Switching by Pulse Electric Field of the Elevated Enzymatic Reaction in the Core of Polyion Complex Micelles

Journal of the American Chemical Society

Volumn 125, Issue 50, 2003, Pages 15306-15307

  Harada, Atsushi ^a   Kataoka, Kazunori ^b  

^a Osaka Prefecture University   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ION; LYSOZYME;

ARTICLE; BINDING AFFINITY; BINDING SITE; COLORIMETRY; CONCENTRATION RESPONSE; ELECTRIC FIELD; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME MODIFICATION; ENZYME REGULATION; ENZYME STABILITY; ENZYME SUBSTRATE; MICELLE; MOLECULAR INTERACTION; REACTION ANALYSIS;

COLORIMETRY; EGG PROTEINS; KINETICS; MICELLES; MURAMIDASE; OLIGOSACCHARIDES; PEPTIDES; POLYETHYLENE GLYCOLS;

EID: 0348110661     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja038572h     Document Type: Article

References (19)

1. (a) Stayton, P. S.; Shimoboji, T.; Long, C.; Chilkoti, A.; Chen, G.; Harris, J. M.; Hoffman, A. S. Nature 1995, 378, 472-474.
2. (b) Ding, Z.; Fong, R. B.; Long, C. J.; Hoffman, A. S.; Stayton, P. S. Nature 2001, 411, 59-62.
3. (c) Shimoboji, T.; Ding, Z. L.; Stayton, P. S.; Hoffman, A. S. Bioconjugate Chem. 2002, 13, 915-919.
4. (a) Harris, J. M. In Poly(ethylene glycol) Chemistry; Harris, J. M., Ed. ; Plenum Press: New York, 1992; pp 1-14.
5. (b) Knauf, M. J.; Bell, D. P.; Hirtzer, P.; Luo, Z. P.; Young, J. D.; Katre, N. V. J. Biol. Chem. 1988, 263, 15064-15070.
6. (c) Gombotz, W. R.; Pettit, D. K. Bioconjugate Chem. 1995, 6, 332-351.
7. (a) Harada, A.; Kataoka, K. Macromolecules 1995, 28, 5294-5299.
8. (b) Harada, A.; Kataoka, K. Science 1999, 283, 65-67.
9. (c) Kataoka, K.; Harada, A.; Nagasaki, Y. Adv. Drug Delivery Rev. 2001, 47, 113-131.
10. (a) Harada, A.; Kataoka, K. Macromolecules 1998, 31, 288-294.
11. (b) Harada, A.; Kataoka, K. Langmuir 1999, 15, 4208-4212.
12. (c) Harada, A.; Kataoka, K. J. Controlled Release 2001, 72, 85-91.
13. (d) Harada, A.; Kataoka, K. Macromol. Symp. 172, 1-9.
14. PIC micelles entrapping lysozyme in the core were prepared by mixing lysozyme and PEG-P(Asp) in sodium phosphate buffer (10 mM, pH 7.4) at the equal residual molar ratio of Asp in PEG-P(Asp) to Lys + Arg in lysozyme. The formed PIC micelles had 50.8 ± 10.1 nm of average diameter in cumulant analysis of dynamic light-scattering measurements.
15. (a) Blake, C. C. F.; Johnson, L. N.; Mair, G. A.; North, A. C. T.; Phillips, D. C.; Sarma, V. R. Proc. R. Soc. 1967, B167, 378-388.
16. (b) Dahlquist, F. W.; Raftery, M. A. Biochemistry 1969, 8, 713.
17. (c) Raftery, M. A.; Dahlquist, F. W.; Parsons, S. M.; Wilcott, R. G. Proc. Natl. Acad. Sci. U.S.A. 1969, 62, 44.
18. A DC electric field was applied by soaking a pair of Pt wire electrodes in a quartz cell with the fixed distance of 1.0 cm.
19. Harada, A.; Kataoka, K., in preparation.