, 1989. Chromosome constitution and alkaloid content in Rauwolfiia L. (Apocynaceae). Cytologia, 54: 723-728.
19. Baruah, A. and S.C. Nath, 2000. Diploid autecological and ploidal leaf epidermal characters in Rauvolfia serpentina benth. with emphasis to ecophysiological adaptability. Adv. Plant Sci., 13: 245-251.
20. Bedi, Y.S., 1990. Cytology of hardwoods. Proc. Plant Sci., 100: 91-95.
21. Benjamin, B.D., G. Rqja and M.R. Heble, 1993. Agrobacterium rhizogenes mediated tranformation of Rauvolfia serpentina: Regeneration and alkaloid synthesis. Plant Cell Tissue Organ Cult., 35: 253-257.
22. Bertler, A., 1961. Effect of reserpine on the storage of catechol amines in brain and other tissues. Acta Physiol. Scand, 51: 75-83.
23. Bhaduri, P.N. and P.K. Biswas, 1965. Increasing root yield of Rauvolfia serpentina by colchiploidy. Sci. Cult, 31: 197-200.
24. Bharadwaj, K.R., 1988. Quantitative estimation of rutin in Rauvolfia serpentina benth. ex Kurz. Curr. Sci., 57: 439-439.
25. Bhatara, V.S., J.N. Sharma, S. Gupta and Y.K Gupta, 1997. Images in psychiatry. Rauwolfia serpentina: The first antipsychotic. Am. J. Psychiatry, 154: 894-894.
26. Bhatt, R., M. Anf, A.K. Gaur and P.B. Rao, 2008. Rauwolfia serpentina: Protocol optimization for in vitro propagation. Afr. J. Biotechnol., 7: 4265-4268.
27. Bhattacharjee, S.K. and P.N Bhaduri, 1959. Floral biology and cytogenetics of diploid and autopolyploid of R. serpentina andi?. canescens. Bull. Bot. Soc. Beng., 13: 86-92.
28. Bhattacharyya, P.K. and K. Sarkar, 1998. Flora of west champaran district, Bihar. Botanical Survey of India, Calcutta, pp: 534. http://kbd.kew.org/kbd/detailedresult.do?id=334984.
29. Biswas, K., 1956. Cultivation of Rauvolfia in West Bengal. Ind. J. Pharm., 18: 170-175.
30. Bose, S., 1954. Rauwolfine: A new alkaloid oiRauwolfia serpentina benth. J. Ind. Chem. Soc, 1: 47-47.
31. Bracher, D. and T.M. Kutchan, 1992a. Polymerase chain reaction comparison of the gene for Strictosidine synthase from tenRauvolfia species. Plant Cell Rep., 11: 179-182.
32. Bracher, D. and T.M. Kutchan, 1992b. Strictosidine synthase from Rauvolfia serpentina: Analysis of a gene involved in indole alkaloid biosynthesis. Arch. Biochem. Biophys., 294: 717-723.
33. Brodie, B.B., K.F. Finer, F.B. Orlans, G.F. Quinn and F. Sulser, 1960. Evidence that tranquilizing action of reserpine is associated with change in brain serotonin. J. Pharmacol. Exp. Ther., 129: 250-256.
34. Chandra, V., 1954. Vegetative propagation of Rauwolfia serpentina (Benth). J. Sci. Indus. Res., 4: 187-195.
35. Chandra, V., 1957a. Leaf blotch disease of Rauvolfia serpentina. Sci. Cult., 23: 99-99.
36. Chandra, V., 1957b. Chromosome number of Rauwolfia serpentina. Curr. Sci., 3: 99-99.
37. Chatterjee, A. and S. Bose, 1951. A new alkaloid from the root of Rauwolfia serpentina benth. Sci. Cult, 17: 139-139.
38. De Britto, J. and R. Mahesh, 2007. Exploration of kani tribal botanical knowledge in agasthiayamalai biosphere reserve-south India. Ethnobotanical Leaflets, 11: 258-265.
39. De, J.N., 1965. Some minor plant-fibres of aboriginal usage in the district of Purulia (West Bengal). Bull. Bot. Soc. Bengal, 19: 67-72.
40. De, J.N., 1967. Further observation on the ethnobotany of Purulia district, West Bengal. Indian For., 95: 551-559.
41. De, J.N., 1968. Ethnobotany a newer science in India. Sci. Cult., 34: 326-328.
42. De, J.N., 1979a. Ethnobotanical study of Purulia District, West Bengal, The Eastern anthropologist. Lucknow, 32: 213-218.
43. De, M., 1979b. Chromosome studies on the genus Rauvolfia Linn. (Apocynaceae). Proc. Ind. Sci. Congr. Assoc, 66: 78-79.
44. De, J.N., 1980. The vegetarian- based tribal economics in the Purulia District West Bengal. Bull. Cult. Res. Inst, 14: 37-42.
45. Dhar, R., 1965. Variation in alkaloid content and morphology of four geographical races of Rauvolfia serpentina benth. Proc. Plant Sci., 62: 242-244.
46. Dnyansagar, V.R. and S.G. Torne, 1967. Mitosis and karyotype analysis of Rauvolfia serpentina. J. Biol. Sci., 10: 7-13.
47. Dnyansagar, V.R. and S.G. Torne, 1968. Meiotic analysis in Rauvolfia serpentina (L.) Benth. ex Kurz. Proc. Natn. Inst. Sci. India, 35: 368-376.
48. Dutta, P.K., S.B. Choudhury and P.R. Rao, 1962. Germination and chemical composition of Rauwolfia serpentina seeds. Ind. J. Pharm., 24: 61-63.
49. Dutta, P.K., I.C. Chopra and L.D. Kapoor, 1963. Cultivation of Rauvolfia serpentina in India. Econ. Bot, 17: 243-251.
