Enzyme production in industrial fungi- molecular genetic strategies for integrated strain improvement

Applied Mycology and Biotechnology

Volumn 3, Issue C, 2003, Pages 241-259

  Helena Nevalainen, K M ^a   S Jnr Te'o, Valentino ^a  

^a MACQUARIE UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 77957037345     PISSN: 18745334     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S1874-5334(03)80014-X     Document Type: Chapter

References (123)

1. Agger T., and Nielsen J. Genetically structured modeling of protein production in filamentous fungi. Biotechnol Bioeng 66 (1999) 164-170
2. Archer D.B., and Peberdy J.F. The molecular biology of secreted enzyme production by filamentous fungi. Crit Rev Biotechnol 17 (1997) 73-306
3. Arnold F.H. Directed Evolution: Creating Biocatalysts For The Future. Chem Eng Sci 51 (1996) 5091-5102
4. Aro N., Saloheimo A., Ilmèn M., and Penttilä M. CEEII, a novel transcriptional activator involved in the regulation of cellulase and xylanase genes of Trichoderma reesei. J Biol. Chem. 276 (2001) 24309-24314
5. Aro N., Ilmén M., Saloheimo A., and Penttilä M. ACEI of Trichoderma reesei is a repressor of cellulase and xylanase expression (2002), Appl Environ Microbiol in press
6. Azmi O.R., and Seppelt R.D. The broad distribution of microfungi in the Windmill Islands region, continental Antarctica. Polar Biol 19 (1998) 92-100
7. Baker D.A., Shiau A.K., and Agard D.A. The role of pro regions in protein folding. Curr Opin Cell Biol 5 (1993) 966-970
8. WO 90/00192. Berka R., Hayenga K., Lawlis V.B., and Ward M. Aspartic proteinase deficient filamentous fungi (1990)
9. Berka R.M., Ward M., Wilson L.J., Hayenga K.J., Kodama K.H., Carlomagno L.P., and Thompson S.A. Molecular cloning and deletion of the gene encoding aspergillopepsin A fromAspergillus awamori. Gene. 86 (1990) 153-162
10. Blinkovsky A.M., Buyn T., Brown K.M., and Golitghly E. Purification, characterization and heterologous expression in Fusarium venenatum of a novel serine carboxypeptidase from Aspergillus oryzae. Appl Environ Microbiol 65 (1999) 3298-3303
11. EP 0305216. Boel E., and Huge J.I.B. Recombinant Humicola lipase and process for the production of recombinant Humicola lipases European patent application (1989)
12. Bradner J.R., Gillings M., and Nevalainen H. Qualitative assessment of hydrolytic activities in antarctic fungi at different temperatures on solid media. World. J Microbiol Biotechnol 15 (1999) 143-145
13. Bradner J.R., Sidhu R.K., Gillings M., and Nevalainen H. Hemicellulase activity of antarctic microfungi. J Appl Microbiol 87 (1999) 366-370
14. Bradner J.R., and Nevalainen H. Metabolic activity in filamentous fungi can be analysed by flow cytometry. J Microbiol Meth (2002)
15. Brookman J.L., and Denning D.W. Molecular genetics ofAspergillus fumigatus. Curr Opin Biotechnol 3 (2000) 468-474
16. Buller A. Researches in Fungi (1933)
17. Caddick M.X., Brownlee A.G., and Arst Jr. H.N. Regulation of gene expression by pH of the growth medium inAspergillus nidulans. Mol Gen Genet 203 (1986) 346-353
18. Calmels T.P.G., Martin F., Durand H., and Tiraby G. Proteolytic events in the processing of secreted proteins in fungi. J Biotechnol 17 (1991) 51-66
19. Chambergo F.S., Bonaccorsi E.D., Ferreira A., Ramos A., Ribamar Jr. F., Abrahao-Neto J., Simon F.J., and El-Dorry H. Elucidation of the metabolic fate of glucose in the filamentous fungus Trichoderma reesei using EST analyisi and cDNA microarrays. J Biol Chem 277 (2002) 13983-13988
