Rapid bacterial artificial chromosome modification for large-scale mouse transgenesis

Nature Protocols

Volumn 5, Issue 10, 2010, Pages 1678-1696

  Gong, Shiaoching ^a   Kus, Laura ^a   Heintz, Nathaniel ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; RECA PROTEIN; RECOMBINASE;

ACCURACY; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; CHEMICAL MODIFICATION; CHROMOSOME REPLICATION; CONTROLLED STUDY; GENE CASSETTE; GENETIC RECOMBINATION; HIGH THROUGHPUT SCREENING; LARGE SCALE PRODUCTION; MARKER GENE; MOUSE; NIDATION; NONHUMAN; PLASMID; PRIORITY JOURNAL; REPORTER GENE; TEMPERATURE SENSITIVITY; TRANSGENIC MOUSE; TRANSGENICS;

ANIMALS; CHROMOSOMES, ARTIFICIAL, BACTERIAL; DATABASES, GENETIC; DNA, BACTERIAL; ESCHERICHIA COLI; FLUORESCENT DYES; GENE LIBRARY; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; MICE; MICE, TRANSGENIC; REC A RECOMBINASES; RECOMBINATION, GENETIC; REPLICATION ORIGIN; TRANSGENES;

BACTERIA (MICROORGANISMS); MUS; MUS MUSCULUS;

EID: 77957558274     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.131     Document Type: Article

References (34)

1. Monaco, A.P. & Larin, Z. YACs, BACs, PACs and MACs: artifcial chromosomes as research tools. Trends Biotechnol. 12, 280-286 (1994).
2. Giraldo, P. & Montoliu, L. Size matters: use of YACs, BACs and PACs in transgenic animals. Transgenic. Res. 10, 83-103 (2001).
3. Shizuya, H. et al. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89, 8794-8797 (1992).
4. Ioannou, P.A. et al. A new bacteriophage P1-derived vector for the propagation of large human DNA fragments. Nat Genet. 6, 84-89 (1994).
5. Marra, M.A. et al. High throughput fngerprint analysis of large-insert clones. Genome Res. 7, 1072-1084 (1997).
6. Kelley, J.M. et al. High throughput direct end sequencing of BAC clones. Nucleic Acids Res. 27, 1539-1546 (1999).
7. Osoegawa, K. & de Jong, P.J. BAC library construction. Methods Mol. Biol. 255, 1-46 (2004).
8. Mozo, T. et al. A complete BAC-based physical map of the Arabidopsis thaliana genome. Nat Genet. 22, 271-275 (1999).
9. Hoskins, R.A. et al. A BAC-based physical map of the major autosomes of Drosophila melanogaster. Science 287, 2271-2274 (2000).
10. Erratum in Science 288, 1751 (2000).
11. Osoegawa, K. et al. Bacterial artifcial chromosome libraries for mouse sequencing and functional analysis. Genome Res. 10, 116-128 (2000).
12. McPherson, J.D. et al. A physical map of the human genome. Nature 409, 934-941 (2001).
13. Shizuya, H. & Kouros-Mehr, H. The development and applications of the bacterial artifcial chromosome cloning system. Keio J. Med. 50, 26-30 (2001).
14. Yang, X.W., Model, P. & Heintz, N. Homologous recombination based modifcation in Escherichia coli and germline transmission in transgenic mice of a bacterial artifcial chromosome. Nat Biotechnol. 15, 859-865 (1997).
15. Heintz, N. BAC to the future: the use of bac transgenic mice for neuroscience research. Nat Rev. Neurosci. 2, 861-870 (2001).
16. Lalioti, M. & Heath, J.A. A new method for generating point mutations in bacterial artifcial chromosomes by homologous recombination in Escherichia coli. Nucleic Acids Res. 29, E14 (2001).
17. Gong, S., Yang, X.W., Li, C. & Heintz, N. Highly effcient modifcation of bacterial artifcial chromosomes (BACs) using novel shuttle vectors containing the R6Kgamma origin of replication. Genome Res. 12, 1992-1998 (2002).
18. Ohtsuka, M., Kimura, M., Tanaka, M. & Inoko, H. Recombinant DNA technologies for construction of precisely designed transgene constructs. Curr. Pharm. Biotechnol. 10, 244-251 2009.
19. Zhang, Y., Buchholz, F., Muyrers, J.P. & Stewart, A.F. A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet. 20, 123-128 (1998).
20. Murphy, K.C. Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli. J. Bacteriol. 180, 2063-2071 (1998).
21. Sharan, S.K., Thomason, L.C., Kuznetsov, S.G. & Court, D.L. Recombineering: a homologous recombination-based method of genetic engineering. Nat Protoc. 4, 206-223 (2009).
22. Lee, E.C. et al. A highly effcient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA. Genomics 73, 56-65 (2001).
23. Metcalf, W.W. et al. Conditionally replicative and conjugative plasmids carrying lacZ alpha cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35, 1-13 (1996).
24. Gay, P., Le Coq, D., Steinmetz, M., Berkelman, T. & Kado, C.I. Positive selection procedure for the entrapment of insertion sequence elements in gram-negative bacteria. J. Bacteriol. 164, 918-921 (1985).
25. Gong, S. et al. A gene expression atlas of the central nervous system based on bacterial artifcial chromosomes. Nature 425, 917-925 (2003).
26. Wiedenmann, J., Oswald, F. & Nienhaus, G.U. Fluorescent proteins for live cell imaging: opportunities, limitations, and challenges. IUBMB Life 61, 1029-1042 (2009).
27. Heiman, M. et al. A translational profling approach for the molecular characterization of CNS cell types. Cell 135, 738-748 (2008).
28. Doyle, J.P. et al. Application of a translational profling approach for the comparative analysis of CNS cell types. Cell 135, 749-762 (2008).
29. Chrast, R., Scott, H.S. & Antonarakis, S.E. Linearization and purifcation of BAC DNA for the development of transgenic mice. Transgenic Res. 8, 147-150 (1999).
30. Gong, S. & Yang, W.X. Modifcation of bacterial artifcial chromosomes (BACs) and preparation of intact BAC DNA for generation of transgenic mice. Curr. Protoc. Neurosci. Chapter 5: Unit 5.21 (2005).
31. Conner, D.A. Transgenic mouse production by zygote injection. Curr. Protoc. Mol. Biol. Chapter 23: Unit 23.9 (2004).
32. Geschwind, D. GENSAT: a genomic resource for neuroscience research. Lancet Neurol. 3, 82 (2004).
33. Hatten, M.E. & Heintz, N. Large-scale genomic approaches to brain development and circuitry. Annu. Rev. Neurosci. 28, 89-108 (2005).
34. Abe, K., Hazama, M., Katoh, H., Yamamura, K. & Suzuki, M. Establishment of an effcient BAC transgenesis protocol and its application to functional characterization of the mouse Brachyury locus. Exp. Anim. 53, 311-320 (2004).