50. Endreb, S., H. Takayama, S. Suda, M. Kitajima, N. Aimi, S. Sakai and J. Stockigt, 1993. Alkaloids from Rauwolfia serpentina cell cultures treated with ajmaline. Phytochemistry, 32: 725-730.
51. Fabricant, D.S. and N.R. Farnsworth, 2001. The value of plants used in traditional medicine for drug discovery. Environ. Health Perspect, 109: 69-75.
52. Falkenhagen, H. and J. Stockigt, 1993. Enzymatic biosynthesis of vomilenine, a keyintermediate of the ajmaline pathway, catalyzed by a novel cytochrome P 450 dependent enzyme from plant cell cultures of Rauvolfia serpentina. Z. Naturforsch. C: Biosci., 50: 45-53.
53. Falkenhagen, H., I.N. Kuzovkina, I.E. Alterman, L.A. Nikolaeva and J. Stockigt, 1993. Alkaloid formation in hairy roots and cell suspensions of Rauwolfia serpentina benth. Nat. Prod. Res., 3: 107-112.
54. Farooqi, A.A. and B.S. Sreeramu, 2001. Cultivation of Medicinal and Aromatic Crops. University Press, New Delhi, pp: 518.
55. Gerasimenko, I., Y. Sheludko and J. Stockigt 2001. 3-Oxo- rhazinilam: A new indole alkaloid from Rauvolfia serpentina X Rhazya stricta hybrid plant cell cultures. J. Nat. Prod., 64: 114-116.
56. Gerasimenko, I, Y. Sheludko, X. Ma and J. Stockigt 2002. Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem., 269: 2204-2213.
57. Ghani, A., 1998. Medicinal Plants of Bangladesh Chemical Constituents and Uses. Asiatic Society of Bangladesh, Dhaka.
58. Ghosh, K.C., G.N. Bhattacharya and N. Banerjee, 1998. A high regeneration potential of Rauwolfia serpentina Benth. through shoot tip culture: Chromosome analysis of the regenerants. Perspectives Cytol. Genet, 9: 313-318.
59. Glynn, J.D., 1955. Rauwolfia serpentina (Serpasil) in psychiatry. J. Neurol. Neurosurg. Psychiatry, 18: 225-227.
60. Goel, M.K., A.K. Kukreja and S.P.S. Khanuja, 2007. Cost effective approaches for in vitro mass propagation of Rauwolfia serpentina benth. ex Kurz. Asian J. Plant Sci.,6: 957-961.
61. Goel, M.K., S. Banerjee, K. Shanker and A.K. Kukreja, 2008. Exploring the potential of Agrobacterium rhizogenes mediated transformed roots of Rauwolfia serpentina for reserpine biosynthesis: One more step towards developing green factories. J. Biotechnol., 136: S168-S168.
62. Goel, M.K., S. Mehrotra, A.K. Kukreja, K. Shanker and S.P. Khanuja, 2009. In vitro propagation oiRauwolfia serpentina using liquid medium, assessment of genetic fidelity of micropropagated plants and simultaneous quantitation of reserpine, ajmaline and ajmalicine. Methods Mol. Biol., 547: 17-33.
63. Gorelova, O.A. and T.G. Korzhenevskaia, 2002. Formation of giant and ultramicroscopic forms of Nostoc muscorum CALU 304 during cocultivation with Rauwolfia tissues. Mikrobiologiia, 71: 654-661.
64. Gorelova, O.A. and S. Kleimenov, 2003. The accumulation and degradation dynamics of cyanophycin in cyanobacteria grown in symbiotic associations with plant tissues and cells. Mikrobiologiia, 72: 361-369.
65. Gupta, J.C., P.K. Roy, G.K. Ray and S.C. Ganguly, 1950. A preliminary note on the excretion of Rauwolfia total alkaloids in urine. Ind. J. Med. Res., 38: 67-73.
66. Habib, S.M. and W.E. Court, 1974. The separation and identification of microquantities of Rauwolfia alkaloids. Planta Med., 25: 331-341.
67. Hampp, N. and M.H. Zenk, 1988. Homogeneous strictosidine synthase from cell suspension cultures of Rauvolfia serpentina. Photochemistry, 27: 3811-3815.
68. Harisaranraj, H., K. Suresh and S. Saravanababu, 2009. Evaluation of the chemical composition Rauwolfia serpentina and Ephedra vulgaris. Adv. Biol. Res., 3: 174-178.
69. Hedayatullah, S., 1959. Culture and propagation of Rauwolfia serpentina benth. in East Pakistan. Pak. J. Sci. Indus. Res., 2: 118-122.
70. Hendrian, D.J.M., 1999. Revision of Rauvolfia (Apocynaceae) in Malesia. Blumea, 44: 449-470.
71. Hofmann, A., 1954. Rauhimbin und Isorauhimbin, zwei neue alkaloide aus Rauwolfia serpentina benth 2. Mitteilung uber rauwolfia-alkaloide. Helvet. Chim. Acta, 37: 314-320.
72. Holt W.L. and C.H. Costello, 1954. A preliminary chemical investigation oiRauwolfia serpentina. J. Am. Pharm. Assoc, 43: 144-148.
73. Holzbauer, M. and M. Vogt 1956. Depression by reserpine of the noradrenaline concentration in the hypothalamus of the cat. J. Neurochem., 1: 8-11.
74. Ilahi, I. and M. Akram, 1987a. Leaf callus culture of Rauwolfia serpentina. Pak. J. Bot, 19: 217-223.
75. Ilahi, I. and M. Akram, 1987b. In vitro propagation of Rauwolfia serpentina through lateral bud culture. Pak. J. Agnc. Res., 8: 204-210.
