Identification of Cryptosporidium species and genotypes in Scottish raw and drinking waters during a one-year monitoring period

Applied and Environmental Microbiology

Volumn 76, Issue 17, 2010, Pages 5977-5986

  Nichols, R A B ^a   Connelly, L ^a   Sullivan, C B ^a   Smith, H V ^a  

^a STOBHILL HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

18S RRNA; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; CRYPTOSPORIDIUM SPP; DRINKING WATER; MONITORING PERIODS; NESTED PCR; OOCYSTS; PCR ASSAY; RAW WATER; RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; SPECIES IDENTIFICATION;

AGRICULTURAL PRODUCTS; ASSAYS; RNA;

POTABLE WATER;

PROTOZOAL DNA; RIBOSOME DNA; RNA 18S; WATER;

DRINKING WATER; GENOTYPE; HEALTH RISK; IDENTIFICATION METHOD; MONITORING; POLYMERASE CHAIN REACTION; PROTOZOAN; RNA; WATER QUALITY;

ARTICLE; CHEMISTRY; CLASSIFICATION; CRYPTOSPORIDIUM; CYTOLOGY; DNA FINGERPRINTING; DNA SEQUENCE; GENETICS; GENOTYPE; HUMAN; ISOLATION AND PURIFICATION; MICROSCOPY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PARASITOLOGY; PHYSICAL ANTHROPOLOGY; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; UNITED KINGDOM;

CRYPTOSPORIDIUM; DNA FINGERPRINTING; DNA, PROTOZOAN; DNA, RIBOSOMAL; FORENSIC ANTHROPOLOGY; GENOTYPE; HUMANS; MICROSCOPY; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RNA, RIBOSOMAL, 18S; SCOTLAND; SEQUENCE ANALYSIS, DNA; WATER;

CERVIDAE; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM ANDERSONI; CRYPTOSPORIDIUM PARVUM; CRYPTOSPORIDIUM SP.;

EID: 77956591867     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00915-10     Document Type: Article

References (30)

