Rapid detection and quantitation of Bluetongue virus (BTV) using a Molecular Beacon fluorescent probe assay

Journal of Virological Methods

Volumn 137, Issue 1, 2006, Pages 34-42

  Orrù, Germano ^a   Ferrando, Maria Laura ^a   Meloni, Mauro ^a   Liciardi, Manuele ^c   Savini, Giovanni ^d   De Santis, Paola ^b  

^a UNIVERSITY OF CAGLIARI   (Italy)

^b ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLE REGIONI LAZIO E TOSCANA   (Italy)

^c ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLA SARDEGNA   (Italy)

^d ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELL ABRUZZO E DEL MOLISE G CAPORALE   (Italy)

Author keywords

Bluetongue virus; Molecular Beacon fluorescent probe; NS3 protein; Real time RT PCR

Indexed keywords

BLOOD EXTRACT; COMPLEMENTARY DNA; FLUORESCENT DYE;

ANIMAL CELL; ARTICLE; BLUETONGUE ORBIVIRUS; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; FLUORESCENCE ANALYSIS; LABORATORY DIAGNOSIS; LIVESTOCK; MOLECULAR BEACON; NONHUMAN; PRIORITY JOURNAL; PROTEIN TARGETING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA VIRUS; SAMPLE; SENSITIVITY AND SPECIFICITY; SEROTYPE; VIREMIA; VIRUS DETECTION; VIRUS INFECTION; VIRUS ISOLATION;

ANIMALS; BLOOD; BLUETONGUE VIRUS; DNA PRIMERS; FLUORESCENCE; FLUORESCENT DYES; MOLECULAR PROBE TECHNIQUES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL NONSTRUCTURAL PROTEINS;

ANIMALIA; BLUETONGUE VIRUS;

EID: 33747031436     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.05.028     Document Type: Article

References (52)

