Molecular dynamics of Aurora-A kinase in living mitotic cells simultaneously visualized with histone H3 and nuclear membrane protein importinα

Cell Structure and Function

Volumn 27, Issue 6, 2002, Pages 457-467

  Sugimoto, Kenji ^a   Urano, Takeshi ^b   Zushi, Hitomi ^a   Inoue, Kimiko ^a   Tasaka, Hiroaki ^a   Tachibana, Makoto ^c   Dotsu, Masaya ^d  

^a Osaka Prefecture University   (Japan)

^b NAGOYA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c KYOTO UNIVERSITY   (Japan)

^d Mitani Corporation   (Japan)

Author keywords

Aurora A; Centrosome; GFP; Histone H3; Importin ; Timelapse

Indexed keywords

ALPHA TUBULIN; AURORA A KINASE; GAMMA TUBULIN; GREEN FLUORESCENT PROTEIN; HISTONE H3; HYBRID PROTEIN; KARYOPHERIN ALPHA; MEMBRANE PROTEIN; PROTEIN ANTIBODY; PROTEIN KINASE; RED FLUORESCENT PROTEIN; UNCLASSIFIED DRUG;

ANAPHASE; ARTICLE; CELL CYCLE G2 PHASE; CELL LINE; CELL NUCLEUS; CELL NUCLEUS MEMBRANE; CELL SHAPE; CELL SURFACE; CENTROSOME; CHROMATIN CONDENSATION; CHROMOSOME STRUCTURE; CHROMOSOME TRANSLOCATION; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME LOCALIZATION; ENZYME STRUCTURE; GENE CONSTRUCT; HUMAN; HUMAN CELL; IMAGING SYSTEM; IMMUNOHISTOCHEMISTRY; INTERPHASE; MEMBRANE FORMATION; MEMBRANE STRUCTURE; METAPHASE; MITOSIS; MITOSIS SPINDLE; MOLECULAR DYNAMICS; MOLECULAR STABILITY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROPHASE; PROTEIN EXPRESSION; SISTER CHROMATID; SPINDLE POLE BODY; TELOPHASE;

ALPHA KARYOPHERINS; CELL CYCLE PROTEINS; CELL LINE; CENTROSOME; CHROMATIN; EUKARYOTIC CELLS; HISTONES; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, VIDEO; MITOSIS; MITOTIC SPINDLE APPARATUS; NUCLEAR ENVELOPE; PROTEIN KINASES; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT FUSION PROTEINS; TUBULIN; XENOPUS PROTEINS;

EID: 0037005263     PISSN: 03867196     EISSN: None     Source Type: Journal    
DOI: 10.1247/csf.27.457     Document Type: Article

References (31)

