Novel sulfoxides facilitate GC-rich template amplification

BioTechniques

Volumn 32, Issue 4, 2002, Pages 866-874

  Chakrabarti, Raj ^a   Schutt, Clarence E ^a  

^a PRINCETON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLIFICATION; ORGANIC SOLVENTS; SULFUR COMPOUNDS;

ENHANCERS;

BIOTECHNOLOGY;

AMIDE; BETAINE; CYCLOBUTANE DERIVATIVE; DIMETHYL SULFOXIDE; PRIMER DNA; SULFONE DERIVATIVE; SULFOXIDE;

ARTICLE; DNA TEMPLATE; ENHANCER REGION; GENE AMPLIFICATION; POLYMERASE CHAIN REACTION; STRUCTURE ACTIVITY RELATION;

EID: 0036216184     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02324rr04     Document Type: Article

References (17)

1. Improvement of PCR amplified DNA sequencing with the aid of detergents
2. Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content
3. The enhancement of PCR amplification by low-molecular weight amides
4. The enhancement of PCR amplification by low molecular-weight sulfones
5. Effective amplification of long targets from cloned inserts and human genomic DNA
6. PCR in genetic diagnosis
7. Unwinding of double-stranded DNA helix by dehydration
8. Localized sequence regions possessing high melting temperatures prevent the amplification of a DNA mimic in competitive PCR
9. Organic solvents as facilitators of polymerase chain reaction
10. Optimization and troubleshooting in PCR
11. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
12. Using cosolvents to enhance PCR amplification
13. Denaturants or cosolvents improve the specificity of PCR amplification of a G+C rich DNA using genetically engineered DNA polymerases
14. Strategy for controlling preferential amplification and avoiding false negatives in PCR typing reactions
15. An improved method for directly sequencing PCR amplified material using dimethylsulfoxide