20. Chang S.C., Chang P.C., and Lee Y.H. The role of propeptide in maturation and secretion of Npr protease from Streptomyces. J Biol Chem 269 (1994) 3548-3554
21. Chapman R., Sidrauski C., and Walter P. Intracellular signalling from the endoplasmic reticulum to the nucleus. Annu Rev Cell Dev Biol 14 (1998) 459-485
22. Chaveroche M.K., Ghigo J.M., and d'Enfert C. A rapid method for efficient gene replacenment in the filamentous fungus Aspergillus nidulans. Nucleic Acids Res 28 (1900) E97
23. Chiba Y., Yamagata Y., Iijima S., Nakajima T., and Ichishima E. The carbohydrate moiety of the acid carboxypeptidase fromAspergillus saitoi. Curr Microbiol 27 (1993) 281-288
24. Christensen T., Hynes M.J., and Davis M.A. Role of the regulatory gene areA of Aspergillus oryzae in nitrogen metabolism. Appl Environ Microbiol 64 (1998) 3232-3237
25. Conesa A., van d.H.C., and Punt P. Studies on the production of fungal peroxidases in Aspergillus niger. Appl Environ Microbiol 66 (2000) 3016-3023
26. Conesa A., Punt P.J., van L.N., and van d.H.C. The secretion pathway in filamentous fungi: a biotechnological view. Fung Genet Biol 33 (2001) 155-171
27. Contreras R., Carrez D., Kinghorn J.R., van d.H.C., and Fiers W. Efficient KEX2-like processing of a glucoamylase-interleukin-6 fusion protein by Aspergillus nidulans and secretion of mature interleukin-6. Bio/Technology 9 (1991) 378-381
28. Cubero B., and Scazzocchio C. Two different, adjacent and divergent zinc finger binding sites are necessary for CREA-mediated carbon cataboliterepression in the proline gene cluster ofAspergillus nidulans. Embo J 13 (1994) 407-415
29. th European Conference on Fungal Genetics (2002)
30. Dalboge H. Expression cloning of fungal enzyme genes; a novel approach for efficient isolation of enzyme genes of industrial relevance. FEMS Microbiol Rev 1 (1997) 29-42
31. Dowzer C.E., and Kelly J.M. Analysis of the creA gene, a regulator of carbon catabolite repression in Aspergillus nidulans. Mol Cell Biol 11 (1991) 5701-5709
32. Drysdale M.R., Kolze S.E., and Kelly J.M. The Aspergillus niger carbon catabolite repressor encoding gene, creA. Gene 130 (1993) 241-245
33. Eriksson K.-E. Biotechnology in the pulp and paper industry: An overview. ACS Symp Ser 687 (1997) 2-14
34. Academic Press. Felenbok B., Flipphi M., and Nikolaev I. Ethanol catabolism in Aspergillus nidulans:a model for studying gene regulation. Progress in Nucleic Acid Research and Molecular Biology Vol 69 (2001) 149-204
35. Faria F.P., Te'o V.J.S., Bergquist P.L., Azevedo M.O., and Nevalainen K.M.H. Expression and processing of a major xylanase (XYN2) from the thermophilic fungus Humicola grisea var thermoidea in Trichoderma reesei. Lett Appl.Microbiol 34 (1902) 119-123
36. Farinas E.T., Bulter T., and Arnold F.H. Directed enzyme evolution. Curr Opin Biotechnol 12 (2001) 545-551
37. Finkelstein D.B. Transformation. DB Finkelstein and C Ball, eds. Biotechnology of Filamentous Fungi, Technology and Products (1992), MA: Butterworth-Heinemann, New York: Hafner 113-156
38. Fryksdale B.G., Jedrzejewski P.T., Wong D.L., Gaertner A.L., and Miller B.S. Impact of deglycosylation methods on two-dimensional gel electrophoresis and matrix assisted laserdesorption/ionization-time of flight-mass spectrometry for proteomic analysis. Electrophoresis 23 (1902) 2184-2193
39. Gebier J., Gilkes N.R., Claeyssens M., Wilson D.B., Beguin P., Wakarchuk W.W., Kilburn D.G., Miller Jr. R.C., Warren R.A., and Withers S.G. Stereoselective hydrolysis catalyzed by related beta-l,4-glucanases and beta-1,4-xylanases. J Biol Chem 267 (1992) 12559-12561