1. Afshar A. Bluetongue: laboratory diagnosis. Comp. Immunol. Microbiol. Infect. Dis. 17 (1994) 221-242
2. Akita G.Y., Chinsangaram J., Osburn B.I., Ianconescu M., and Kaufman R. Detection of Bluetongue virus serogroup by polymerase chain reaction. J. Vet. Diagn. Invest. 4 (1992) 400-405
3. Aradaib I.E., Schore C.E., Cullor J.S., and Osburn B.I. A nested PCR for detection of North American isolates of Bluetongue virus based on NS1 genome sequence analysis of BTV-17. Vet. Microbiol. 59 (1998) 99-108
4. Aradaib I.E., Smith W.L., Osburn B.I., and Cullor J.S. A multiplex PCR for simultaneous detection and differentiation of North American serotypes of Bluetongue and epizootic hemorrhagic disease viruses. Comp. Immunol. Microbiol. Infect. Dis. 26 (2003) 77-87
5. Billinis C., Koumbati M., Spyrou V., Nomikou K., Mangana O., Panagiotidis A., and Papadopoulos O. Bluetongue virus diagnosis of clinical cases by a duplex reverse transcription-PCR: a comparison with conventional methods. J. Virol. Methods 98 (2001) 77-89
6. Blacksell S.D., and Lunt R.A. A simplified fluorescence inhibition test for the serotype determination of Australian Bluetongue viruses. Aust. Vet. J. 73 (1996) 33-34
7. Bonneau K.R., DeMaula C.D., Mullens B.A., and MacLachlan N.J. Duration of viraemia infectious to Culicoides sonorensis in Bluetongue virus-infected cattle and sheep. Vet. Microbiol. 88 (2002) 115-125
8. Bonneau K.R., Mullens B.A., and Maclachlan N.J. Occurrence of genetic drift and founder effect during quasispecies evolution of the VP2 and NS3/NS3A genes of Bluetongue virus upon passage between sheep, cattle, and Culicoides sonorensis. J. Virol. 75 (2001) 8298-8305
9. Bonneau K.R., Zhang N., Zhu J., Zhang K., Xiao L., Xiang W., and MacLachlan N.J. Sequence comparison of the L2 and S10 genes of Bluetongue viruses from the United States and the People's Republic of China. Virus Res. 61 (1999) 153-160
10. Brown C.C., Myer R.F., and Grubman M.J. Use of a digoxigenin-labeled RNA probe to detect all 24 serotypes of Bluetongue virus in cell culture. J. Vet. Diagn. Invest. 5 (1993) 159-162
11. Bowne J.G., and Jones R.H. Observations on Bluetongue virus in the salivary glands of an insect vector, Culicoides variipennis. Virology 30 (1966) 127-133
12. Caporale V., Giovannini A., Patta C., Calistri P., Nannini D., and Santucci U. Vaccination in the control strategy of Bluetongue in Italy. Dev. Biol. (Basel) 119 (2004) 113-127
13. Chomczynski P., and Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162 1 (1987) 156-159
14. Clavijo A., Heckertm R.A., Dulac G.C., and Afshar A. Isolation and identification of Bluetongue virus. J. Virol. Methods 87 (2000) 13-23
15. Conte A., Colangeli P., Ippoliti C., Paladini C., Ambrosini M., Savini L., Dall'Acqua F., and Calistri P. The use of a web-based interactive geographical information system for the surveillance of Bluetongue in Italy. Rev. Sci. Tech. 24 3 (2005) 857-868
16. Davies F.G., Mungai J.N., and Pini A. A new Bluetongue virus serotype isolated in Kenya. Vet. Microbiol. 31 (1992) 25-32
17. Du Toit R.M. The transmission of Bluetongue and horse-sickness by Culicoides. Onderstepoort J. Vet. Sci. Anim. Ind. 19 (1944) 7-16
18. Erasmus B.J. Bluetongue in sheep and goats. Aust. Vet. J. 52 (1975) 196-198
19. Gorman B.M. The Bluetongue viruses. Curr. Top. Microbiol. Immunol. 162 (1990) 1-19
20. Han Z., and Harty R.N. The NS3 protein of Bluetongue virus exhibits viroporin-like properties. J. Biol. Chem. 279 (2004) 43092-43097
21. Hawkes R.A., Kirkland P.D., Sanders D.A., Zhang F., Li Z., Davis R.J., and Zhang N. Laboratory and field studies of an antigen capture ELISA for Bluetongue virus. J. Virol. Methods 85 (2000) 137-149
22. Howell P.G., Kumm N.A., and Botha M.J. The application of improved techniques to the identification of strains of Bluetongue virus. Onderstepoort J. Vet. Res. 37 (1970) 59-66
23. Howell P.G., and Verwoerd D.W. Bluetongue virus. In: Gard S., Hallaver C., and Meyer K.F. (Eds). Virology Monographs vol. 9 (1971), Springer Verlag, New York 35-74
24. Hwang G.Y., Yang Y.Y., Chiou J.F., and Li J.K. Sequence conservation among the cognate non-structural NS3:3A protein genes of six Bluetongue viruses. Virus Res. 23 (1992) 151-161
25. Jiménez-Clavero M.A., Agüero M., San Miguel E., Mayoral T., Cruz López M., Ruano M.J., Romero E., Monaco F., Polci A., Savini G., and Gómez-Tejedor C. High throughput detection of Bluetongue virus by a new real-time fluorogenic reverse transcription-polymerase chain reaction: application on clinical samples from current Mediterranean outbreaks. J. Vet. Diagn. Invest. 18 (2006) 7-17
26. Johnson D.J., Wilson W.C., and Paul P.S. Validation of a reverse transcriptase multiplex PCR test for the serotype determination of U.S. isolates of Bluetongue virus. Vet. Microbiol. 76 (2000) 105-115
