Deciphering the kinetic binding mechanism of dimeric ligands using a potent plasma-stable dimeric inhibitor of postsynaptic density protein-95 as an example

Journal of Biological Chemistry

Volumn 285, Issue 36, 2010, Pages 28252-28260

  Chi, Celestine N ^a   Bach, Anders ^b   Gottschalk, Marie ^b   Kristensen, Anders S ^b   Strømgaard, Kristian ^b   Jemth, Per ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; GENETIC ENGINEERING; LIGANDS; PROTEINS; RATE CONSTANTS;

BIDENTATE COMPLEXES; BINDING INTERACTION; BINDING REACTIONS; BIVALENT LIGANDS; CONFORMATIONAL TRANSITIONS; DIMERIC LIGANDS; DRUG DESIGN; ENZYME-SUBSTRATE INTERACTIONS; FLUORESCENCE POLARIZATION; FLUORIMETRY; INTRAMOLECULAR ASSOCIATION; ISOTHERMAL TITRATION CALORIMETRY; KINETIC BINDING; KINETIC MECHANISM; PDZ DOMAINS; POSTSYNAPTIC DENSITIES; POTENT INHIBITOR; PROTEIN ENGINEERING; STOPPED FLOW; TWO-STEP PROCESS;

BINDING SITES;

POSTSYNAPTIC DENSITY PROTEIN 95; LIGAND; MEMBRANE PROTEIN; OLIGOPEPTIDE; PROTEIN BINDING;

ARTICLE; BINDING AFFINITY; BINDING KINETICS; BINDING SITE; CONTROLLED STUDY; FLUORESCENCE POLARIZATION; FLUOROMETRY; ISOTHERMAL TITRATION CALORIMETRY; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN INTERACTION; AMINO ACID SEQUENCE; ANTAGONISTS AND INHIBITORS; BLOOD; CHEMICAL STRUCTURE; CHEMISTRY; DIMERIZATION; KINETICS; METABOLISM; PDZ DOMAIN; PROTEIN STABILITY;

AMINO ACID SEQUENCE; DIMERIZATION; KINETICS; LIGANDS; MEMBRANE PROTEINS; MODELS, MOLECULAR; OLIGOPEPTIDES; PDZ DOMAINS; PROTEIN BINDING; PROTEIN STABILITY;

EID: 77956239256     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.124040     Document Type: Article

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