DMSO and betaine greatly improve amplification of GC-rich constructs in de novo synthesis

PLoS ONE

Volumn 5, Issue 6, 2010, Pages

  Jensen, Michael A ^a   Fukushima, Marilyn ^a   Davis, Ronald W ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

B RAF KINASE; BETAINE; DIMETHYL SULFOXIDE; LIGASE; SOMATOMEDIN B RECEPTOR;

ARTICLE; CONTROLLED STUDY; DNA REPLICATION; GC RICH SEQUENCE; GENE AMPLIFICATION; GENE SYNTHESIS; INTERMETHOD COMPARISON; LIGASE CHAIN REACTION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN ASSEMBLY; POLYMERASE CHAIN REACTION; PROTEIN SECONDARY STRUCTURE;

BASE SEQUENCE; BETAINE; DIMETHYL SULFOXIDE; DNA PRIMERS; GC RICH SEQUENCE; POLYMERASE CHAIN REACTION; PROTO-ONCOGENE PROTEINS B-RAF; RECEPTOR, IGF TYPE 2;

EID: 77956195631     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0011024     Document Type: Article

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