1. Aagaard, L., Schmid, M., Warburton, P., and Jenuwein, T. 2000. Mitotic phosphorylation of SUV39H1, a novel component of active centromeres, coincides with transient accumulation at mammalian centromeres. J. Cell Sci., 113: 817-829.
2. Adams, R.R., Wheatley, S.P., Gouldsworthy, A.M., Kandels-Lewis, S.E., Carmena, M., Smythe, C., Gerloff, D.L., and Earnshaw, W.C. 2000. INCENP binds the Aurora-related kinase AIRK2 and is required to target it to chromosomes, the central spindle and cleavage furrow. Curr. Biol., 10: 1075-1078.
3. Baird, G.S., Zacharias, D.A., and Tsien, R.Y. 2000. Biochemistry, mutagenesis, and oligomerization of DsRed, a fluorescent protein from coral. Proc. Natl. Acad. Sci. USA, 97: 11984-11989.
4. Beaudouin, J., Gerlich, D., Daigle, N., Elis, R., and Ellenberg, J. 2002. Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina. Cell, 108: 83-96.
5. Berdnik, D. and Knoblich, J.A. 2002. Drosophila Aurora-A is required for centrosome maturation and actin-dependent asymmetric protein localization during mitosis. Curr. Biol., 12: 640-647.
6. Bischoff, J.R., Anderson, L., Zhu, Y., Mossie, K., Ng, L., Souza, B., Schryver, B., Flanagan, P., Clairvoyant, G., Ginther, C., Chan, C.S., Novotny, M., Salmon, D.J., and Plowman, G.D. 1998. A homolog of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers. EMBO J., 17: 3052-3065.
7. Bischoff, J.R. and Plowman, G.D. 1999. The Aurora/Ip11p kinase family: regulators of chromosome segregation and cytokinesis. Trends Cell Biol., 9: 454-459.
8. Brenner, S., Pepper, D., Berns, M.W., Tan, E.M., and Brinkley, B.R. 1981. Kinetochore structure, duplication, and distribution in mammalian cells: analysis by human autoantibodies from scleroderma patients. J. Cell Biol., 91: 95-102.
9. Chalfie, M., Tu, Y., Euskkirchen, G., Ward, W.W., and Prasher, D.C. 1994. Green fluorescent protein as a marker for gene expression. Science, 263: 802-805.
10. Chan, G.K.T., Schaar, B.T., and Yen, T.J. 1998. Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1. J. Cell Biol., 143: 49-63.
11. Crosio, C., Fimia, G.M., Loury, R., Kimura, M., Okano, Y., Zhou, H., Sen, S., Allis, C.D., and Sassone-Corsi, P. 2002. Mitotic phosphorylation of histone H3: spatio-temporal regulation by mammalian Aurora kinases. Mol. Cell Biol., 22: 874-885.
12. Finley, K.R., Davidson, A.E., and Ekker, S.C. 2001. Three-color imaging using fluorescent proteins in living zebrafish embryos. Biotechniques, 31: 66-72.
13. Giet, R. and Prigent, C. 1999. Aurora/Ip11p-related kinases, a new oncogenic family of mitotic serine-threonine kinases. J. Cell Sci., 112: 3591-3601.
14. Glover, D.M., Leibowitz, M.H., McLean, D.A., and Parry, H. 1995. Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles. Cell, 81: 95-105.
15. Goepfert, T.M. and Brinkley, B.R. 2000. The centrosome-associated Aurora/Ip1-like kinase family. Curr. Topics Develop. Biol., 49: 331-342.
16. Hannak, E., Kirkham, M., Hyman, A.A., and Oegema, K. 2001. Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans. J. Cell Biol. 155: 1109-1115.
17. Hooser, A.V., Goodrich, D.W., Allis, C.D., Brinkley, B.R., and Mancini, M.A. 1998. Histone H3 phosphorylation is required for the initiation, but not maintenance of mammalian chromosome condensation. J. Cell Sci., 111: 3497-3506.
18. Kimura, M., Kotani, S., Hattori, Sumi, N., Yoshioka, T., Todokoro, K., and Okano, Y. 1997. Cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, Aik, related Aurora of Drosophila and Yeast IPL1. J. Biol. Chem., 272: 13766-13771.
19. Lanlan, Y.W., Bowen, J., Gorovsky, M.A., and Allis, C.D. 1999. Phosphorylation of histone H3 is required for proper chromosome condensation and segregation. Cell, 97: 99-109.
20. Nigg, E.A. 2001. Mitotic kinases as regulators of cell division and its checkpoints. Nature Rev. Mol. Cell Biol., 2: 21-32.
21. Rogers, E., Bishop, J.D., Waddle, J.A., Schumacher, J.M., and Lin, R. 2002. The aurora kinase AIR-2 functions in the release of chromosome cohesion in Caenorhabditis elegans meiosis. J. Cell Biol., 157: 219-229.
22. Salina D., Bodoor, K., Eckley, D.M., Schoer, T.A., Rattner, J.B., and Burke, B. 2002. Cytoplasmic dynein as a facilitator of nuclear envelope breakdown. Cell, 108: 97-107.
23. Schumacher, J.M., Ashcroft, N., Donovan, P., and Golden, A. 1998. A highly conserved centrosomal kinase, AIR-1, is required for accurate cell cycle progression and segregation of developmental factors in Caenorhabditis elegans embryos. Development, 125: 4391-4402.
24. Scrittori, L., Hans, F., Angelov, D., Charra, M., Prigent, C., and Dimitrov, S. 2001. pEg2 Aurora-A kinase, histone H3 phosphorylation, and chromosome assembly in Xenopus egg extract. J. Biol. Chem., 276: 30002-30010.
25. Sugimoto, K., Fukuda, R., and Himeno, M. 2000. Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein. Cell Struct. Funct., 25: 253-261.
26. Sugimoto, K., Tasaka, H., and Dotsu, M. 2001. Molecular behavior in living mitotic cells of human centromere heterochromatin protein HP1 α ectopically expressed as a fusion to red fluorescent protein. Cell Struct. Funct., 26: 705-718.
27. Sugiyama, K., Sugiura, K., Hara, T., Sugimoto, K., Shima, H., Honda, K., Furukawa, K., Yamashita, S., and Urano, T. 2002. Aurora-B associated protein phosphatases as negative regulators of kinase activation. Oncogene, 21: 3103-3111.
28. Tachibana, M., Sugimoto, K., Fukushima, T., and Shinkai, Y. 2001. SET domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3. J. Biol. Chem., 276: 25309-25317.
29. Vig, B.K., Latour D., and Brown, N. 1996. Localization of anti-CENP antibodies and alphoid sequences in acentric heterochromatin in a breast cancer cell line. Cancer Genet. Cytogenet., 88: 118-125.
30. Zeitlin, S.G., Shelby, R.D., and Sullivan, K.F. 2001. CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis. J. Cell Biol., 155: 1147-1157.
31. Zhou, H., Kuang, J., Zhong, L., Kuo, W.L., Gray, J.W., Sahin, A., Brinkley, B.R., and Sen, S. 1998. Tumor amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation. Nat. Genet., 20: 189-193.