40. Gibbs M.J., Nevalainen K.M.H., and Bergquist P.L. Degenerate oligonucleotide gene shuffling (DOGS) : A method for enhancing the frequency of recombination with family shuffling. Gene 271 (1901) 13-20
41. Gielkens M., Gonzalez-Candelas L., Sanchez-Torres P., van d.V.P., de G.L., Visser J., and Ramon D. The abfB gene encoding the major alpha-L-arabinofuranosidase ofAspergillus nidulans: nucleotide sequence, regulation and construction of a disrupted. Microbiology 145 (1999) 735-741
42. Gielkens M.M., Dekkers E., Visser J., and de G.H. Two cellobiohydrolase-encoding genes from Aspergillus niger require D-xylose and the xylanolytic transcriptional activator XlnR for their expression. Appl Environ Microbiol 65 (1999) 4340-4345
43. Goller S.P., Schoisswohl D., Baron M., Parriche M., and Kubicek C.P. Role of endoproteolytic dibasic proprotein processing in maturation of secretory proteins in Trichoderma reesei. Appl Environ Microbiol 64 (1998) 3202-3208
44. Gordon C.L., Archer D.B., Jeenes D.J., Doonan J.H., Wells B., Trinci A., and Robson G.D. A glucoamylase:GFP gene fusion to study protein secretion by individual hyphae of Aspergillus niger. J Microbiol Methods 42 (1900) 39-48
45. Gordon C., Thomas S., Griffen A., Robinson G.D., Trinci P.J., and Wiebe M.G. Combined use of growth rate correlated and growth rate independent promoters for recombinant glucoamylase production in Fusarium venenatum. FEMS Microbiol Lett 194 (1901) 229-234
46. Gouka R.J., Punt P.J., and van d.H.CA. Efficient production of secreted proteins by Aspergillus: progress, limitations and prospects. Appl Microbiol Biotechnol 47 (1997) 1-11
47. Harkki A., Uusitalo J., Bailey M., Penttilä M., and Knowles J. A novel fungal expression system: secretion of active calf chymosin from the filamentous fungus Trichoderma reesei. Bio/Technol 7 (1989) 596-603
48. Harkki A., Mäntylä A., Muttilainen S., Bühler R., Suominen P., Knowles J., and Nevalainen H. Genetic engineering of Trichoderma to produce strains with novel cellulase profiles. Enzyme Microb Technol 13 (1991) 227-233
49. Harrison M.J., Nouwens A.S., Jardine D.R., Zachara N.E., Gooley A.A., Nevalainen H., and Packer N.H. Glycosylation of cellobiohydrolase I from Trichoderma reesei. Eur J Biochem 256 (1998) 119-127
50. Harrison M.J., Wathugala I.M., Tenkanen M., Packer N.H., and Nevalainen K.M.H. Glycosylation of acetylxylan esterase from Trichoderma reesei. Glycobiology 12 (2002) 291-298
51. Ilmén M., Thrane C., and Penttilä M. The glucose repressor gene crel of Trichoderma: isolation and expression of a full-length and a truncated mutant form. Mol Gen Genet 251 (1996) 451-460
52. Ilmén M. Molecular mechanisms of glucose repression in the filamentous fungus Trichoderma reesei (1997), VTT Publications 315, Espoo, Finland
53. Jalving R., van d.V.P., Visser J., and Schaap P.J. Characterization of the kexin-like maturase ofAspergillus niger. Appl Environ Microbiol 66 (2000) 363-368
54. Joern J.M., Meinhold P., and Arnold F.H. Analysis of shuffled gene libraries. J Mol Biol. 316 (2002) 643-656
55. Jeenes D.J., Mackenzie D.A., Roberts I.N., and Archer D.B. Heterologous protein production by filamentous fungi. Biotechnol Genet Eng Rev 9 (1991) 327-367
56. Jeenes D.J., MacKenzie D.A., and Archer D.B. A truncated glucoamylase gene fusion for heterologous protein secretion from Aspergillus niger. FEMS Microbiol Lett 107 (1993) 267-271
57. Karhunen T., Mäntylä A., Nevalainen H., and Suominen P. High frequency one-step gene replacement in Trichoderma reesei I. Endoglucanase I overproduction. Mol Gen Genet 241 (1993) 515-522
