Subinhibitory concentrations of licochalcone A decrease alpha-toxin production in both methicillin-sensitive and methicillin-resistant Staphylococcus aureus isolates

Letters in Applied Microbiology

Volumn 50, Issue 2, 2010, Pages 223-229

  Qiu, J ^a   Jiang, Y ^b   Xia, L ^c   Xiang, H ^a   Feng, H ^a   Pu, S ^c   Huang, N ^a   Yu, L ^a   Deng, X ^a  

^a JILIN UNIVERSITY   (China)

^b Heilongjiang August First Agricultural University   (China)

^c YANGZHOU UNIVERSITY   (China)

Author keywords

Agr; Alpha toxin; Haemolysis; Licochalcone A; Staphylococcus aureus

Indexed keywords

BLOOD; GENE ENCODING; LEAD COMPOUNDS; TRANSCRIPTION;

ANTIBACTERIAL AGENT; CHALCONES; GENE REGULATORS; HAEMOLYSINS; HAEMOLYSIS; IMMUNOBLOT ANALYSIS; METHICILLIN; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MRNA LEVEL; QUANTITATIVE RT-PCR; STAPHYLOCOCCUS AUREUS; SUPERNATANTS; TOXIN PRODUCTION; TWO COMPONENT SYSTEMS;

TOXIC MATERIALS;

AGR PROTEIN; ALPHA TOXIN; BACTERIAL PROTEIN; HEMOLYSIN; HLA PROTEIN; LICOCHALCONE A; MESSENGER RNA; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; BACTERIUM; GROWTH RATE; INHIBITOR; POLYMERASE CHAIN REACTION; RNA; SECRETION;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM ISOLATE; CONTROLLED STUDY; DRUG EFFECT; GENETIC TRANSCRIPTION; HEMOLYSIS; IMMUNOBLOTTING; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; QUANTITATIVE ANALYSIS; REGULATOR GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS; SUPERNATANT;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS AUREUS;

EID: 74049118359     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2009.02783.x     Document Type: Article

References (35)