76. Ilahi, I., 1993. Micropropagation and biosynthesis of alkaloid by Rauvolfia serpentina cell culture. Proceedings of the International Plant Tissue Culture Conference, Dec. 19-21, Dhaka, pp: 21-21.
77. Ilahi, I. and M. Akram, 1993. Enhanced Propagation, Root Production and Alkaloid Biosynthesis by Cultures of Rauwolfia serpentina. In: Adapted Propagation Techniques for Commercial Crops of the Tropics, Quynh, N.T. and N. van Hyen (Eds.). Agricultural Publishing House, Ho Chi Mirth, Vietnam.
78. Ilahi, I., 1995. Rapid micro-propagation of Rauwolfia. Apam News Lett., 1: 35-36.
79. Ilahi, I., F. Rahim and M. Jabeen, 1995. Induction of somatic embryos and plant regeneration in Rauwolfia serpentina benth. Pak. J. Plant Sci., 1: 255-261.
80. Ilahi, I., F. Rahim and M. Jabeen, 2007. Enhanced clonal propagation and alkaloid biosynthesis in cultures of Rauwolfia. Pak. J. Plant Sci., 13: 45-56.
81. Itoh, A., T. Kumashiro, M. Yamaguchi, N. Nagakura, Y. Mizushina, T. Nishi and T. Tanahashi, 2005. Indole alkaloids and other constuents of Rauwolfia serpentina. J. Nat. Prod., 68: 848-852.
82. Jadhav, S.N., K.N. Reddy and C.S. Reddy, 2001. Conservation assessment and management planning for medicinal plants of Andhra Pradesh. Medicinal Plants Conservation Center (MPCC) and FRLHT, Hyderabad, India, pp: 1-39.
83. Jain, S.K. and J.N. De, 1964. Some less known plant foods among the tribals of Purulia (West Bengal). Sci. Cult, 30: 285-286.
84. Jain, S.K. and J.N. De, 1966. Observations of ethnobotany of Purulia, West Bengal. Bull. Bot. Survey India, 8: 237-251.
85. Janardhanan, K.K., D. Ganguli and A. Husain, 1964. Fusarium wilt of Rauvolfia serpentina. Curr. Sci., 30: 313-313.
86. Jigna, P., N. Rathish and C. Sumitra, 2005. Preliminary screening of some folklore medicinal plants from western India for potential antimicrobial activity. Indian J. Pharmacol, 37: 408-409.
87. Karim, M.A., W.H. Linnell and L.K. Sharp, 1961. Potential reserpine analogues Part II. 3,4,5-trimethoxybenzoic acid derivatives. J. Phar. Pharmacol., 12: 82-86.
88. Katana, V. and N.S. Shekhawat, 2005. Cloning of Rauvolfia serpentina-An endangered medicinal plant. J. Sustainable Forestry, 20: 53-65.
89. Kattel, L.P., 1987. Studies onphyllotaxical morphotypes in Rauvolfia serpentina (L.) benth. ex Kurz. J. Plant Anat. Morph., 4: 193-198.
90. Kaukhova, I.E., A.G. Vollosovich and V.A. Tsigankov, 1981. Nutrient medium selection for submerged culturing in Rauwolfia serpentina benth. tissues. Rast. Res., 17: 217-224.
91. Khatri, C.K., A. Qayum and S.M. Yusuf, 1982. Comparative studies on the effects of reserpine and its derivatives (bromo and dibromo) reserpine on the blood pressure, heart rate and EEG of rabbit. J. Pak. Med. Assoc, 32: 141-144.
92. Kirillova, N.V., M.G. Smirnova and V.P. Komov, 2001. Sequential isolation of superoxide dismutase and ajmaline from tissue culture oiRauwolfiia serpentina Benth. Prikl. Biokhim. Mikrobiol, 37: 181-185.
93. Kirillova, N.V. and V.P. Komov, 2002. Effect of phytohormones on the protein-synthesizing ability of Rauwolfia serpentina benth. tissue culture. Applied Biochem. Microbiol., 38: 45-47.
94. Kirillova, N. V., 2004. Changes in the activity of superoxide dismutase in Rauwolfia serpentina benth. Callus cultures grown under standard conditions and heat shock. Prikladnaya Biokhimiya i Mikrobiologiya, 40: 89-93.
95. Kirtikar, K.R. and B.D. Basu, 1993. Indian Medicinal Plants. 2 Edn., Dehra Dun Publishers. Culcutta, India, pp: 289.
96. Klushnichenko, V.E., S.Y. Yakimov, T.P. Bychkova, IN. Kuzovkina, AN. Vulf son and A.I. Miroshnikov, 1994. Separation of indole alkaloids from R. serpentina and R. vomitoria by HPLC and TLC methods. Pharma. Chem. J., 28: 855-859.
97. Klyushnichenko, V.E., S.A. Yakimov, T.P. Tuzova, Y.V. Syagailo, IN. Kuzovkina, AN. Wulfson and A.I. Miroshnikov, 1995. Determination of indole alkaloids from R. serpentina and R. vomitoria by high-performance liquid chromatography and high-performance thin-layer chromatography. J. Chromatogr. A, 704: 357-362.
98. Kostenyuk, LA., O.F. Lyubarets, S. Endre, Y.Y. Gleba and J. Stockigt, 1995. Alkaloids isolated from somatic hybrid cell cultures of the species combination Rauwolfia serpentina x rhazya stricta. Nat. Prod. Res., 5: 303-307.
99. Koul, M.L.H., 1964. Natural polyploidy in Rauwolfia serpentina. Sci. Res. Banaras Hindu Univ., 2: 100-102.
100. Kunakh, V.A. and E.G. Alkhimova, 1989. Rauwolfia serpentina: In vitro culture and the production of ajmaline. Biotechnol. Agric. Forestry, 7: 398-416.