1. Cacciò, S. M., R. C. A. Thompson, J. McLauchlin, and H. V. Smith. 2005. Unravelling Cryptosporidium and Giardia epidemiology. Trends Parasitol. 21:430-437.
2. da Silva, A. J., S. Caccio, C. Williams, K. Y. Won, E. K. Nace, C. Whittier, N. J. Pieniazek, and M. L. Eberhard. 2003. Molecular and morphologic characterization of a Cryptosporidium genotype identified in lemurs. Vet. Parasitol. 111:297-307.
3. Fayer, R., U. M. Morgan, and S. J. Upton. 2000. Epidemiology of Cryptosporidium: transmission, detection and identification. Int. J. Parasitol. 30:1305-1322.
4. Fayer, R., M. Santin, and D. Macarisin. 2010. Cryptosporidium ubiquitum n. sp. in animals and humans. Vet. Parasitol. doi:10.1016/j.vetpar.2010.04.028.
5. Feng, Y., Y. Ortega, G. He, P. Das, M. Xu, X. Zhang, R. Fayer, W. Gatei, V. Cama, and L. Xiao. 2007. Wide geographic distribution of Cryptosporidium bovis and the deer-like genotype in bovines. Vet. Parasitol. 144:1-9.
6. Girdwood, R. W. A., and H. V. Smith. 1999. Cryptosporidium, p. 487-497. In R. Robinson, C. Batt, and P. Patel (ed.), Encyclopaedia of food microbiology. Academic Press, London, United Kingdom.
7. Jiang, J., K. A. Alderisio, and L. Xiao. 2005. Distribution of Cryptosporidium genotypes in storm event water samples from three watersheds in New York. Appl. Environ. Microbiol. 71:4446-4454.
8. Johnson, D. W., N. J. Pieniazek, D. W. Griffin, L. Misener, and J. B. Rose. 1995. Development of a PCR protocol for sensitive detection of Cryptosporidium in water samples. Appl. Environ. Microbiol. 61:3849-3855.
9. Kváč, M., B. S. Kvetonova, and O. Ditrich. 2009. Cryptosporidium pig genotype II in immunocompetent man. Emerg. Infect. Dis. 15:982-983.
10. Nichols, R. A. B., and H. V. Smith. 2004. Optimisation of DNA extraction and molecular detection of Cryptosporidium oocysts in natural mineral water sources. J. Food Prot. 67:524-532.
11. Nichols, R. A. B., B. M. Campbell, and H. V. Smith. 2003. Identification of Cryptosporidium spp. oocysts in United Kingdom noncarbonated natural mineral waters and drinking waters by using a modified nested PCR-restriction fragment length polymorphism assay. Appl. Environ. Microbiol. 69:4183-4189.
12. Nichols, R. A. B., B. M. Campbell, and H. V. Smith. 2006. Molecular fingerprinting of Cryptosporidium species oocysts isolated during water monitoring. Appl. Environ. Microbiol. 72:5428-5435.
13. Plutzer, J., and P. Karanis. 2009. Genetic polymorphism in Cryptosporidium: an update. Vet. Parasitol. 165:187-199.
14. Ruecker, N. J., N. Bounsombath, P. Wallis, C. S. L. Ong, J. L. Isaac-Renton, and N. F. Neumann. 2005. Molecular forensic profiling of Cryptosporidium species and genotypes in raw water. Appl. Environ. Microbiol. 71:8991-8994.
15. Ruecker, N. J., S. L. Braithwaite, E. Topp, T. Edge, D. R. Lapen, G. Wilkes, W. Robertson, D. Medeiros, C. W. Sensen, and N. F. Neumann. 2007. Tracking host sources of Cryptosporidium spp. in raw water for improved health risk assessment. Appl. Environ. Microbiol. 73:3945-3957.
16. Slifko, T. R., H. V. Smith, and J. B. Rose. 2000. Emerging parasite zoonoses associated with water and food. Int. J. Parasitol. 30:1379-1393.
17. Smith, H. V., and J. B. Rose. 1990. Waterborne cryptosporidiosis. Parasitol. Today 6:8-12.
18. Smith, H. V., and J. B. Rose. 1998. Waterborne cryptosporidiosis: current status. Parasitol. Today 14:14-22.
19. Smith, H. V., and R. A. B. Nichols. 2009. Preparation of parasitic specimens for direct molecular applications, p. 317-380. In D. Liu (ed.), Handbook of nucleic acid purification. CRC Press, Boca Raton, FL.
20. Smith, H. V. and, R. A. B. Nichols. 2010. Cryptosporidium: detection in water and food. Exp. Parasitol. 124:61-79.
21. Smith, H. V., L. J. Robertson, and J. E. Ongerth. 1995. Cryptosporidiosis and giardiasis: the impact of waterborne transmission. J. Water SRT Aqua 44:258-274.
22. Smith, H. V., S. M. Cacciò, A. Tait, J. McLauchlin, and R. C. A. Thompson. 2006. Tools for investigating the abiotic transmission of Cryptosporidium and Giardia infections in humans. Trends Parasitol. 22:160-166.
23. Taberlet, P., S. Griffin, B. Goossens, S. Questiau, V. Manceau, N. Escaravage, L. P. Waits, and J. Bouvet. 1996. Reliable genotyping of samples with very low DNA quantities using PCR. Nucleic Acids Res. 24:3189-3194.
24. Tamura, K., J. Dudley, M. Neil, and S. Kumar. 2007. MEGA4: molecular evolutionary analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599.
25. Ward, P. I., P. Deplazes, W. Regli, H. Rinder, and A. Mathis. 2002. Detection of eight Cryptosporidium genotypes in surface and waste waters in Europe. Parasitology 124:359-368.
26. Xiao, L., U. M. Morgan, J. Limor, L. Escalante, M. Arrowood, W. Shulaw, R. C. A. Thompson, R. Fayer, and A. A. Lal. 1999. Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Appl. Environ. Microbiol. 65:3386-3391.
27. Xiao, L., K. Alderisio, J. Limor, M. Royer, and A. A. Lal. 2000. Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool. Appl. Environ. Microbiol. 66:5492-5498.
28. Xiao, L., A. Singh, J. Limor, T. K. Graczyk, S. Gradus, and A. A. Lal. 2001. Molecular characterization of Cryptosporidium oocysts in samples of raw surface water and wastewater. Appl. Environ. Microbiol. 67:1097-1101.
29. Xiao, L., and U. M. Ryan. 2008. Molecular epidemiology, p. 119-172. In R. Fayer and L. Xiao (ed.), Cryptosporidium and cryptosporidiosis, 2nd ed. CRC Press, Boca Raton, FL.
30. Yang, W., P. Chen, E. N. Villegas, R. B. Landy, C. Kanetsky, V. Cama, T. Dearen, C. L. Schultz, K. G. Orndorff, G. J. Prelewicz, M. H. Brown, K. R. Young, and L. Xiao. 2008. Cryptosporidium source tracking in the Potomac River watershed. Appl. Environ. Microbiol. 74:6495-6504.