메뉴 건너뛰기




Volumn 33, Issue 3, 2010, Pages 215-222

A strategy to increase the specificity of Syber Green I qRT-PCR in hepatitis A detection

Author keywords

Hairpin primer; HAV; Primer dimers; QRT PCR; Specificity; Syber green I

Indexed keywords

FLUORESCENT DYE; PRIMER DNA;

EID: 77957064551     PISSN: 11217138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article
Times cited : (2)

References (31)
  • 1
    • 0033729713 scopus 로고    scopus 로고
    • Controlled hot start and improved specificity in carrying out PCR utilizing touch-up and loop incorporated primers (TULIPS)
    • AILENBERG M., SILVERMAN M. (2000). Controlled hot start and improved specificity in carrying out PCR utilizing touch-up and loop incorporated primers (TULIPS). Bio Techniques. 29, 1018-1024.
    • (2000) Bio Techniques. , vol.29 , pp. 1018-1024
    • Ailenberg, M.1    Silverman, M.2
  • 3
    • 20444448829 scopus 로고    scopus 로고
    • Detection and quantification of hepatitis A virus in seawater via real-time RT-PCR
    • DOI 10.1016/j.jviromet.2005.03.017, PII S0166093405000972
    • BROOKS H.A., GERSBERG R.M., DHAR A.K. (2005). Detection and quantification of hepatitis A virus in seawater via real-time RT-PCR. J. Virol. Methods. 127, 109-118. (Pubitemid 40828187)
    • (2005) Journal of Virological Methods , vol.127 , Issue.2 , pp. 109-118
    • Brooks, H.A.1    Gersberg, R.M.2    Dhar, A.K.3
  • 5
    • 35948964037 scopus 로고    scopus 로고
    • Evaluation of an extracting method for the detection of Hepatitis A virus in shellfish by SYBR-Green real-time RT-PCR
    • CASAS N., AMARITA F., MARTÍNEZ DE MARAÑÓN I. (2007). Evaluation of an extracting method for the detection of Hepatitis A virus in shellfish by SYBR-Green real-time RT-PCR. Int. J. Food Microbiol. 120, 179-185.
    • (2007) Int. J. Food Microbiol. , vol.120 , pp. 179-185
    • Casas, N.1    Amarita, F.2    De Martínez Marañón, I.3
  • 6
    • 33745152691 scopus 로고    scopus 로고
    • Development, evaluation and standardization of a real-time Taq-Man reverse transcription-PCR assay for the quantification of hepatitis A virus in clinical and shellfish samples
    • COSTAFREDA M.I., BOSCH A., PINTO R.M. (2006). Development, evaluation and standardization of a real-time Taq-Man reverse transcription-PCR assay for the quantification of hepatitis A virus in clinical and shellfish samples. Appl. Environ. Microbiol. 72, 3846-3855.
    • (2006) Appl. Environ. Microbiol. , vol.72 , pp. 3846-3855
    • Costafreda, M.I.1    Bosch, A.2    Pinto, R.M.3
  • 8
    • 0036904432 scopus 로고    scopus 로고
    • Quantification and duration of viraemia during hepatitis A infection as determined by real-time RTPCR
    • COSTA-MATTIOLI, M., MONPOEHO S., NICAND E., ALEMAN M.H., BILLAUDEL S., FERRE V. (2002 b). Quantification and duration of viraemia during hepatitis A infection as determined by real-time RTPCR. J. Viral. Hepat. 9, 101-106.
    • (2002) J. Viral. Hepat. , vol.9 , pp. 101-106
    • Costa-Mattioli, M.1    Monpoeho, S.2    Nicand, E.3    Aleman, M.H.4    Billaudel, S.5    Ferre, V.6
  • 10
    • 17644382240 scopus 로고    scopus 로고
    • Use of armored RNA as a standard to construct a calibration curve for real-time RT-PCR
    • DONIA D., DIVTZIA M., PANÀ A. (2005). Use of armored RNA as a standard to construct a calibration curve for real-time RT-PCR. J. Virol. Methods. 126, 157-163.
    • (2005) J. Virol. Methods. , vol.126 , pp. 157-163