58. Klarskov K., Piens K., Stáhlberg J., Høi P.B., van B.J., and Claeyssens M. Cellobiohydrolase I from Trichoderma reesei: identification of an active-site nucleophile and additionalinformation on sequence including glycosylation pattern of the core protein. Carbohydr Res 304 (1997) 143-154
59. Kolkman J.A., and Stemmer W.P. Directed evolution of proteins by exon shuffling. Nat Biotechnol 19 (2001) 423-428
60. Krasevec N., van d.H.C., and Komel R. Can hTNF-alpha be successfully produced and secreted in filamentous fungusAspergillus niger?. Pflugers Arch 439 3 Supp1 (2000) R84-R86
61. Suppl. Lange L., Skjøt M., Schülein K.P., and Kauppinen S. Cellulase discovery and 18sDNA studies of five cythrids. Fungal Genetics Newsletter 46 (1999) p 56
62. Leisola M., Jokela J., Pastinen O., Turunen O., and Shoemaker H. Industrial use of enzymes (1902), INDUSTRIAL_USE_OF_ENZYMES.DOC. http://www.hut.fi/Units/Biotechnology/Kem
63. Liebeton K., Zonta A., Schimossek K., Nardini M., Lanf D., Dijkstra B.W., Reetz M., and Jaeger K.-E. directed evolution of an enantioselective lipase. Chem Biol 7 (1900) 709-718
64. Lim D., Hains P., Walsh B., Bergquist P., and Nevalainen H. Proteins associated with the cell envelope of Trichoderma reesei: A proteomic approach. Proteomics 1 (1901) 899-910
65. Lis H., and Sharon N. Protein glycosylation Structural and functional aspects. Eur J Biochem 218 (1993) 1-27
66. Liu F., Li W., Ridgway D., Gu T., and Moo-Young M. Inhibition of extracellular protease secretion by Aspergillus niger using immobilization. Biotechnol Lett 20 (1998) 539-542
67. Lorang J.M., Tuori R.P., Martinez J.P., Sawye T.L., Redman R.S., Rollins J.A., Wolpert T.J., Johnson K.B., Rodriguez R.J., Dickman M.B., and Ciuffetti L.M. Green fluorescent protein is lighting up fungal biology. Appl Environ Microbiol 67 (1901) 1987-1994
68. MacCabe A.P., Orejas M., Perez-Gonzalez J.A., and Ramon D. Opposite patterns of expression of two Aspergillus nidulans xylanase genes with respect to ambient pH. J Bacteriol 180 (1998) 1331-1333
69. Maheswari R., Bharadwaj G., and Bhat M.K. Thermophilic fungi: their physiology and enzymes. Microbiol Mol Biol Revs 64 (1900) 461-488
70. Mathieu M., and Felenbok B. The Aspergillus nidulans CREA protein mediates glucose repression of the ethanol regulon at various levels through competition with the AlcR-specific tran. EMBO J 13 (1994) 4022-4027
71. Maras M., de B.A., Schraml J., Herdewijn P., Claeyssens M., Fiers W., and Conteras W. Structural characterization of N-linked oligosaccharides from cellobiohydrolase I secreted by the filamentous fungus Trichoderma reesei RUTC 30. Eur J Biochem 245 (1997) 617-625
72. Maras M., De B.A., Schraml J., Herdewijn P., Piens K., Claeyssens M., Uusitalo J., Penttilä M., Fiers W., and Contreras R. Engineering of the carbohydrate moiety of fungal proteins to a mammalian type. In: Claeyssens M., Nerinckx W., and Piens K. (Eds). Carbohydrates from Trichoderma reesei and other microorganisms. Structures, biochemistry, genetics and applications (1997), The Royal Society of Chemistry, Cambridge UK 323-326
73. Maras M., De B.A., Vervecken W., Uusitalo J., Penttilä M., Busson R., Herdewijn P., and Contreras R. In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei. FEBS Lett 452 (1999) 365-370
