Efficient production and purification of functional bacteriorhodopsin with a wheat-germ cell-free system and a combination of Fos-choline and CHAPS detergents

Biochemical and Biophysical Research Communications

Volumn 400, Issue 4, 2010, Pages 638-642

  Genji, Takahisa ^a   Nozawa, Akira ^a,b   Tozawa, Yuzuru ^a  

^a EHIME UNIVERSITY   (Japan)

^b RIKEN   (Japan)

Author keywords

Bacteriorhodopsin; Cell free expression; Membrane protein; Micelle

Indexed keywords

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; BACTERIORHODOPSIN; PROTEIN FOS;

ARTICLE; BATCH PROCESS; CELL FREE SYSTEM; GRAIN YIELD; INHIBITION KINETICS; MICELLE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; WHEAT GERM;

BACTERIORHODOPSINS; CELL-FREE SYSTEM; CHOLIC ACIDS; DETERGENTS; GERM CELLS; MICELLES; PHOSPHORYLCHOLINE; PROTEIN BIOSYNTHESIS; TRITICUM;

TRITICUM AESTIVUM;

EID: 77957258385     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.08.119     Document Type: Article

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