A highly efficient method for long-chain cDNA synthesis using trehalose and betaine

Analytical Biochemistry

Volumn 301, Issue 2, 2002, Pages 168-174

  Spiess, Andrej Nikolai ^a   Ivell, Richard ^a  

^a Institut fur Hormon und Fortpflanzungsforschung IHF   (Germany)

Author keywords

cDNA synthesis; Melting temperature; Secondary structure; Thermostabilization

Indexed keywords

MELTING POINT; MOLECULAR BIOLOGY; POLYMERASE CHAIN REACTION; TRANSCRIPTION;

CDNA LIBRARIES; CDNA SYNTHESIS; FULL-LENGTH CDNA; LABORATORY METHODS; RAPID AMPLIFICATION OF CDNA ENDS; REVERSE TRANSCRIPTION; SECONDARY STRUCTURES; THERMOSTABILIZATION;

BETAINES;

BETAINE; CLATHRIN; COMPLEMENTARY DNA; MESSENGER RNA; TREHALOSE;

ANALYTIC METHOD; ARTICLE; COMBINATORIAL CHEMISTRY; CONTROLLED STUDY; DIAGNOSTIC VALUE; DNA LIBRARY; DNA STRUCTURE; DNA SYNTHESIS; GENE AMPLIFICATION; GENE CONSTRUCT; MELTING POINT; MOLECULAR BIOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; TEMPERATURE DEPENDENCE;

EID: 0037083546     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5474     Document Type: Article

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