Quantification and duration of viraemia during hepatitis a infection as determined by real-time RT-PCR

Journal of Viral Hepatitis

Volumn 9, Issue 2, 2002, Pages 101-106

  Costa Mattioli, M ^a   Monpoeho, S ^a   Nicand, E ^b   Aleman, M H ^a   Billaudel, S ^a   Ferre V ^a  

^a NANTES UNIVERSITY HOSPITAL   (France)

^b University of Paris Descartes   (France)

Author keywords

HAV; RNA; RT PCR quantification; TaqMan; Viraemia

Indexed keywords

IMMUNOGLOBULIN M; VIRUS PROTEIN;

ADOLESCENT; ADULT; ARTICLE; BLOOD ANALYSIS; CLINICAL ARTICLE; CONTROLLED STUDY; DISEASE DURATION; FEMALE; HEPATITIS A; HUMAN; MALE; PRESCHOOL CHILD; PRIORITY JOURNAL; QUANTITATIVE DIAGNOSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCHOOL CHILD; VIREMIA; VIRUS DIAGNOSIS;

ADOLESCENT; ADULT; CHILD; FEMALE; HEPATITIS A; HEPATITIS A VIRUS, HUMAN; HUMANS; MALE; MIDDLE AGED; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; TIME FACTORS; VIREMIA;

EID: 0036904432     PISSN: 13520504     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2893.2002.00336.x     Document Type: Article

References (20)

1. Matthews REF. Classification and nomenclature of virus. Fourth report of the International Committee on Taxonomy of Virus. Intervirology 1982; 17: 1-200.
2. Melnick JL. Classification of hepatitis A virus as enterovirus type 72 and of hepatitis B virus as hepadnavirus type 1. Intervirology 1982; 18: 105-106
3. Fujiwara K, Yokosuka O, Ehata T et al. Frequent detection of hepatitis A viral RNA in serum during the early convalescent phase of acute hepatitis A. Hepatology 1997; 26: 1634-1639.
4. Kedda MA, Kew MC, Cohn RJ et al. An outbreak of hepatitis A among South African patients with hemophilia: Evidence implicating contaminated factor VIII concentrate as the source. Hepatology 1995; 22: 1363-1367.
5. Chudy M, Budek I, Keller-Stanislawski B et al. A new cluster of hepatitis A infection in hemophiliacs traced to a contaminated plasma pool. J Med Virol 1999; 57: 91-99.
6. Cohen JI, Ticehurst JR, Purcell RH, Bouckler-White A, Baroudy BM. Complete nucleotide sequence of wild type hepatitis A virus comparison with different strains of hepatitis A virus and other picornavirus. J Virol 1987; 61: 50-59.
7. Jansen RW, Newbold JE, Lemon S. Complete nucleotide sequence of a cell culture adapted variant of hepatitis A virus: Comparison with wild-type virus with restricted capacity for in vitro replication. Virology 1988; 163: 299-307.
8. Repp R, Borkhart A, Gassen RK, Hammerman J, Lambert C. Construction of RNA standard for high-resolution automatic product analysis in quantitative competitive RT-PCR. Biotechniques 1995; 19: 84-90.
9. Arnal C, Ferre-Aubineau V, Mignotte B, Imbert MB, Billaudel S. Quantification of hepatitis A virus in shellfish by competitive reverse transcription-PCR with coextraction of standard RNA. Appl Environ Microbiol 1999; 65: 322-326.
10. Monpoeho S, Dehée A, Mignotte B et al. Quantification of enterovirus RNA in sludge samples by single tube real-time RT-PCR. Biotechniques 2000; 29: 88-93.
11. Martell M, Gomez J, Esteban JI et al. High-throughput real-time reverse transcription-PCR quantitation of hepatitis C virus RNA. J Clin Microbiol 1999; 37: 327-332.
12. Flehmig B, Heinarcy U, Pfisterer M. Prospect for a hepatitis A vaccine. J Med Virol 1991; 37: 47-55.
13. Provost PJ, Hilleman MR. Propagation of human hepatitis A virus in cell culture in vitro. Proc Soc Exp Biol Med 1979; 160: 213-221.
14. Desjardin LE, Chen Y, Perkins MD, Teixeira L, Cave MD, Eisenach KD. Comparison of the ABI 7700 system (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of tuberculosis. J Clin Microbiol 1998; 36: 1964-1968.
15. Raeymaekers L. Quantitative PCR. Theoretical considerations with practical implications. Anal Biochem 1993; 214: 582-585.
16. Gelmini S, Orlando C, Sestini R et al. Quantitative polymerase chain reaction-based homogeneous assay with measure c-erbB-2 oncogene amplification. Clin Chem 1997; 43: 52-58.
17. Chandler DP, Wagnon CA, Bolton H Jr. Reverse trascriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR. Appl Environ Microbiol 1998; 64: 669-677.
18. Cohen JI, Feinstone SM, Purcell RH. Hepatitis A virus infection in a chimpanzee. Duration of viremia, and detection of virus in saliva and throat swabs. J Infectious Dis 1989; 60: 887-890.
19. Lemon SM. The natural history of hepatitis A. The potential for transmission by transfusion of blood or blood products. Vox Sanguinis 1994; 4: 19-23.
20. Bower WA, Nainan OV, Margolis HS. Duration of viremia in hepatitis A virus infection. J Infectious Dis 2000; 182: 12-17.