Live cell imaging of micronucleus formation and development

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 692, Issue 1-2, 2010, Pages 12-18

  Yasui, Manabu ^a   Koyama, Naoki ^b   Koizumi, Tomoko ^a   Senda Murata, Kaori ^c   Takashima, Yoshio ^d   Hayashi, Makoto ^e   Sugimoto, Kenji ^c   Honma, Masamitsu ^a  

^a NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

^b UNIVERSITY OF SHIZUOKA   (Japan)

^c Osaka Prefecture University   (Japan)

^d NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES   (Japan)

^e Biosafety Research Center   (Japan)

Author keywords

Aneuploid; Chromosome aberration; M phase; Micronuclei; Micronucleus test

Indexed keywords

ALPHA TUBULIN; ANEUGEN; CLASTOGEN; HISTONE H3; MITOMYCIN C; VINCRISTINE;

ANAPHASE; ARTICLE; BIOLOGICAL MONITORING; CELL CYCLE PROGRESSION; CELL DEATH; CELL DIVISION; CHROMOSOME SEGREGATION; CONTROLLED STUDY; CYTOCHEMISTRY; DAUGHTER CELL; ENVIRONMENTAL EXPOSURE; GAMMA RADIATION; GENETIC STABILITY; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; LYMPHOBLASTOID CELL LINE; METAPHASE; MICRONUCLEUS; MICRONUCLEUS TEST; NONHUMAN; PRIORITY JOURNAL; SUPERNUMERARY CHROMOSOME;

CELL DIVISION; CELL LINE; FLUORESCENCE; GAMMA RAYS; HUMANS; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; MUTAGENS;

EID: 77957112764     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrfmmm.2010.07.009     Document Type: Article

References (33)