101. Kunakh, V.A., 1996. Somaclonal Variation in Rauwolfia: Biotechnology in Agriculture and Forestry. In: Somaclonal Variation in Crop Improvement, Bajaj, Y.P.S. (Eds.). Vol. 36, Springer-Verlag, New York, pp: 315-332.
102. Kunakh, V.A., L.P. Mozhylevskaya and S.I. Gubar, 2001. Details of the method for obtaining and productivity of the suspension cultures and cell clones of Rauwolfia serpentina benth. in vitro. Biotechnology, 4: 9-21.
103. Kutchan, T.M., N. Hampp, F. Lottspeich, K. Beyreuther and M.H. Zenk, 1988. The cDNA clone for strictosidine synthase from Rauvolfia serpentine DNA sequence determination and expression in Escherichia coli. FEBS Lett, 27: 40-44.
104. Kutchan, T.M., 1989. Expression of enzymatically active cloned strictosidine synthase from the higher plant Rauvolfia serpentina in Escherichia coli. FEBS Lett, 257: 127-130.
105. Kutchan, T.M., A. Bock and H. Dittrich, 1994. Heterologous expression of the plant proteins strictosidine synthase and berberine bridge enzyme in insect cell culture. Phytochemistry, 35: 353-360.
106. Lele, C.V. and Ashram, 1968. Die-back disease of volfia serpentinased by letotrichum dematium. Ind. Phytopathol., 20: 349-350.
107. Lutterbach, R. and J. Stockigt, 1992. High-yield formation of arbutin from hydroquinone by cell suspension cultures of Rauwolfia serpentina. Helvetica Chimica Acta, 75: 2009-2011.
108. Ma, X., J. Koepke, A. Bayer, G. Fritzsch, H. Michel and J. Stockigt, 2005. Crystallization and preliminary X-ray analysis of native and selenomethionyl vinorine synthase from Rauvolfia serpentina. Acta Cryst, 61: 694-696.
109. Ma, X., S. Panjikar, J. Koepke, E. Loris and J. Stockigt, 2006. The structure of Rauvolfia serpentina strictosidine synthase is a novel six-bladed β-propeller fold in plant proteins. Plant Cell, 18: 907-920.
110. Madhusudan, K.P., S. Banerjee, S.P. Khanuja and S.K. Chattopadhyay, 2008. Analysis of Hairy root culture of Rauvolfia serpentina using direct analysis in real time mass spectrometric technique. Biomed. Chromatogr., 22: 596-600.
111. Maheshwari, S.K., B.N. Dahatonde, S. Yadav and S.K. Gangrade, 1985. Intercropping of Rauvolfia serpentina for higher monetary returns. Ind. J. Agric. Sci., 55: 332-334.
112. Maheshwari, S.K., O.P. Sharma, S.K. Gangrade and K.C. Trivedi, 1991. Irrigation schedule for sarpagandha (Rauvolfia serpentina) in a shallow black soil. Ind. J. Agric. Sci., 61: 169-171.
113. Mamgam, S.K., A.K. Goel and S.C. Sharma, 1998. Conservation of assessment of some important threatened medicinal plants of India. J. Non-Timber Fore. Prod., 5: 1-9.
114. Mao, A.A., T.M. Hynniewta and M. Sanjappa, 2009. Plant wealth of northeast India with reference to ethnobotany. Ind. J. Trad. Knowledge, 8: 96-103.
115. Mathur, A., A.K. Mathur, A.K. Kukreja, P.S. Ahuja and B.R. Tyagi, 1987. Establishment and multiplication of colchi autotetraploids of R. serpentina L. Benth ex Kurz. through tissue culture. Plant Cell Tissue Organ Cult, 10: 129-134.
116. Mathur, A., P.S. Ahuja and A.K. Mathur, 1993. Micro-Propagation of Panax quiquefoluim, Rauwolfia serpentina and Some other Medicinal and Aromatic Plants of India. In: Adapted Propagation Techniques for Commercial Crops of the Tropics, Quynh, N.T. and N.V. Hyen (Eds.). Agriculture Publishing House, Hochi Minh, Vietnam.
117. Mehrotra, M.D. and H.S. Thapar, 1990. Rhizoctonia leaf spotting and blight of Rauvolfia serpentina, a new disease from India. Ind. Forester, 16: 372-374.
118. Mia, M.M.K., M.F. Kadir, M.S. Hossan and M. Rahmatullah, 2009. Medicinal plants of the Garo tribe inhabiting the Madhupur forest region of Bangladesh. Am. Eurasian J. Sustainable Agric, 3: 165-171.
119. Mital, SP, M. Kazim, M.A. Kidwai and K.K. Mittal, 1980. Chemotypic Studies in natural populations of Rauvolfia serpentina from certail regions of Karnataka State, India. Curr. Sci., 49: 789-790.
120. Mitra, G.C. and K.N. Kaul, 1964. In vitro culture of root and stem callus of Rauwolfia serpentina benth. for reserpine. Ind. J. Exp. Biol., 2: 49-51.
121. Mitra, G.C, 1976. Studies on the formation of viable and nonviable seeds in R. serpentine. Ind. J. Exp. Biol., 14: 54-54.
122. Mohanta, R.K., S.D. Rout and H.K. Sahu, 2006. Ethnomedicinal plant resources of simlipal biosphere reserve, Orissa, India. Zoo's Print J., 21: 2372-2374.
123. Mohanty, N.N. and S.K. Addy, 1958. Target spot of Rauvolfia serpentina. Sci. Cult., 23: 608-609.
124. Monachino, J., 1954. Rauvolfia serpentina: Its history, botany and medical use. Econ Bot, 8: 349-365.
125. Mukhopadhyaya, S., M.J. Mukhopadhyaya and A.K. Shrma, 1991. In vitro multiplication and regeneration of cytologically stable platns of Rauwolfia serpentina Benth. through shoot tip culture. Nucleus Calcutta, 34: 170-173.