27. Katz J.B., Alstad A.D., Gustafson G.A., and Moser K.M. Sensitive identification of Bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid. J. Clin. Microbiol. 31 (1993) 3028-3030
28. Kostrikis L.G., Tyagi S., Mhlanga M.M., Ho D.D., and Russell F.K. Molecular beacons: spectral genotyping of human alleles. Science 279 (1998) 1228-1229
29. Koumbati M., Mangana O., Nomikou K., Mellor P.S., and Papadopoulos O. Duration of Bluetongue viraemia and serological responses in experimentally infected European breeds of sheep and goats. Vet. Microbiol. 12 (1999) 277-285
30. Lanciotti R.S., Kerst A.J., Nasci R.S., et al. Rapid detection of West Nile from human clinical specimens, field collection mosquitoes, and avian samples by a TaqMan riverse-transcriptase-PCR assay. J. Clin. Microbiol. 38 (2000) 4066-4071
31. Lanciotti R.S., and Kerst A.J. Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses. J. Clin. Microbiol. 39 (2001) 4506-4513
32. Landry M.L., Garner R., and Ferguson D. Real-time nucleic acid sequence-based amplification using Molecular Beacons for detection of enterovirus RNA in clinical specimens. J. Clin. Microbiol. 43 (2005) 3136-3139
33. MacLachlan N.J., Nunamaker R.A., Katz J.B., Sawyer M.M., Akita G.Y., Osburn B.I., and Tabachnick W.J. Detection of Bluetongue virus in the blood of inoculated calves: comparison of virus isolation, PCR assay, and in vitro feeding of Culicoides variipennis. Arch. Virol. 136 (1994) 1-8
34. Marras S.A., Kramer F.R., and Tyagi S. Multiplex detection of single-nucleotide variations using Molecular Beacons. Genet. Anal. 14 (1999) 151-156
35. McColl K.A., and Gould A.R. Detection and characterisation of Bluetongue virus using the polymerase chain reaction. Virus Res. 21 (1991) 19-34
36. Mecham J.O. Detection of Bluetongue virus from blood of infected sheep by use of an antigen-capture enzyme-linked immunosorbent assay after amplification of the virus in cell culture. Am. J. Vet. Res. 54 (1993) 370-372
37. Mellor P.S., and Wittmann E.J. Bluetongue virus in the Mediterranean Basin 1998-2001. Vet. J. 164 (2002) 20-37
38. Mertens P.P., and Diprose J. The Bluetongue virus core: a nano-scale transcription machine. Virus Res. 101 (2004) 29-43
39. Orrù G., De Santis P., Solinas F., Savini G., Piras V., and Caporale V. Differentiation of Italian field and South African vaccine strains of Bluetongue virus serotype 2 using real-time PCR. J. Virol. Methods 122 (2004) 37-43
40. Pearson J.E., Gustafson A.L., Shafer A.L., and Alstad A.D. Diagnosis of Bluetongue and epizootic hemorrhagic disease. In: Walton T.E., and Osburn B.I. (Eds). Bluetongue, African Horse Sickness, and Related Orbiviruses. Proceedings of the Second International Symposium. Boca Raton (1991), CRC Press 533-546
41. Pierce C.M., Balasuriya U.B., and MacLachlan N.J. Phylogenetic analysis of the S10 gene of field and laboratory strains of Bluetongue virus from the United States. Virus Res. 55 (1998) 15-27
42. Price D.A., and Hardy W.T. Isolation of the Bluetongue virus from Texas sheep-Culicoides shown to be a vector. J. Am. Vet. Med. Assoc. 124 (1954) 255-258
43. Poddar S.K. Detection of adenovirus using PCR and Molecular Beacon. J. Virol. Methods 82 (1989) 19-26
44. Roy P. Bluetongue virus genetics and genome structure. Virus Res. 13 (1989) 179-206
45. Roy P., Marshall J.J.A., and French T.J. Structure of Bluetongue virus genome and its encoded proteins. In: Roy P., and Gorman B.M. (Eds). Current Topics in Microbiology and Immunology (1990), Springer, Berlin
46. Roy P. Bluetongue virus proteins. J. Gen. Virol. 73 (1992) 3051-3064
47. Singer R.S., Boyce W.M., Gardner I.A., Johnson W.O., and Fisher A.S. Evaluation of Bluetongue virus diagnostic test in free-ranging bighorn sheep. Prev. Vet. Med. 35 (1998) 265-282
48. Stanislawek W.L., Lunt R.A., Blacksell S.D., Newberry K.M., Hooper P.T., and White J.R. Detection by ELISA of Bluetongue antigen directly in the blood of experimentally infected sheep. Vet. Microbiol. 52 (1996) 1-12
49. Tan W., Fang X., Li J., and Liu X. Molecular Beacons: a novel DNA probe for nucleic acid and protein studies. Chemistry 6 (2000) 1107-1111
50. Tyagi S., Bratu D.P., and Kramer F.R. Multicolor Molecular Beacons for allele discrimination. Nat. Biotechnol. 16 (1998) 49-53
51. Wade-Evans A.M. Complete sequence of genome segment 10, encoding the NS3 protein, of Bluetongue virus, serotype 1 from South Africa. Nucleic Acids Res. 18 (1990) 4920
52. Zhou E.M., Ridd D., Riva J., Fernando L., and Clavijo A. Development and evaluation of an IgM-capture ELISA for detection of recent infection with Bluetongue viruses in cattle. J. Virol. Methods 91 (2001) 175-182