    • Donia, D.1    Divtzia, M.2    Panà, A.3
  • 11
    • 50949086058 scopus 로고    scopus 로고
    • Problematiche connesse con l'applicazione della RT-PCR real-time nelle analisi ambientali
    • DONIA D., DIVIZIA M., PANÀ A. (2006). Problematiche connesse con l'applicazione della RT-PCR real-time nelle analisi ambientali. Ig. Sanità Pubbl. 62, 409-420.
    • (2006) Ig. Sanità Pubbl. , vol.62 , pp. 409-420
    • Donia, D.1    Divizia, M.2    Panà, A.3
  • 12
    • 20444382331 scopus 로고    scopus 로고
    • Development and evaluation of a broadly reactive TaqMan assay for rapid detection of hepatitis A virus
    • JOTHIKUMAR N., CROMEANS T.L., SOBSEY M.D., ROBERTSON B.H. (2005). Development and evaluation of a broadly reactive TaqMan assay for rapid detection of hepatitis A virus. Appl. Environ. Microbiol. 71, 3359-3363.
    • (2005) Appl. Environ. Microbiol. , vol.71 , pp. 3359-3363
    • Jothikumar, N.1    Cromeans, T.L.2    Sobsey, M.D.3    Robertson, B.H.4
  • 13
    • 0034320328 scopus 로고    scopus 로고
    • PCR hot start with primers with the structure of molecular beacons (hairpinlike structure)
    • KABOEV O.K., LUCHKINA L.A., TRET'IAKOV A.N., BAHRMAND A.R. (2000). PCR hot start with primers with the structure of molecular beacons (hairpinlike structure). Nucleic Acids Res. 28, E94.
    • (2000) Nucleic Acids Res. , vol.28
    • Kaboev, O.K.1    Luchkina, L.A.2    Tret'Iakov, A.N.3    Bahrmand, A.R.4
  • 14
    • 5444223470 scopus 로고    scopus 로고
    • Characterization of the complete genomic sequence of genotype II hepatitis A virus (CF53/Berne isolate)
    • DOI 10.1099/vir.0.80304-0
    • Lu L., CHING K.Z., DE PAULA V.S., NAKANO T., SIEGL G., WEITZ M., ROBERTSON E.H. (2004). Characterization of the complete genomic sequence of genotype II hepatitis A virus (CF53/Berne isolate). J. Gen. Virol. 85, 2943-2952. (Pubitemid 39360038)
    • (2004) Journal of General Virology , vol.85 , Issue.10 , pp. 2943-2952
    • Lu, L.1    Ching, K.Z.2    De Paula, V.S.3    Nakano, T.4    Siegl, G.5    Weitz, M.6    Robertson, B.H.7
  • 15
    • 33244497831 scopus 로고    scopus 로고
    • Identification and sequence analysis of hepatitis A virus detected in market and environmental bivalve molluscs
    • MACALUSO A., PETRINCA A., LANNI L., SACCARES S., AMITI S., GABRIELI R., DIVIZIA M. (2006). Identification and sequence analysis of hepatitis A virus detected in market and environmental bivalve molluscs. J. Food Prot. 69 (2), 449-452.
    • (2006) J. Food Prot. , vol.69 , Issue.2 , pp. 449-452
    • Macaluso, A.1    Petrinca, A.2    Lanni, L.3    Saccares, S.4    Amiti, S.5    Gabrieli, R.6    Divizia, M.7
  • 17
    • 31544445806 scopus 로고    scopus 로고
    • Diagnosis of hepatitis A virus infection: A molecular approach
    • DOI 10.1128/CMR.19.1.63-79.2006
    • NAINAN O.V, XIA G., VAUGHAN G., MARGOUS H.S. (2006). Diagnosis of hepatitis A virus infection: a molecular approach. Clin. Microbiol. Rev. 19, 63-79. (Pubitemid 43157647)
    • (2006) Clinical Microbiology Reviews , vol.19 , Issue.1 , pp. 63-79
    • Nainan, O.V.1    Xia, G.2    Vaughan, G.3    Margolis, H.S.4
  • 18
    • 0036567249 scopus 로고    scopus 로고
    • Effect of primary and secondary structure of oligodeoxyribonucleotides on the fluorescent properties of conjugated dyes