1. Applied Biosystems, User bulletin no. 2 (1997) Relative Quantification of Gene Expression. Courtaboeuf, France : Applied Biosystems.
2. Baltina, L.A. (2003) Chemical modification of glycyrrhizic acid as a route to new bioactive components for medicine. Curr Med Chem 10, 155 171.
3. Bernardo, K., Pakulat, N., Fleer, S., Schnaith, A., Utermöhlen, O., Krut, O., Müller, S. Krönke, M. (2004) Subinhibitory concentrations of linezolid reduce Staphylococcus aureus virulence factor expression. Antimicrob Agents Chemother 48, 546 555.
4. Bhakdi, S. Tranum-Jensen, J. (1991) Alpha-toxin of Staphylococcus aureus. Microbiol Rev 55, 733 751.
5. Chan, P.F. Foster, S.J. (1998) Role of SarA in virulence determinant production and environmental signal transduction in Staphylococcus aureus. J Bacteriol 180, 6232 6241.
6. Chen, M., Zhai, L., Christensen, S.B., Theander, T.G. Kharazmi, A. (2001) Inhibition of fumarate reductase in Leishmania major and L. donovani by chalcones. Antimicrob Agents Chemother 45, 2023 2029.
7. Clinical and Laboratory Standards Institute (2005) Performance Standards for Antimicrobial Susceptibility Testing; Fifteenth Informational Supplement. CLSI/NCCLS document M100-S15. Wayne, PA : Clinical and Laboratory Standards Institute (CLSI).
8. Fukai, T., Marumo, A., Kaitou, K., Kanda, T., Terada, S. Nomura, T. (2002) Antimicrobial activity of licorice flavonoids against methicillin-resistant Staphylococcus aureus. Fitoterapia 73, 536 539.
9. Giraudo, A.T., Cheung, A.L. Nagel, R. (1997) The sae locus of Staphylococcus aureus controls exoprotein synthesis at the transcriptional level. Arch Microbiol 168, 53 58.
10. Haraguchi, H., Ishikawa, H., Mizutani, K., Tamura, Y. Kinoshita, T. (1998) Antioxidative and superoxide scavenging activities of retrochalcones in Glycyrrhiza inflata. Bioorg Med Chem 6, 339 347.
11. Hatano, T., Shintani, Y., Aga, Y., Shiota, S., Tsuchiya, T. Yoshida, T. (2000) Phenolic constituents of licorice. VIII. Structures of glicophenone and glicoisoflavanone, and effects of licorice phenolics on methicillin-resistant Staphylococcus aureus. Chem Pharm Bull (Tokyo) 48, 1286 1292.
12. Herbert, S., Barry, P. Novick, R.P. (2001) Subinhibitory clindamycin differentially inhibits transcription of exoprotein genes in Staphylococcus aureus. Infect Immun 69, 2996 3003.
13. Ji, G., Beavis, R.C. Novick, R.P. (1995) Cell density control of staphylococcal virulence mediated by an octapeptide pheromone. Proc Natl Acad Sci USA 92, 12055 12059.
14. Kernodle, D.S., McGraw, P.A., Barg, N.L., Menzies, B.E., Voladri, R.K. Harshman, S. (1995) Growth of Staphylococcus aureus with nafcillin in vitro induces alpha-toxin production and increases the lethal activity of sterile broth filtrates in a murine model. J Infect Dis 172, 410 419.
15. Kuroda, H., Kuroda, M., Cui, L. Hiramatsu, K. (2007) Subinhibitory concentrations of beta-lactam induce haemolytic activity in Staphylococcus aureus through the SaeRS two-component system. FEMS Microbiol Lett 268, 98 105.
16. Kwon, H.S., Park, J.H., Kim, D.H., Kim, Y.H., Park, J.H., Shin, H.K. Kim, J.K. (2008) Licochalcone A isolated from licorice suppresses lipopolysaccharide-stimulated inflammatory reactions in RAW264.7 cells and endotoxin shock in mice. J Mol Med 86, 1287 1295.
17. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680 685.
18. Li, D. Cheung, A. (2008) Repression of hla by rot Is Dependent on sae in Staphylococcus aureus. Infect Immun 76, 1068 1075.
19. Liu, X., Xu, Y. Go, M. (2008) Functionalized chalcones with basic functionalities have antibacterial activity against drug sensitive Staphylococcus aureus. Eur J Med Chem 43, 1681 1687.
20. Nomura, T. Fukai, T. (1998) Phenolic constituents of licorice (Glycyrrhiza species). Fortschr Chem Org Naturst 73, 1 158.
21. Novick, R.P., Projan, S.J., Kornblum, J., Ross, H.F., Ji, G., Kreiswirth, B., Vandenesch, F. Moghazeh, S. (1995) The agr P2 operon: an autocatalytic sensory transduction system in Staphylococcus aureus. Mol Gen Genet 248, 446 458.
22. Ohlsen, K., Koller, K.-P. Hacker, J. (1997) Analysis of expression of the alpha-toxin gene (hla) of Staphylococcus aureus by using a chromosomally encoded hla::lacZ gene fusion. Infect Immun 65, 3606 3614.
23. Ohlsen, K., Ziebuhr, W., Koller, K.-P., Hell, W., Wichelhaus, T.A. Hacker, J. (1998) Effects of subinhibitory concentrations of antibiotics on alpha-toxin (hla) gene expression of methicillin-sensitive and methicillin-resistant Staphylococcus aureus isolates. Antimicrob Agents Chemother 42, 2817 2823.
24. Oscarsson, J., Kanth, A., Tegmark-Wisell, K. Arvidson, S. (2006) SarA is a repressor of hla (alpha-hemolysin) transcription in Staphylococcus aureus: its apparent role as an activator of hla in the prototype strain NCTC 8325 depends on reduced expression of sarS. J Bacteriol 188, 8526 8533.
25. Peng, H.L., Novick, R.P., Kreiswirth, B., Kornblum, J. Schlievert, P. (1988) Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus. J Bacteriol 170, 4365 4372.
26. Recsei, P., Kreiswirth, B., O'Reilly, M., Schlievert, P., Gruss, A. Novick, R.P. (1986) Regulation of exoprotein gene expression in Staphylococcus aureus by agr. Mol Gen Genet 202, 58 61.
27. Rowe, G.E. Welch, R.A. (1994) Assays of hemolytic toxins. Methods Enzymol 235, 657 667.
28. Saitoh, T. Shibata, S. (1975) New type of chalcones from licorice root. Tetrahedron Lett 16, 4461 4462.
29. Sambanthamoorthy, K., Smeltzer, M.S. Elasri, M.O. (2006) Identification and characterization of msa (SA1233), a gene involved in expression of SarA and several virulence factors in Staphylococcus aureus. Microbiology 152, 2559 2572.
30. Shah, S., Stapleton, P.D. Taylor, P.W. (2008) The polyphenol (-)-epicatechin gallate disrupts the secretion of virulence-related proteins by Staphylococcus aureus. Lett Appl Microbiol 46, 181 185.
31. Shibata, S. (1994) Anti-tumorigenic chalcones. Stem Cells 12, 44 52.
32. Shibata, S. (2000) A drug over the millennia: pharmacognosy, chemistry, and pharmacology of licorice. Yakugaku Zasshi 120, 849 862.
33. Tsukiyama, R., Katsura, H., Tokuriki, N. Kobayashi, M. (2002) Antibacterial activity of licochalcone A against spore-forming bacteria. Antimicrob Agents Chemother 46, 1226 1230.
34. Wang, D., Yu, L., Xiang, H., Fan, J., He, L., Guo, N., Feng, H. Deng, X. (2008) Global transcriptional profiles of Staphylococcus aureus treated with berberine chloride. FEMS Microbiol Lett 279, 217 225.
35. Worlitzsch, D., Kaygin, H., Steinhuber, A., Dalhoff, A., Botzenhart, K. Döring, G. (2001) Effects of amoxicillin, gentamicin, and moxifloxacin on the hemolytic activity of Staphylococcus aureus in vitro and in vivo. Antimicrob Agents Chemother 45, 196 202.