Assessment of bluetongue viraemia in sheep by real-time PCR and correlation with viral infectivity

Journal of Virological Methods

Volumn 169, Issue 2, 2010, Pages 305-315

  Chatzinasiou, E ^a   Dovas, C I ^a   Papanastassopoulou, M ^a   Georgiadis, M ^a   Psychas, V ^a   Bouzalas, I ^a   Koumbati, M ^a   Koptopoulos, G ^a   Papadopoulos, O ^a  

^a ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

Author keywords

Bluetongue virus; DsRNA denaturation; DsRNA viruses; EHDV amplification control; QRT PCR; RNA extraction

Indexed keywords

COMPLEMENTARY DNA; DOUBLE STRANDED RNA; VIRUS RNA;

ACCURACY; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD SAMPLING; BLUETONGUE; BLUETONGUE ORBIVIRUS; BLUETONGUE ORBIVIRUS 1; BLUETONGUE ORBIVIRUS 9; CONTROLLED STUDY; DENATURATION; DNA SYNTHESIS; EXPERIMENTAL INFECTION; FEMALE; GENE AMPLIFICATION; NONHUMAN; ORBIVIRUS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA PURIFICATION; SHEEP; VIREMIA; VIRUS GENOME; VIRUS INFECTIVITY; VIRUS TITRATION;

AFRICAN HORSE SICKNESS VIRUS; ANIMALS; BLUETONGUE; BLUETONGUE VIRUS; CHICK EMBRYO; FEMALE; HEMORRHAGIC DISEASE VIRUS, EPIZOOTIC; NUCLEIC ACID DENATURATION; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SHEEP; VIRAL LOAD; VIREMIA;

AFRICAN HORSESICKNESS VIRUS; BLUETONGUE VIRUS; DSRNA VIRUSES; EPIZOOTIC HEMORRHAGIC DISEASE VIRUS; OVIS ARIES;

EID: 77957018685     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.07.033     Document Type: Article

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