Eugenol reduces the expression of virulence-related exoproteins in Staphylococcus aureus

Applied and Environmental Microbiology

Volumn 76, Issue 17, 2010, Pages 5846-5851

  Qiu, Jiazhang ^a   Feng, Haihua ^a   Lu, Jing ^a   Xiang, Hua ^a   Wang, Dacheng ^a   Dong, Jing ^a   Wang, Jianfeng ^a   Wang, Xiaoliang ^a   Liu, Juxiong ^a   Deng, Xuming ^a  

^a JILIN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ANTI-MICROBIAL AGENT; ESSENTIAL OIL COMPONENTS; GRAM-NEGATIVE BACTERIA; GROWTH OF BACTERIA; HEMOLYTIC ACTIVITY; NOVEL FOODS; PCR ANALYSIS; REVERSE TRANSCRIPTION; S. AUREUS; STAPHYLOCOCCAL ENTEROTOXIN A; STAPHYLOCOCCUS AUREUS; SUPERNATANTS; TOXIC SHOCKS; TRANSCRIPTIONAL ANALYSIS; TRANSCRIPTIONAL LEVELS; TUMOR NECROSIS FACTORS; WESTERN-BLOT ANALYSIS;

BACTERIA; BACTERIOLOGY; BLOOD; ESSENTIAL OILS; FOOD PRODUCTS;

TRANSCRIPTION;

ANTIINFECTIVE AGENT; BACTERIAL TOXIN; EUGENOL; TUMOR NECROSIS FACTOR ALPHA; VIRULENCE FACTOR;

ANTIMICROBIAL ACTIVITY; BACTERIUM; BIOASSAY; ESSENTIAL OIL; POLYMERASE CHAIN REACTION; PROTEIN; TOXIN; TUMOR; VIRULENCE;

ARTICLE; BIOSYNTHESIS; DRUG EFFECT; GENE EXPRESSION; GENE EXPRESSION PROFILING; HEMOLYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SECRETION; STAPHYLOCOCCUS AUREUS; VIRULENCE;

ANTI-BACTERIAL AGENTS; BACTERIAL TOXINS; EUGENOL; GENE EXPRESSION; GENE EXPRESSION PROFILING; HEMOLYSIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS; TUMOR NECROSIS FACTOR-ALPHA; VIRULENCE; VIRULENCE FACTORS;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; POSIBACTERIA; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 77956590595     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00704-10     Document Type: Article

References (38)

