Optimal conditions and specific characteristics of Vent exo- DNA polymerase in ligation-mediated polymerase chain reaction protocols

Biochemistry and Cell Biology

Volumn 83, Issue 2, 2005, Pages 147-165

  Vigneault, François ^a,b   Drouin, Régen ^a,b  

^a LAVAL UNIVERSITY   (Canada)

^b CENTRE HOSPITALIER UNIVERSITAIRE DE SHERBROOKE   (Canada)

Author keywords

DNA sequence context; Ligation mediated polymerase chain reaction (PCR); PCR buffer; Pfu exo DNA polymerase; Vent exo DNA polymerase

Indexed keywords

BIOCHEMISTRY; CELLS; ENZYMES; MAGNESIUM COMPOUNDS; OPTIMIZATION;

LIGATION-MEDIATED PCR; OPTIMAL DOSAGE; PCR AMPLIFICATION; POLYMERASE CHAIN REACTION (PCR);

DNA;

DNA; DNA POLYMERASE; GENOMIC DNA; MAGNESIUM SULFATE;

ARTICLE; DNA DETERMINATION; ENZYME ACTIVITY; ENZYME MECHANISM; GENE AMPLIFICATION; LIGATION; POLYMERASE CHAIN REACTION; PYROCOCCUS FURIOSUS; TECHNIQUE; THERMOCOCCUS; THERMOCOCCUS LITORALIS; THERMUS AQUATICUS;

DNA; DNA LIGASES; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; FRAGILE X MENTAL RETARDATION PROTEIN; GC RICH SEQUENCE; HUMANS; JNK MITOGEN-ACTIVATED PROTEIN KINASES; LYMPHOCYTES; MALE; NERVE TISSUE PROTEINS; POLYMERASE CHAIN REACTION; PYROCOCCUS FURIOSUS; RNA-BINDING PROTEINS; TAQ POLYMERASE; TEMPERATURE; TEMPLATES, GENETIC; THERMOCOCCUS; TUMOR SUPPRESSOR PROTEIN P53;

PYROCOCCUS FURIOSUS; THERMOCOCCUS LITORALIS; THERMUS AQUATICUS;

EID: 23444436509     PISSN: 08298211     EISSN: None     Source Type: Journal    
DOI: 10.1139/o04-134     Document Type: Article

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