1. Weng H., Morimoto K. Differential responses to mutagens among human lymphocyte subpopulations. Mutat. Res. 2009, 672:1-9.
2. Holland N., Bolognesi C., Kirsch-Volders M., Bonassi S., Zeiger E., Knasmueller S., Fenech M. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps. Mutat. Res. 2008, 659:93-108.
3. Mateuca R., Lombaert N., Aka P.V., Decordier I., Kirsch-Volders M. Chromosomal changes: induction, detection methods and applicability in human biomonitoring. Biochimie 2006, 88:1515-1531.
4. Fenech M. The in vitro micronucleus technique. Mutat. Res. 2000, 455:81-95.
5. El Zein R.A., Schabath M.B., Etzel C.J., Lopez M.S., Franklin J.D., Spitz M.R. Cytokinesis-blocked micronucleus assay as a novel biomarker for lung cancer risk. Cancer Res. 2006, 66:6449-6456.
6. Bonassi S., Znaor A., Ceppi M., Lando C., Chang W.P., Holland N., Kirsch-Volders M., Zeiger E., Ban S., Barale R., Bigatti M.P., Bolognesi C., Cebulska-Wasilewska A., Fabianova E., Fucic A., Hagmar L., Joksic G., Martelli A., Migliore L., Mirkova E., Scarfi M.R., Zijno A., Norppa H., Fenech M. An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans. Carcinogenesis 2007, 28:625-631.
7. Nersesyan A.K. Possible role of the micronucleus assay in diagnostics and secondary prevention of cervix cancer: a minireview. Tsitol. Genet. 2007, 41:64-66.
8. Varga D., Vogel W., Bender A., Surowy H., Maier C., Kreienberg R., Deissler H., Sauer G. Increased radiosensitivity as an indicator of genes conferring breast cancer susceptibility. Strahlenther. Onkol. 2007, 183:655-660.
9. Evans H.J., Neary G.J., Williamson F.S. The relative biological efficiency of single doses of fast neutrons and gamma-rays on Vicia faba roots and the effect of oxygen. Part II. Chromosone damage: the production of micronuclei. Int. J. Radiat. Biol. 1959, 1:216-229.
10. Heddle J.A., Carrano A.V. The DNA content of micronuclei induced in mouse bone marrow by gamma-irradiation: evidence that micronuclei arise from acentric chromosomal fragments. Mutat. Res. 1977, 44:63-69.
11. Hayashi M., Sofuni T., Ishidate M. Kinetics of micronucleus formation in relation to chromosomal aberrations in mouse bone marrow. Mutat. Res. 1984, 127:129-137.
12. Norppa H., Falck G.C. What do human micronuclei contain?. Mutagenesis 2003, 18:221-233.
13. Saunders W.S., Shuster M., Huang X., Gharaibeh B., Enyenihi A.H., Petersen I., Gollin S.M. Chromosomal instability and cytoskeletal defects in oral cancer cells. Proc. Natl. Acad. Sci. U.S.A. 2000, 97:303-308.
14. Thomas P., Umegaki K., Fenech M. Nucleoplasmic bridges are a sensitive measure of chromosome rearrangement in the cytokinesis-block micronucleus assay. Mutagenesis 2003, 18:187-194.
15. Rao X., Zhang Y., Yi Q., Hou H., Xu B., Chu L., Huang Y., Zhang W., Fenech M., Shi Q. Multiple origins of spontaneously arising micronuclei in HeLa cells: direct evidence from long-term live cell imaging. Mutat. Res. 2008, 646:41-49.
16. Hoffelder D.R., Luo L., Burke N.A., Watkins S.C., Gollin S.M., Saunders W.S. Resolution of anaphase bridges in cancer cells. Chromosoma 2004, 112:389-397.
17. Rens W., Torosantucci L., Degrassi F., Ferguson-Smith M.A. Incomplete sister chromatid separation of long chromosome arms. Chromosoma 2006, 115:481-490.
18. Utani K., Kawamoto J.K., Shimizu N. Micronuclei bearing acentric extrachromosomal chromatin are transcriptionally competent and may perturb the cancer cell phenotype. Mol. Cancer Res. 2007, 5:695-704.
19. Labidi B., Gregoire M., Frackowiak S., Hernandez-Verdun D., Bouteille M. RNA polymerase activity in PtK1 micronuclei containing individual chromosomes. An in vitro and in situ study. Exp. Cell Res. 1987, 169:233-244.
20. Kato H., Sandberg A.A. Chromosome pulverization in human cells with micronuclei. J. Natl. Cancer Inst. 1968, 40:165-179.
21. Honma M., Hayashi M., Sofuni T. Cytotoxic and mutagenic responses to X-rays and chemical mutagens in normal and p53-mutated human lymphoblastoid cells. Mutat. Res. 1997, 374:89-98.
22. Honma M., Sakuraba M., Koizumi T., Takashima Y., Sakamoto H., Hayashi M. Non-homologous end-joining for repairing I-SceI-induced DNA double strand breaks in human cells. DNA Repair (Amst) 2007, 6:781-788.
23. Sugimoto K., Urano T., Zushi H., Inoue K., Tasaka H., Tachibana M., Dotsu M. Molecular dynamics of Aurora-A kinase in living mitotic cells simultaneously visualized with histone H3 and nuclear membrane protein importinalpha. Cell Struct. Funct. 2002, 27:457-467.
24. Sugimoto K., Senda-Murata K., Oka S. Construction of three quadruple-fluorescent MDA435 cell lines that enable monitoring of the whole chromosome segregation process in the living state. Mutat. Res. 2008, 657:56-62.
25. Kruskal B.A., Keith C.H., Maxfield F.R. Thyrotropin-releasing hormone-induced changes in intracellular [Ca2+] measured by microspectrofluorometry on individual quin2-loaded cells. J. Cell Biol. 1984, 99:1167-1172.
26. Matsushima T., Hayashi M., Matsuoka A., Ishidate M., Miura K.F., Shimizu H., Suzuki Y., Morimoto K., Ogura H., Mure K., Koshi K., Sofuni T. Validation study of the in vitro micronucleus test in a Chinese hamster lung cell line (CHL/IU). Mutagenesis 1999, 14:569-580.
27. Luan Y., Suzuki T., Palanisamy R., Takashima Y., Sakamoto H., Sakuraba M., Koizumi T., Saito M., Matsufuji H., Yamagata K., Yamaguchi T., Hayashi M., Honma M. Potassium bromate treatment predominantly causes large deletions, but not GC>TA transversion in human cells. Mutat. Res. 2007, 619:113-123.
28. Koyama N., Sakamoto H., Sakuraba M., Koizumi T., Takashima Y., Hayashi M., Matsufuji H., Yamagata K., Masuda S., Kinae N., Honma M. Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells. Mutat. Res. 2006, 603:151-158.
29. Sato N., Mizumoto K., Nakamura M., Ueno H., Minamishima Y.A., Farber J.L., Tanaka M. A possible role for centrosome overduplication in radiation-induced cell death. Oncogene 2000, 19:5281-5290.
30. Fukasawa K. Centrosome amplification, chromosome instability and cancer development. Cancer Lett. 2005, 230:6-19.
31. Uetake Y, Sluder G. Cell cycle progression after cleavage failure: mammalian somatic cells do not possess a " tetraploidy checkpoint" J. Cell Biol. 2004, 165:609-615.
32. Tomasz M. Mitomycin C: small, fast and deadly (but very selective). Chem. Biol. 1995, 2:575-579.
33. Ford J.H., Schultz C.J., Correll A.T. Chromosome elimination in micronuclei: a common cause of hypoploidy. Am. J. Hum. Genet. 1988, 43:733-740.