Improving the heat resistance of ribonuclease A by the addition of poly(N,N-diethylaminoethyl methacrylate)-graft-poly(ethylene glycol) (PEAMA-g-PEG)

Macromolecular Bioscience

Volumn 10, Issue 8, 2010, Pages 853-859

  Ganguli, Sumon ^a   Yoshimoto, Keitaro ^a   Tomita, Shunsuke ^a   Sakuma, Hiroshi ^a   Matsuoka, Tsuneyoshi ^a   Shiraki, Kentaro ^a   Nagasaki, Yukio ^a,b  

^a UNIVERSITY OF TSUKUBA   (Japan)

^b NATIONAL INSTITUTE FOR MATERIALS SCIENCE   (Japan)

Author keywords

Active site; Cationic graft copolymer; Enzyme; Heat resistance; Thermal inactivation

Indexed keywords

ACTIVE SITE; AFTER-TREATMENT; CATIONIC GRAFT COPOLYMER; CIRCULAR DICHROISM; ENZYMATIC ACTIVITIES; HEAT INACTIVATION; HEATING TEMPERATURES; INCUBATION TIME; IRREVERSIBLE INACTIVATION; N ,N-DIETHYLAMINOETHYL METHACRYLATE; NET CHARGES; POSITIVELY CHARGED; RIBONUCLEASE A; RNASE A; SPECTRAL ANALYSIS; THERMAL INACTIVATION;

COPOLYMERIZATION; DICHROISM; ENZYMES; ETHYLENE; ETHYLENE GLYCOL; GRAFT COPOLYMERS; GRAFTING (CHEMICAL); POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; SPECIFIC HEAT; SPECTRUM ANALYSIS; SPECTRUM ANALYZERS;

HEAT RESISTANCE;

COPOLYMER; MACROGOL; POLY(N,N DIETHYLAMINOETHYL METHACRYLATE); RIBONUCLEASE A; UNCLASSIFIED DRUG;

ARTICLE; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME STRUCTURE; HEAT TOLERANCE; HEAT TREATMENT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INCUBATION TIME; LIGHT SCATTERING; SPECTROSCOPY; STRUCTURE ANALYSIS; SURFACE CHARGE; SYNTHESIS; X RAY CRYSTALLOGRAPHY;

CIRCULAR DICHROISM; HOT TEMPERATURE; KINETICS; METHACRYLATES; MODELS, MOLECULAR; POLYETHYLENE GLYCOLS; RIBONUCLEASE, PANCREATIC; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 77956541777     PISSN: 16165187     EISSN: 16165195     Source Type: Journal    
DOI: 10.1002/mabi.200900432     Document Type: Article

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