1. Applied Biosystems. 1997. Relative quantification of gene expression. User bulletin no. 2. Applied Biosystems, Courtaboeuf, France.
2. 1a. Arvidson, S., and K. Tegmark. 2001. Regulation of virulence determinants in Staphylococcus aureus. Int. J. Food Microbiol. 291:159-170.
3. Astani, A., J. Reichling, and P. Schnitzler. 15 December 2009, posting date. Screening for antiviral activities of isolated compounds from essential oils. Evidence-based complement. Alternat. Med. doi:10.1093/ecam/nep187.
4. Balaban, N., and A. Rasooly. 2000. Staphylococcal enterotoxins. Int. J. Food Microbiol. 61:1-10.
5. Ben Arfa, A., S. Combes, L. Preziosi-Belloy, N. Gontard, and P. Chalier. 2006. Antimicrobial activity of carvacrol related to its chemical structure. Lett. Appl. Microbiol. 43:149-154.
6. Bernardo, K., N. Pakulat, S. Fleer, A. Schnaith, O. Utermohlen, and O. Krut. 2004. Subinhibitory concentrations of linezolid reduce Staphylococcus aureus virulence factor expression. Antimicrob. Agents Chemother. 48:546-555.
7. Blaszyk, M., and R. A. Holley. 1998. Interaction of monolaurin, eugenol and sodium citrate on growth of common meat spoilage and pathogenic organisms. Int. J. Food Microbiol. 39:175-183.
8. Burt, S. 2004. Essential oils: their antibacterial properties and potential applications in foods-a review. Int. J. Food Microbiol. 94:223-253.
9. Cegelski, L., G. R. Marshall, G. R. Eldridge, and S. J. Hultgren. 2008. The biology and future prospects of antivirulence therapies. Nat. Rev. Microbiol. 6:17-27.
10. Chami, N., S. Bennis, F. Chami, A. Aboussekhra, and A. Remmal. 2005. Study of anticandidal activity of carvacrol and eugenol in vitro and in vivo. Oral Microbiol. Immunol. 20:106-111.
11. Chan, P. F., and S. J. Foster. 1998. Role of SarA in virulence determinant production and environmental signal transduction in Staphylococcus aureus. J. Bacteriol. 180:6232-6241.
12. Clinical and Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing; 15th informational supplement. CLSI/NCCLS document M100-S15. Clinical and Laboratory Standards Institute, Wayne, PA.
13. Dinges, M. M., P. M. Orwin, and P. M. Schlievert. 2000. Exotoxins of Staphylococcus aureus. Clin. Microbiol. Rev. 13:16-34.
14. Edwards-Jones, V., and H. A. Foster. 2002. Effects of silver sulphadiazine on the production of exoproteins by Staphylococcus aureus. J. Med. Microbiol. 51:50-55.
15. Gill, A. O., and R. A. Holley. 2004. Mechanisms of bactericidal action of cinnamaldehyde against Listeria monocytogenes and of eugenol against L. monocytogenes and Lactobacillus sakei. Appl. Environ. Microbiol. 70:5750-5755.
16. Giraudo, A. T., A. L. Cheung, and R. Nagel. 1997. The sae locus of Staphylococcus aureus controls exoprotein synthesis at the transcriptional level. Arch. Microbiol. 168:53-58.
17. Hashimoto, S., K. Uchiyama, M. Maeda, K. Ishitsuka, K. Furumoto, and Y. Nakamura. 1988. In vivo and in vitro effects of zinc oxide-eugenol (ZOE) on biosynthesis of cyclo-oxygenase products in rat dental pulp. J. Dent. Res. 67:1092-1096.
18. Herbert, S., P. Barry, and R. P. Novick. 2001. Subinhibitory clindamycin differentially inhibits transcription of exoprotein genes in Staphylococcus aureus. Infect. Immun. 69:2996-3003.
19. Jay, J. J., and G. M. Rivers. 2007. Antimicrobial activity of some food flavoring compounds. J. Food Saf. 6:129-139.
20. Juven, B. J., J. Kanner, F. Schved, and H. Weisslowicz. 1994. Factors that interact with the antibiotic action of thyme essential oil and its active constituents. J. Appl. Bacteriol. 76:626-631.
21. Koszczol, C., K. Bernardo, M. Kronke, and O. Krut. 2006. Subinhibitory quinupristin/dalfopristin attenuates virulence of Staphylococcus aureus. J. Antimicrob. Chemother. 58:564-574.
22. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature 227:680-685.
23. Le Loir, Y., F. Baron, and M. Gautier. 2003. Staphylococcus aureus and food poisoning. Genet. Mol. Res. 31:63-76.
24. Lin, Y. C., P. M. Schlievert, M. J. Anderson, C. L. Fair, M. M. Schaefers, R. Muthyala, and M. L. Peterson. 2009. Glycerol monolaurate and dodecylglycerol effects on Staphylococcus aureus and toxic shock syndrome toxin-1 in vitro and in vivo. PLoS One 9: e7499.
25. Lowy, F. D. 1998. Staphylococcus aureus infections. N. Engl. J. Med. 339:520-532.
26. Mohammed, M. J., and F. A. Al-Bayati. 2009. Isolation and identification of antibacterial compounds from Thymus kotschyanus aerial parts and Dianthus caryophyllus flower buds. Phytomedicine 16:632-637.
27. Moleyar, V., and P. Narasimham. 1992. Antibacterial activity of essential oil components. Int. J. Food Microbiol. 16:337-342.
28. Nagababu, E., and N. Lakshmaiah. 1997. Inhibition of xanthine oxidasexanthine-iron mediated lipid peroxidation by eugenol in liposomes. Mol. Cell. Biochem. 166:65-71.
29. Novick, R. P. 2003. Autoinduction and signal transduction in the regulation of staphylococcal virulence. Mol. Microbiol. 48:1429-1449.
30. Novick, R. P., H. F. Ross, S. J. Projan, J. Kornblum, B. Kreiswirth, and S. Moghazeh. 1993. Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule. EMBO J. 12:3967-3975.
31. Oscarsson, J., A. Kanth, K. Tegmark-Wisell, and S. Arvidson. 2006. SarA is a repressor of hla (α-hemolysin) transcription in Staphylococcus aureus: its apparent role as an activator of hla in the prototype strain NCTC 8325 depends on reduced expression of sarS. J. Bacteriol. 188:8526-8533.
32. Peng, H. L., R. P. Novick, B. Kreiswirth, J. Kornblum, and P. Schlievert. 1988. Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus. J. Bacteriol. 170:4365-4372.
33. Qiu, J., Y. Jiang, L. Xia, H. Xiang, H. Feng, S. Pu, N. Huang, L. Yu, and X. Deng. 2010. Subinhibitory concentrations of licochalcone A decrease alphatoxin production in both methicillin-sensitive and methicillin-resistant Staphylococcus aureus isolates. Lett. Appl. Microbiol. 50:223-229.
34. Rowe, G. E., and R. A. Welch. 1994. Assays of hemolytic toxins. Methods Enzymol. 235:657-667.
35. Sambanthamoorthy, K., M. S. Smeltzer, and M. O. Elasri. 2006. Identification and characterization of msa (SA1233), a gene involved in expression of SarA and several virulence factors in Staphylococcus aureus. Microbiology 152:2559-2572.
36. Smith-Palmer, A., J. Stewart, and L. Fyfe. 2004. Influence of subinhibitory concentrations of plant essential oils on the production of enterotoxins A and B and alpha-toxin by Staphylococcus aureus. J. Med. Microbiol. 53:1023-1027.
37. Tseng, C. W., and G. C. Stewart. 2005. Rot repression of enterotoxin B expression in Staphylococcus aureus. J. Bacteriol. 187:5301-5309.
38. Tsukiyama, R., H. Katsura, N. Tokuriki, and M. Kobayashi. 2002. Antibacterial activity of licochalcone A against spore-forming bacteria. Antimicrob. Agents Chemother. 46:1226-1230.