74. Martin S.L., Blackmon B.P., Rajagopalan R., Houftek T.D., Sceeles R.G., Denn S.O., Mitchell T.K., Brown D.E., Wing R.A., and Dean R.A. MagnaportheDB: a federated solution for integrating physical and genetic map data with BAC and derived sequences for the rice blast fungus Magnaporthe grisea. Nucleic Acids Res 30 (1902) 121-124
75. Marvi J., Tanaka A., Mimura S., de G.L., Visser J., Kitamoto N., Kato M., Kobayashi T., and Tsukagoshi N. A transcriptional activator, AoXlnR, controls the expression of genes encoding xylanolytic enzymes in Aspergillus oryzae. Fung Genet Biol 35 (1902) 157-169
76. Melin P., Schnürer J., and Wagner E. Proteome analysis of Aspergillus nidulans reveals proteins associated with the response to the antibiotic concanamycin A, produced by Streptomyces species. Mol Gen Genom 267 (1902) 702-965
77. Mikosch M., Klemm P., Gassen H.G., van d.H.C., and Kemme M. Secretion of active human mucus propteinase inhibitor by Aspergillus niger after KEX2-like processing of a glucoamylase inhibitor fusion protein. J Biotechnol 52 (1996) 97-106
78. Moralejo F.J., Cardoza R.E., Gutierrez S., and Martin J.F. Thaumatin production inAspergillus awamori by use of expression cassettes with strong fungal promoters and high gene dosage. Appl Environ Microbiol 65 (1999) 1168-1174
79. Moralejo F.J., Watson A.J., Jeenes D.J., Archer D.B., and Martin J.F. A defined level of protein disulfide isomerase expression is required for optimal secretion of thaumatin by Aspergillus awamori. Mol Genet Genomics 266 (1901) 246-253
80. Montenecourt B.S., Nhlapo S.D., Trimino-Vazquez H., Cuskey H., Schamhart D.H.J., and Eveleigh D.E. Regulatory controls in relation to overproduction of fungal cellulases. In: Hollaender A., Rabson R., Rogers P., san Pietro A., Valentine R., and Wolfe R. (Eds). Trends in the Biotechnology of Fermentations for Fuels and Chemicals (1981), Plenum Publishing, New York 33-53
81. Morris D.D., Gibbs M.D., Chin C.W., Koh M.H., Wong K.K., Allison R.W., Nelson P.J., and Bergquist P.L. Cloning of the xynB gene from Dictyoglomus thermophilum Rt46B.l and action of the gene product on kraft pulp. Appl Environ Microbiol 64 (1998) 1759-1765
82. Mäntylä A., Saarelainen R., Fagerström R., Suominen P., and Nevalainen H. Cloning of the aspartic protease gene from Trichoderma reesei. 2nd European Conference on Fungal Genetics (1994)
83. Mäntylä A., Paloheimo M., and Suominen P. Industrial mutants and recombinant strains of Trichoderma reesei. In: Harman G.R., and CP K. (Eds). Enzymes, biological control and commercial applications. Trichoderma & Gliocladium Vol 2 (1998), Taylor and Francis, London 291-309
84. WO94/03612. Nevalainen H., Paloheimo M., Miettinen-Oinonen A., Torkkeli T., Turunen M., Fagerström R., Cantrell M., Piddington C., and Rambosek J. Production of phytate degrading enzymes in Trichoderma (1994)
85. in press. Nevalainen H., Te'o V., and Penttilä M. Application of genetic engineering for strain improvement in filamentous fungi (2002), Marcel Dekker, New York
86. Nykänen M., Saarelainen R., Raudaskoski M., Nevalainen H., and Mikkonen A. Expression and secretion of barley cysteine endopeptidase B and cellobiohydrolase I in Trichoderma reesei. Appl Environ Microbiol 63 (1997) 4929-4937
87. Nykänen M., Raudaskoski M., Nevalainen H., and Mikkonen A. Maturation of barley cysteine endopeptidase expressed in Trichoderma reesei is distorted by incomplete processing. Can J Microbiol 48 (2002) 138-150
88. Nykänen M. Protein secretion in Trichoderma reeseiExpression secretion and maturation of cellobiohydrolase I, barley cysteine endoproteinase and calf chymosin in Rut. PhD Dissertation (2002), University of Jyväskylä, Finland.
