Amplification of GC-rich genes by following a combination strategy of primer design, enhancers and modified PCR cycle conditions

Molecular and Cellular Probes

Volumn 21, Issue 4, 2007, Pages 303-307

  Sahdev, Sudhir ^a   Saini, Shalini ^a   Tiwari, Prabhakar ^a   Saxena, Sanjeev ^a   Singh Saini, Kulvinder ^a  

^a RANBAXY RESEARCH LABORATORIES   (India)

Author keywords

Betaine; cDNA; cSRC kinase; Dimethyl sulfoxide (DMSO); Insulin receptor; Primer free energy ( g)

Indexed keywords

COMPLEMENTARY DNA; INSULIN RECEPTOR; RNA;

ARTICLE; COMPUTER MODEL; DNA SYNTHESIS; ENERGY TRANSFER; GENE AMPLIFICATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA ANALYSIS; TEMPERATURE MEASUREMENT;

DIMERIZATION; DNA PRIMERS; DNA, COMPLEMENTARY; ENHANCER ELEMENTS (GENETICS); GC RICH SEQUENCE; HT29 CELLS; HUMANS; NUCLEIC ACID CONFORMATION; POLYMERASE CHAIN REACTION; RECEPTOR, INSULIN; SRC-FAMILY KINASES; THERMODYNAMICS;

EID: 34248199933     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2007.03.004     Document Type: Article

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