126. Muscholl, E. and M. Vogt, 1958. The action of reserpine on the peripheral sympathetic system. J. Physiol., 141: 132-155.
127. Nayar, S.L., 1956. Experimental propagation and culture of Rauwolfia serpentina benth. by seeds. Ind. J. Pharm., 18: 125-126.
128. Nayar, M.P. and A.R.K. Sastry, 1987. Red Data Book of Indian Plants. Vol. 1, Botanical Survey of India, Calcutta.
129. Nayar, M.P. and A.R.K. Sastry, 1988. Red Data Book of Indian Plants. Vol. 2, Botanical Survey of India, Calcutta.
130. Nayar, M.P. and A.R.K. Sastry, 1990. Red Data Book of Indian Plants. Vol. 3. Botanical Survey of India, Calcutta.
131. Nayar, M.P., 1996. Hot Spots of Endemic Plants in India, Nepal and Bhutan. Tropical Botanical Garden and Research Institute, Thiruvananthapuram, Kerala, pp: 252.
132. Noce, R.H., D.B. Williams and W. Rapaport, 1954. Reserpine (Serpasil) in the management of mentally ill and mentally retarded. J. Am. Med. Assoc, 156: 821-824.
133. Nurcahyani, N, Solichatun and E. Anggarwulan, 2008. The reserpine production and callus growth of indian snake root (Rauvolfia serpentina (L.) benth. Ex Kurz) culture by addition of Cu2+. Biodiversitas, 9: 177-179.
134. Obitz, P., S. Endreb and J. Stockigt 1995. Enzymatic biosynthesis of raumacline. Phytochemistry, 40: 1407-1417.
135. Padmalatha, K. and M.N.V. Prasad, 2007. Inter and intrapopulation genetic diversity of Rauvolfia serpentina (L.) benth. ex Kurz, an endangered medicinal plant, by RAPD analysis. Med. Aromatic Plant Sci. Biotechnol, 1: 118-123.
136. Pakrashi, S. and B. Akkhari, 1968. Rauwolfia alkaloids. Farmatsiia, 17: 64-82.
137. Pandey, B.P., 1984. Economic Botany. Janta Vedic College, Baraut, India.
138. Pandey, V.P., J. Kudakasseril, E. Cherian and G. Patani, 2007. Comparison of two methods for in vitro propagation of Rauwolfia serpentina from nodal explants. Ind. Drugs, 44: 514-519.
139. Pandey, V.P., E. Cherian and G. Patani, 2010. Effect of growth regulators and culture conditions on direct root induction of Rauwolfia serpentina L. (Apocynaceae) benth by leaf explants. Trop. J. Pharm. Res., 9: 27-34.
140. Pant, K.K. and S.D. Joshi, 2008. Rapid multiplication of Rauvolfia serpentina benth. ex. Kurz through tissue culture. Sci. World, 6: 58-62.
141. Parmitha, M., G.U. Harish, N.C. Vivek, T. Mahesh and M.B. Shivanna, 2004. Ethnobotanical wealth of Bhadra wild life Sanctuary, Karnataka. India J. Traditional Knowledge, 3: 37-50.
142. Patil, V.M. and M. Jayanthi, 1997. Micropropagation of two species of Rauwolfia (Apocyanaceae). Curr. Sci., 72: 961-965.
143. Pattanaik, C, S. Reddy and K.N. Reddy, 2009. Ethno-medicinal survey of threatened plants in eastern ghats, India. Our Nature, 7: 122-128.
144. Paul, D., N.K. Paul and P.K. Basu, 2008. Seed germination response of Rauvolfia serpentna benth. to certain physical and chemical treatments. J. Bio-Sci., 16: 129-131.
145. Perveen, R. and I. Ilahi, 1978. Callus formation in Rauwolfia serpentina. Pak. J. Bot, 10: 141-148.
146. Pletscher, A., P.A. Shore and B.B. Brodie, 1955. Serotonin release as a possible mechanism of reserpine action. Science, 122: 374-375.
147. Polz, L., J. Stockigt H. Takayama, N. Ushida, N. Aimi and S.I. Sakai, 1990. Biotransformation of ajmaline inplant cell cultures of Rauwolfia serpentina benth.: The new indole alkaloids raumacline and N(β)-methyl-raumacline. Tetrahedron Lett., 31: 6693-6696.
148. Popelak, A., M. Spingler, F. Kaiser, J.D. Achelis and G. Kroneberg, 1953. Neue alkaloide aus Rauwolfia serpentina. Naturwissenchaften, 40: 625-625.
149. Popov, S.V., V.G. Vinter, O.A. Patova, P.A. Markov and I.R. Nikitina et al., 2007. Chemical characterization and anti-inflammatory effect of rauvolfin, a pectic polysaccharide of Rauvolfia callus. Biochemistry, 72: 778-784.
150. Raghavan, R.S., 1957. Chromosome numbers in Indian medicinal plants. Proc. Ind. Acad. Sci., 6: 294-298.
151. Rai, S.K., 2004. Medicinal plants used by meche people of Jhapa district, eastern Nepal. Our Nature, 2: 27-32.
152. Raj, A.D.S. and S. Sukumaran, 2008. Rare and endemic plants in the sacred groves of akumari district in Tamil Nadu. Ind. J. Forestry, 31: 611-616.
153. Rajkarnikar, K.M., H.K. Sainju and G.D. Bhatta, 2000. In vitro culture oiRauwolfia serpentina L. benth. ex. Kurz. Proceedings of Nepal Japan Joint Symposium, pp: 232-234.