89. Nyyssönen E., Penttilä M., Harkki A., Saloheimo A., Knowles J.K.C., and Keränen S. Efficient production of antibody fragments by the filamentous fungus Trichoderma reesei. Bio/Technology 11 (1993) 591-595
90. Nyyssönen E., and Keränen S. Multiple roles of the cellulase CBHI in enhancing production of fusion antibodies by the filamentous fungus Trichoderma reesei. Curr Genet 28 (1995) 71-79
91. Paloheimo M., Mäntylä A., Vehmaanperä J.H.S., Lantto R., Lahtinen T., Parkkinen E., Fagerström R., and Suominen P. Thermostable xylanases produced by recombinant Trichoderma reesei for pulp bleaching. In: Claeyssens M., Nerinckx W., and Piens K. (Eds). Carbohydrates from Trichoderma reesei and other microorganisms. Structures, biochemistry, genetics and applications (1998), The Royal Society of Chemistry, Cambridge UK 255-264
92. Peek K., Ruttersmith L.D., Daniel R.M., Morgan H.W., and Bergquist P.L. Thermophilic enzymes as industrial catalysts. Int J Biotechnol 9 (1992) 466-470
93. Penttilä M. Heterologous protein production in Trichoderma. Enzymes, biological control and commercial applications Vol 2 (1998), Taylor and Francis, London 365-382 GR Harman and CP Kubicek
94. Petersen K.L., Lehmbeck J., and Christensen T. A new transcriptional activator for amylase genes in Aspergillus. Mol Gen Genet 262 (1999) 668-676
95. Roe B.A., Kupfer D., Zhu H., Gray J., Clifton S., Prade R., Loros J., and Dunlap J. Aspergillus nidulans (1999). http://www.genome.ou.edu/fungal.html.
96. Saloheimo A., Henrissat B., Hoffrén A.-M., Teleman O., and Penttilä M. A novel, small endoglucanase gene, eg/5, from Trichoderma reesei isolated by expression in yeast. Mol. Microbiol 13 (1994) 219-228
97. Saloheimo M., Nakari-Setälä T., Tenkanen M., and Penttilä M. cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast. Eur J Biochem 429 (1997) 584-591
98. 2 transcription factor involved in regulation ofactivity of the cellulase promoter cbhl of Trichoderma reesei. J Biol Chem 275 (2000) 5817-5825
99. Saul D.J., Williams L.C., Toogood H.S., Daniel R., and Bergquist P.L. Sequence of the gene encoding a highly thermostable neutral proteinase from Bacillus sp Strain EA expression in Eschericia coli and characterization. Biochim Biophys Acta 1308 (1996) 74-80
100. Strauss J., Mach R.L., Zeilinger S., Hartler G., Stoffler G., Wolschek M., and Kubicek C.P. Crel, the carbon catabolite repressor protein from Trichoderma reesei. FEBS Lett 376 (1995) 103-107
101. Suominen P., Mäntylä A., Karhunen T., and Nevalainen H. High frequency one-step gene replacement in Trichoderma reeseiII. Effects of deletion of individual cellulase genes. Mol Gen Genet 241 (1993) 522-530
102. Takashima S., Nakamura A., Iikura H., Masaki H., and Uozumi T. Cloning of a gene encoding a putative carbon catabolite repressor from Trichoderma reesei. Biosci Biotechnol Biochem 60 (1996) 173-176
103. Takashima S., Nakamura A., Hidaka M., Masaki H., and Uozumi T. Isolation of the creA gene from the cellulolytic fungus Humicola grisea and analysis of CreA binding sites upstream from the cellulase genes. Biosci Biotechnol Biochem 62 (1998) 2364-2370
104. Takegawa K., Kondo A., Iwamoto H., Fujiwara K., Hosokawa Y., Kato I., Hiromi K., and Iwahara S. Novel oligomannose-type sugar chains derived from glucose oxidase of Aspergillus niger. Biochem Int 25 (1991) 181-190
105. Tani S., Kawaguchi T., Kato M., Kobayashi T., and Tsukagoshi N. A novel nuclear factor, SREB, binds to a cis-acting element, SRE, required for inducible expression of the Aspergillus oryzae Taka-amylase A gene in A. nidulans. Mol Gen Genet 263 (2000) 232-238
