Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids

European Journal of Biochemistry

Volumn 269, Issue 8, 2002, Pages 2204-2213

  Gerasimenko, Irina ^a   Sheludko, Yuri ^a   Ma, Xueyan ^a   Stöckigt, Joachim ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Ajmaline; Heterologous expression; Indole alkaloid biosynthesis; Rauvolfia serpentina; Strictosidine D glucosidase

Indexed keywords

3 ISOCORREANTINE A; 4,21 DEHYDROCORYNANTHEINE ALDEHYDE; ALDEHYDE DERIVATIVE; AMINE; CATHENAMINE; CHEMICAL COMPOUND; COMPLEMENTARY DNA; GLUCOSIDASE; GLYCOSIDASE; INDOLE ALKALOID; PROTEIN; STRICTOSIDINE; TERPENOID; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BIOSYNTHESIS; CATHARANTHUS ROSEUS; CELL CULTURE; CELL SUSPENSION; CONTROLLED STUDY; DNA SEQUENCE; ENDOPLASMIC RETICULUM; ENZYMATIC ASSAY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC CODE; GLYCOSYLATION; HETEROLOGOUS EXPRESSION; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; PH; PLANT; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN TRANSPORT; RAUVOLFIA SERPENTINA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TEMPERATURE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GLUCOSIDASES; INDOLE ALKALOIDS; MOLECULAR SEQUENCE DATA; RAUWOLFIA; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; VINCA ALKALOIDS;

CATHARANTHUS ROSEUS; ESCHERICHIA COLI; RAUVOLFIA; RAUVOLFIA SERPENTINA;

EID: 0036233184     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.02878.x     Document Type: Article

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