154. Ray, A. and S. Bhattacharya, 2008. Cryopreservation of in vitro grown nodal segments of Rauvolfia serpentina by PVS2 vitrification. Cryoletters, 29: 321-328.
155. Roja, P.C., B.D. Benjamin, M.R. Heble and M.S. Chadha, 1985. Indole alkaloids from multiple shoot cultures of R. serpentina. Plant. Med., 50: 73-74.
156. Roja, P.C., A.T. Sipahimalini, M.R. Heble and M.S. Chadha, 1987. Multiple shoot cultures of R. serpentina: Growth and alkaloid production. J. Nat. Prod., 50: 872-875.
157. Roja, G. and M.R. Heble, 1996. Indole alkaloids in clonal propagules of Rauwolfia serpentina Benth. ex. Kurz. Plant Cell Tissue Organ Culture, 44: 111-115.
158. Rout S.D., T. Panda and N. Mishra, 2009. Ethno-medicinal plants used to cure different diseases by tribals of mayurbhanj district ofNorth orissa. Ethno-Med., 3: 27-32.
159. Rout S.D., S.K. Panda, N. Mishra and T. Panda, 2010. Role of tribals in collection of commercial non-timber forest products in Mayurbhanj district, Orissa. Stud. Tribes Tribals, 8: 21-25.
160. Roy, S.K., M.Z. Hossain and M.S. Islam, 1994. Mass propagation of Rauwolfia serpentina by in vitro shoot tip culture. Plant Tissue Cult, 4: 69-75.
161. Ruppert, M., J. Woll, A. Giritch, E. Genady, X. Ma and J. Stockigt, 2005a. Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system. Planta, 222: 888-898.
162. Ruppert M., X. Ma and J. Stockigt 2005b. Alkaloid biosynthesis in Rauvolfia- cDNA cloning of major enzymes of the ajmaline pathway. Curr. Organic Chem.,9: 1431-1444.
163. Ruyter, CM., A. Mahmood, I. Illahi and J. Stockigt, 1991. Investigation of the alkaloid content of Rauwolfia serpentina roots from regenerated plants. Planta Med., 57: 328-330.
164. Saini, S., A. Sattar and M. Alam, 1996. Occurrence of stem rot disease caused by Rhizopus stolonifer on periwinkle. Ind. J. Plant Pathol, 14: 1-4.
165. Salma, U., M.S.M. Rahman, S. Islam, N. Haque, T.A. Jubair, A.K.M.F. Haque and I.J. Mukti, 2008a. The influence of different hormone concentration and combination on callus induction and regeneration of Rauwolfia serpentina L. Benth. Pak. J. Biol. Sci., 11: 1638-1641.
166. Salma, U, M.S.M. Rahman, S. Islam, N. Haque, M. Khatun, T.A. Jubair and B.C. Paul, 2008b. Mass propagation of Rauwolfia serpentina L. Benth. Pak. J. Biol. Sci., 11: 1273-1277.
167. Sankaranarayanan, S., P. Bama, J. Ramachandran, P.T. Kalaichelvan and M. Deccaraman et al., 2010. Ethnobotanical study of medicinal plants used by traditional users in Villupuram district of Tamil Nadu. India J. Med. Plants Res., 4: 1089-1101.
168. Santapau, H., 1956. Botanical aspects of R. serpentina. Ind. J. Pharm., 18: 117-126.
169. Sarkar, K.P., A. Islam, R. Islam, A. Hoque and O.I. Jorarder, 1996a. In vitro propagation of R. serpentina through tissue culture. Plant Med., 62: 358-359.
170. Sarkar, K.P., A. Islam, R. Islam, A. Hoque and O.I. Joardar, 1996b. Plant regeneration of Rauvolfia serpentina by organogenesis from callus cultures. Plant Tissue Cult., 6: 63-65.
171. Sarkar, N, S. Rudra and S.K. Basu, 1999. Ethnobotany of Bangiriposi, Mayurbhanj, Orissa. J. Econ. Tax. Bot, 23: 509-514.
172. Sarma, D., A.K. Kukreja and A. Baruah, 1997. Transforming ability of two Agrobacterium rhizogenes strains in Rauvolfia serpentina (L.) leaves. Ind. J. Plant Physiol., 2: 166-168.
173. Saxena, H.O., M. Brahmam and P.K. Dutta, 1988. Ethnobotanical studies in similipal forests of Mayurbhanj district (Orissa). Bull. Bot. Surv. India, 10: 83-89.
174. Schlemmer, F. and F. Link, 1959. Thin-layer chromatography of rauwolfia alkaloids. Pharm. Ztg., 104: 646-648.
175. Schlittler, E., H. Saner and J.M. Muller, 1954. Reserpinin, ei neues alkaloid aus Rauwolfia serpentina. Expenentia, 10: 109-133.
176. Sehrawat, A.R., U. Sanjogta and A. Punia, 2001. In vitro culture and multiplication of Rauwolfia serpentina-A threatened medicinal plant. Crop Res., 22: 68-71.
177. Sen, S. and P.C. Datta, 1990. Use of hormones in vitro for alkaloid production in some Apocynaceous drugs. Acta Bot. Sin., 18: 115-117.
178. Sen, P., M. Dollo, M. Duttachoudhury and D. Choudhury, 2008. Documentation of traditional herbal knowledge of Khamptis of arunachal Pradesh. Ind. J. Tradit. Knowledge, 7: 438-442.
179. Sethi, K.L. and M. Kazim, 1983. Collection and conservation of Rauvolfia serpentina genetic resources of South Karnataka and Western Ghats of India. Proceedings of the 5th All India Workshop on Medicinal and Aaromatic Plants, Oct. 83, Solan, Indian, pp: 1-8.
