Structural and kinetic studies on the Ser101Ala variant of choline oxidase: Catalysis by compromise

Archives of Biochemistry and Biophysics

Volumn 501, Issue 2, 2010, Pages 207-213

  Finnegan, Steffan ^a   Yuan, Hongling ^a   Wang, Yuan Fang ^a   Orville, Allen M ^b   Weber, Irene T ^a   Gadda, Giovanni ^a  

^a GEORGIA STATE UNIVERSITY   (United States)

^b BROOKHAVEN NATIONAL LABORATORY   (United States)

Author keywords

Choline; Flavoprotein; Kinetics; Mechanism; Oxidase; Reductive half reaction

Indexed keywords

ALCOHOL; CARBOXYLIC ACID; CHOLINE; CHOLINE OXIDASE; FLAVOPROTEIN; HYDROXYL GROUP; SER101ALA; UNCLASSIFIED DRUG; ALCOHOL DEHYDROGENASE; BACTERIAL PROTEIN; MUTANT PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ENZYME STRUCTURE; KINETICS; MUTAGENESIS; MUTATION; PRIORITY JOURNAL; TURNOVER TIME; WILD TYPE; AMINO ACID SUBSTITUTION; ARTHROBACTER; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETIC VARIABILITY; GENETICS; METABOLISM; SITE DIRECTED MUTAGENESIS; STATIC ELECTRICITY; X RAY CRYSTALLOGRAPHY;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SUBSTITUTION; ARTHROBACTER; BACTERIAL PROTEINS; CHOLINE; CRYSTALLOGRAPHY, X-RAY; GENETIC VARIATION; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; RECOMBINANT PROTEINS; STATIC ELECTRICITY;

EID: 77956177377     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2010.06.014     Document Type: Article

References (29)

1. Gadda G. Biochemistry 2008, 47:13745-13753.
2. Waditee R., Bhuiyan N.H., Hirata E., Hibino T., Tanaka Y., Shikata M., Takabe T. J. Biol. Chem. 2007, 282:34185-34193.
3. Quan R., Shang M., Zhang H., Zhao Y., Zhang J. Plant Biotechnol. J. 2004, 2:477-486.
4. Sakamoto A., Valverde R., Alia, Chen T.H., Murata N. Plant J. 2000, 22:449-453.
5. Holmstrom K.O., Somersalo S., Mandal A., Palva T.E., Welin B. J. Exp. Bot. 2000, 51:177-185.
6. Fan F., Gadda G. J. Am. Chem. Soc. 2005, 127:2067-2074.
7. Fan F., Germann M.W., Gadda G. Biochemistry 2006, 45:1979-1986.
8. Gadda G. Biochim. Biophys. Acta 2003, 1646:112-118.
9. Ghanem M., Fan F., Francis K., Gadda G. Biochemistry 2003, 42:15179-15188.
10. Quaye O., Cowins S., Gadda G. J. Biol. Chem. 2009, 284:16990-16997.
11. Quaye O., Lountos G.T., Fan F., Orville A.M., Gadda G. Biochemistry 2008, 47:243-256.
12. Quaye O., Gadda G. Arch. Biochem. Biophys. 2009, 489:10-14.
13. Rungsrisuriyachai K., Gadda G. Biochemistry 2008, 47:6762-6769.
14. Finnegan S., Agniswamy J., Weber I.T., Gadda G. Biochemistry 2010, 49:2952-2961.
15. Finnegan S., Gadda G. Biochemistry 2008, 47:13850-13861.
16. Ghanem M., Gadda G. Biochemistry 2005, 44:893-904.
17. Ghanem M., Gadda G. Biochemistry 2006, 45:3437-3447.
18. Gadda G., Powell N.L., Menon P. Arch. Biochem. Biophys. 2004, 430:264-273.
19. Gadda G., Fan F., Hoang J.V. Arch. Biochem. Biophys. 2006, 451:182-187.
20. Fan F., Ghanem M., Gadda G. Arch. Biochem. Biophys. 2004, 421:149-158.
21. Otwinowski Z., Minor W. Methods Enzymol. 1997, 276:307-326.
22. McCoy A.J., Grosse-Kunstleve R.W., Adams P.D., Winn M.D., Storoni L.C., Read R.J. J. Appl. Crystallogr. 2007, 40:658-674.
23. Berman H.M., Westbrook J., Feng Z., Gilliland G., Bhat T.N., Weissig H., Shindyalov I.N., Bourne P.E. Nucleic Acids Res. 2000, 28:235-242.
24. Murshudov G.N., Vagin A.A., Dodson E.J. Acta Crystallogr. D Biol. Crystallogr. 1997, 53:240-255.
25. Potterton E., Briggs P., Turkenburg M., Dodson E. Acta Crystallogr. D Biol. Crystallogr. 2003, 59:1131-1137.
26. Emsley P., Cowtan K. Acta Crystallogr. D Biol. Crystallogr. 2004, 60:2126-2132.
27. DeLano W.L. The Pymol Molecular Graphics System 2002, San Carlos, CA.
28. Allison R.D., Purich D.L. Methods Enzymol. 1979, 63:3-22.
29. Orville A.M., Lountos G.T., Finnegan S., Gadda G., Prabhakar R. Biochemistry 2009, 48:720-728.