106. Te'o V.J.S., Cziferszky A.E., Bergqulst P.L., and Nevalainen K.M.H. Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilumfor expression in the filamentous fungus Trichoderma reesei. FEMS Microbiol Lett 190 (2000) 13-19
107. Tilburn J., SarkarS W.D., Espeso E.A., Orejas M., Mungroo J., Penalva M.A., and Arst Jr. H.N. The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid-and alkaline-expressed genes by ambient pH. Embo J 14 (1995) 779-790
108. Turunen O., Vuorio M., Fenel F., and Leisola M. Engineering of multiple arginines into the Ser/Thr surface of Trichoderma reesei endo-l,4-beta-xylanase II increasesthe thermotolerance and shifts the pH optimum towards alkaline pH. Protein Eng 15 (2002) 141-145
109. Submitted. Valkonen M., Penttilä M., and Saloheimo M. The effect of inactivation and consitutive expression of the unfolded protein response pathway on protein production in Saccharomyces cerevisiae (2002)
110. van d.H.J., Jarai G., Buxton F.P., and Visser J. Cloning, characterization and expression of pepF, a gene encoding a serine carboxypeptidase from Aspergillus niger. Gene 151 (1994) 73-79
111. van d.H.J., van d.V.P., Fraissinet-Tachet L., and Visser J. Aspergillus as a host for heterologous protein production: the problem of proteases. TIBTECH 15 (1997) 256-263
112. van d.H.J., Fraissinet-Tachet L., van d.V.P., and Visser J. Production of the homologous pectin lyase B protein in six genetically defined protease-deficient Aspergillus niger mutant strains. Curr Genet 32 (1997) 73-81
113. van P.N., Gielkens M.M., de V.R., Visser J., and de G.L. The transcriptional activator XlnR regulates both xylanolytic and endoglucanase gene expression in Aspergillus niger. Appl Environ Microbiol 64 (1998) 3615-3619
114. van P., NN V.J., and de G.L. Isolation and analysis of xlnR, encoding a transcriptional activator co-ordinating xylanolytic expression in Aspergillus niger. Mol Microbiol 27 (1998) 131-142
115. Suppl. van Z.C., Punt P., Emalfarb M., Burlinghame R., Sinitsyn A., Parriche M., Bousson J.C., and van d.H.C. Chrysosporium lucknowense, a new fungal host for protein production. Fungal Genetics Newsletter 48 (1901) p 89
116. Vishniac H.S. Biodiversity of yeasts and filamentous fungi in terrestrial Antarctic ecosystems. Biodiversity and Conservation 5 (1996) 1365-1378
117. Ward M. Heterologous gene expression in Aspergillus. In: Nevalainen H., and Penttilä M. (Eds). Molecular Biology of Filamentous Fungi, Proceedings of the EMBO-Alko Workshop, Espoo Finland. Foundation for Biotechnical and Industrial Fermentation Research Vol. 6 (1989) 119-128
118. Ward M., Wilson L.L., Kodama K.H., Rey M.W., and Berka R.M. Improved production of chymosin in Aspergillus by expression as a glucoamylase-chymosin fusion. Bio/Technology 8 (1990) 435-440
119. Welihinda A.A., Tirasophon W., and Kaufman R.J. The cellular response to prtein misfolding in the endoplasmic reticulum. Gene Express 7 (1999) 293-300
120. Wendland J., Ayad-Durieux Y., Knechtle P., Rebischung C., and Philippsen P. PCR-based gene targeting in the filamentous fungus Ashbya gossypii. Gene 242 (2000) 381-391
121. Wickens M., Anderson P., and Jackson R.J. Life and death in the cytoplasm: messages from the. 3' end. 7 (1999) 220-232 Curr Opin Genet Dev
122. Xu J., Wang L., Ridgway D., Gu T., and Murray M.-Y. Increased heterologous protein production in Aspergillus niger fermentation through extracellular proteases inhibition by pelleted growth. Biotechnol Prog 16 (2000) 222-227
123. Zhao H., and Arnold F.H. Directed evolution converts subtilisin E into a functional equivalent of a thermitase. Protein Eng 12 (1999) 47-53