180. Sethi, K.L., M. Kazim, M.A. Kidwai and S.P. Mital, 1991. Variation of chemo-botanical characters in the indigenous collections of Rauvolfia serpentina. Ind. J. Genet. Plant Breed., 51: 134-138.
181. Shamsi, Y., H. Kumar, S.A. Tamanna and E.A. Khan, 2006. Effect of a polyherbal Unani formulation on chronic urticaria. Ind. J. Tradit. Knowledge, 5: 279-283.
182. Sharma, S., 1958. Medicinal plants of India. Illustrated Weekly of India, January, pp: 43-44.
183. Sharma, A.K. and M. De, 1976. Cytotaxonomic studies on medicinal plants of Apocynaceae. Res. Bull. Univ. Calcutta Cytogenetics Lab., 3: 41-42.
184. Sharma, N. and K.P.S. Chandel, 1992. Low temperature storage of Rauvolfia serpentina benth. ex Kurz.: An endangered, endemic medicinal plant. Plant Cell Rep., 11: 200-203.
185. Sheludko, Y.V. and J.A. Kostenyuk, 1994. Growth dynamics and total alkaloid content in transgenic root culture of Rauvolfia serpentina benth. Tsitologiya i Genetika, 28: 35-38.
186. Sheludko, Y., I. Gerasimenko and O. Platonova, 2000. Divergence of the indole alkaloid pattern in two somatic hybrid plant cell subcultures of Rauvolfia serpentina X rhazya stricta. Planta Med., 66: 656-659.
187. Sheludko, Y., I. Gerasimenko, H. Kolshorn and J. Stockigt, 2002a. Isolation and structure elucidation of a new indole alkaloid from Rauvolfia serpentina hairy root culture: The first naturally occurring alkaloid of the raumacline group. Planta Med., 68: 435-439.
188. Sheludko, Y., I. Gerasimenko, H. Kolshorn and J. Stockigt, 2002b. New alkaloids of the sarpagine group from Rauvolfia serpentina, hairy root culture. J. Nat. Prod., 65: 1006-1010.
189. Shen, Z., W. Eisenreich and T.M. Kutchan, 1998. Bacterial biotransformation of 3α(S) strictosidine to the monoterpenoid indole alkaloid vallesiachotamine. Phytochemistry, 48: 293-296.
190. Sheppard, H. and J.H. Zimmerman, 1960. Reserpine and the levels of serotonin and norepinephrine in the brain. Nature, 185: 40-41.
191. Shimolina, L.L., T.V. Astakhova and L.A. Nikolafva, 1984. Quantitative determination of total alkaloids in rauwolfia serpentina tissue culture. Rastit. Resur., 20: 137-141.
192. Shukla, R.S., M. Alain, A. Sattar, Abdul-Khaliq and H.N. Singh, 2006. First report of Rhizopus stolonifer causing inflorescence and fruit rot of Rauvolfia serpentina in India. EPPO Bull., 36: 11-13.
193. Siddiqui, S.S. and R.H. Siddiqui, 1931. The alkaloids of Rauwolfia serpentina, benth. J. Ind. Chem. Soc, 8: 667-678.
194. Siddiqui, S.S. and R.H Siddiqui, 1932. The alkaloids of Rauwolfia serpentina, benth. J. Ind. Chem. Soc, 9: 539-542.
195. Siddiqui, S.S. and R.H Siddiqui, 1935. The alkaloids of Rauwolfia serpentina, benth. Part II. Studies in ajmaline series. J. Ind. Chem. Soc, 12: 37-37.
196. Siddiqui, S.S., 1939. A note on the alkaloids Rauwolfia serpentina, benth. J. Ind. Chem. Soc, 16: 421-425.
197. Singh, D.N., 1961. Use of induced rooting in cytological studies. Nature, 189: 420-421.
198. Singh, D.K., B. Srivastava and A. Sahu, 2004. Spectophotometric determination of Rauwolfia alkaloids: Estmation of reserpine in pharmaceuticals. Anal. Sci.,20: 571-573.
199. Singh, H., 2008. Importance of local names of some useful plants in ethnobotanical study. Ind. J. Tradit. Knowledge, 7: 365-370.
200. Singh, P.K., V. Kumar, R.K. Tiwari, A. Sharma, C. V. Rao and R.H. Sinhg, 2010. Medico-ethnobotany of chatara block of district sonebhadra, Uttar Pradesh, India. Adv. Biol. Res., 4: 65-80.
201. Solovyan, V.T., E.V. Spindonova and V.A. Kunakh, 1994a. Genome rearrangements in cultured Rauwolfia serpentina cells. I. The involvement of the multiple genomic sequences. Genetika, 30: 250-254.
202. Solovyan, V.T., E. V. Spiridonova and V.A. Kunakh, 1994b. Genome rearrangements in cultured Rauwolfia serpentina cells. II. Relation to the interspecies variation. Genetika, 30: 399-401.
203. Solovyan, V.T., V.A. Kunakh, A.V. Vershinin and V.K. Shumny, 1986. The comparison of the DNA homology degree and the number of repeated sequences in intact plant and cultured cells of Rauwolfia serpentina benth. Proc Nat. Acad. Sci., 287: 998-1001.
204. Spiridonova, E.V., D.M. Adnof, I.O. Andreev and V.A. Kunakh, 2008. Dynamics of genome changes in Rauwolfia serpentina callus tissue upon the switch to conditions of submerged cultivation. Cytol. Genet., 42: 101-106.
205. Sreemantula, S., K.M. Boini and S. Nammi, 2004. Reserpine methonitrate, a novel quaternary analogue of reserpine augments urinary excretion of VMA and 5-HIAA without affecting HVA in rats. BMC Pharmacol., 4: 30-30.