    • NAZARENKO I., PIRES K., LOWE B., OBAIDY M., RASHTCHIAN A. (2002a). Effect of primary and secondary structure of oligodeoxyribonucleotides on the fluorescent properties of conjugated dyes. Nucleic Acids Res. 30, 2089-2195.
    • (2002) Nucleic Acids Res. , vol.30 , pp. 2089-2195
    • Nazarenko, I.1    Pires, K.2    Lowe, B.3    Obaidy, M.4    Rashtchian, A.5
  • 22
    • 0031568919 scopus 로고    scopus 로고
    • Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
    • DOI 10.1006/abio.1996.9916
    • RIRIE K.M., RASMUSSEN R.T., WITTWER C.T. (1997). Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal. Biochem. 245, 154-160. (Pubitemid 27082244)
    • (1997) Analytical Biochemistry , vol.245 , Issue.2 , pp. 154-160
    • Ririe, K.M.1    Rasmussen, R.P.2    Wittwer, C.T.3
  • 24
    • 0003004307 scopus 로고
    • Selection of primers for polymerase chain reaction
    • Editor: White BA.Totowa, New Jersey: Humana Press, Chapter. 2, 31-40
    • RYCHLIK W. (1993). Selection of primers for polymerase chain reaction. In: PCR Protocols. Current Methods and Applications. Editor: White BA.Totowa, New Jersey: Humana Press, Chapter. 2, 31-40.
    • (1993) PCR Protocols Current Methods and Applications.
    • Rychlik, W.1
  • 25
    • 32344451349 scopus 로고    scopus 로고
    • Detection and differentiation of human hepatitis A strains by commercial quantitative real-time RT-PCR tests
    • SÁNCHEZ G., POPULAIRE S., BUTOT S., PUTALLAZ T., JOOSTEN H. (2006). Detection and differentiation of human hepatitis A strains by commercial quantitative real-time RT-PCR tests J. Virol. Methods. 132, 160-165.
    • (2006) J. Virol. Methods. , vol.132 , pp. 160-165
    • Sánchez, G.1    Populaire, S.2    Butot, S.3    Putallaz, T.4    Joosten, H.5
  • 26
    • 34347360835 scopus 로고    scopus 로고
    • Hepatitis A virus detection in food: Current and future prospects
    • SÁNCHEZ G., BOSCH A., PINTÒ R.M. (2007). Hepatitis A virus detection in food: current and future prospects. Letters in Appl. Microbiol. 45, 1-5.
    • (2007) Letters in Appl. Microbiol. , vol.45 , pp. 1-5
    • Sánchez, G.1    Bosch, A.2    Pintò, R.M.3
  • 28
    • 3943088401 scopus 로고    scopus 로고
    • AutoDimer: A screening tool for primer-dimer and hairpin structures
    • VALLONE P.M., BUTLER J.M. (2004). AutoDimer: a screening tool for primer-dimer and hairpin structures. BioTechniques. 37, 226-231.
    • (2004) BioTechniques. , vol.37 , pp. 226-231
    • Vallone, P.M.1    Butler, J.M.2
  • 29
    • 0036534582 scopus 로고    scopus 로고
    • Elimination of primer-dimer artifacts and genomic coamplification using a two-step SYBR Green i real-time RT-PCR
    • VANDESOMPELE J., DE PAEPE A.,D SPELEMAN F. (2002). Elimination of primer-dimer artifacts and genomic coamplification using a two-step SYBR Green I real-time RT-PCR. Analytical Biochemistry. 303, 95-98.
    • (2002) Analytical Biochemistry. , vol.303 , pp. 95-98
    • Vandesompele, J.1    De Paepe, A.2    Speleman, F.3
  • 31
    • 33748702280 scopus 로고    scopus 로고
    • Evaluation of methods used to concentrate and detect hepatitis A virus in water samples
    • VILLAR L.M., DE PAULA VS., DINIZ-MENDES L., LAMPE E. AND GASPAR A.M. (2006) Evaluation of methods used to concentrate and detect hepatitis A virus in water samples. J. Virol. Methods. 137, 169-176.
    • (2006) J. Virol. Methods. , vol.137 , pp. 169-176
    • Villar, L.M.1    De Paula, V.S.2    Diniz-Mendes, L.3    Lampe, E.4    Gaspar, A.M.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.