206. Srivastava, A., A.K. Tripathi, R. Pandey, R.K. Verma and M.M. Gupta, 2006. Quantitative determination of reserpine, ajmaline and ajmalicine in Rawvolfia serpentine by reversed-phase high-performance liquid chromatography. J. Chromatogr. Sci., 44: 557-560.
207. Stanford, J.L., E. J. Martin, L.A. Brintin and R.N. Hoover, 1986. Rauwolfia use and breast cancer: A case-control study. J. Natl. Cancer Inst, 76: 817-822.
208. Stockigt, J., A. Pfitzner and J. Firl, 1981. Indole alkaloids from cell suspension cultures of Rauwolfia serpentina benth. Plant Cell Rep., 1: 36-39.
209. Stockigt, J., 1995. Biosynthesis in Rauwolfia serpentina Modern Aspects of An Old Medicinal Plant. In: The Alkaloids, Chemistry and Pharmacology, Cordell, G.A. (Eds.). Academic Press, New York, pp: 115-172.
210. Sultana, S.S., A.N.M. Rubaiyath-Bin-Rahman and S.S. Alain, 2009. Karyotype analysis with orcein and CMA from leaf base cells of Rauvolfia serpentina benth. exKurz. Bangladesh J. Bot, 38: 111-113.
211. Tapadar, N, N. Roy and N.K. Sen, 1960. Cytotaxonomical studies on the economic plants of the family Apocynaceae. Caryologia, 3: 367-397.
212. Tapadar, N. and N. Roy, 1964. Cytotaxonomic studies in apocynaceae and delineation of the different evolutionary tendencies operating within the family. Caryologia, 77: 103-138.
213. Trcka, V. and A. Carlsson, 1967. Pharmcology of reserpine analogues VI. Reserpine analogs with differential effect on brain monoamines. Acta Pharmacol. Toxicol, 25: 107-112.
214. Uesato, S., S. Kanomi, A. Iida, H. Inouye and M.H. Zenka, 1986. Mechanism for iridane skeleton formation in the biosynthesis of secologanin and indole alkaloids in Lonicera tatarica, Catharanthus roseus and suspension cultures of Rauwolfia serpentina. Phytochemistry, 25: 839-842.
215. Vaidya, A.D.B., 2006. Reverse pharmacological correlates of Ayurvedic drug actions. Ind. J. Pharmacol., 38: 311-315.
216. Vakil, R.J., 1955. Rauwolfia serpentina in the treatment of high blood pressure. Circulation, 12: 220-229.
217. Van der Laan, F.M. and J.C. Arends, 1985. Cytotaxonomy of the apocynaceae. Genetica, 68: 3-35.
218. Varadarajan, P.D., 1958. Blight and die-back disease of Rauvolfia serpentina. J. Biol., 1: 1-4.
219. Varadarajan, P.D., 1964. Anthracnose disease of Rauvolfia serpentina caused by Colletotrichum gloeosporioides. Curr. Sci., 33: 564-565.
220. Varadarajan, P.D., 1967. Leaf spot and premature defoliation of Rauvolfia serpentina under cultivation caused by Curvularia lunata. Ind. Phytopathol., 19: 298-300.
221. Varchi, G., A. Battaglia, C. Samori, E. Baldelli and B. Danieli et al., 2005. Synthesis of deserpidine from reserpine. J.Nat. Prod, 68: 1629-1631.
222. Vollosovich, A.G. and R.G. Butenka, 1970. Tissue Culture of Rauwolfia serpentina as a Resource of Alkaloids. In: Culture of Isolated Organs, Tissues and Cells of Plant, Butenka, R.G. (Eds.). Nauka, Moscow, pp: 253-257.
223. Vollosovich, N.E., A.G. Vollosovich, T.A. Kovaleva, Z.B. Shamina and R.G. Butenko, 1976. Strains of a Rauwolfia serpentina benth. Tissue culture and their productivity. Rast. Res., 12: 578-583.
224. Vollosovich, A.G., L.A. Nikolaeva, S.G. Duksina and L.K. Kartashova, 1977. Methods for the quantitative determination of the sum of alkaloids in a tissue culture of rauwolfia (Rauwolfia serpentina benth). Farmatsiia, 26: 29-32.
225. Vollosovich, A.G., T.M. Puchinina and L.A. Nikolaeva, 1979. Optimization of the composition of macrosalts for Rauwolfia serpentina benth. tissue culture. Rastit. Res., 15: 516-526.
226. Vollosovich, A.G., T.M. Puchinina and N.A. Listunova, 1982. Optimization of macronutrient composition for Rauwolfia tissue culture. Rast. Res., 15: 239-243.
227. Von Poser, G., H.H. Andrade, K.V. Da Silva, A.T. Henriques and J.A. Henriques, 1990. Genotoxic, mutagenic and recominogenic effects of Rauwolfia alkaloids. Mutat. Res., 232: 37-43.
228. Wachsmuth, O. and R. Matusch, 2002. Anhydronium bases from Rauvolfia serpentina. Phytochemistry, 61: 705-709.
229. Wakhloo, J.L., 1963. Variation in the total alkaloid content of Rauvolfia serpentina roots, a consideration from ecological point of view. J. Ind. Bot. Soc, 42: 215-215.
230. Warzecha, H., P. Obitz and J. Stockigt, 1999. Purifcation, partial amino acid sequence and structure of the product of raucaffricine-0-β-D-glucosidase from plant cell cultures of Rauwolfia serpentina. Phytochemistry, 50: 1099-1109.
231. Yamamoto, O. and Y. Yamada, 1986. Production of reserpine and its optimization in cultured Rauwolfia serpentina Benth. cells. Plant Cell Rep., 5: 50-53.
232. Yamamoto, O. and Y. Yamada, 1987. Selection of a reserpine-producing cell strain using UV-light and optimization of reserpine production in the selected cell strain. Plant Cell Tissue Organ Cult., 8: 125-133.