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There is a small difference between the sum spectrum and the spectrum of the mixture. It is worth noting that even at λ = 600 nm, at which the absorbance should ideally be 0.0, the absorbance is actually very close to zero Figure 3b but not exactly 0.0. The very negligible absorbance arises due to scattering, and the intensity of scattering increases towards lower wavelengths. In the calculated spectrum, the effect of scattering is actually counted twice in the process of mathematical summation of the two individual spectra which might have contributed to the observed minor difference
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There is a small difference between the sum spectrum and the spectrum of the mixture. It is worth noting that even at λ = 600 nm, at which the absorbance should ideally be 0.0, the absorbance is actually very close to zero (Figure 3b) but not exactly 0.0. The very negligible absorbance arises due to scattering, and the intensity of scattering increases towards lower wavelengths. In the calculated spectrum, the effect of scattering is actually counted twice in the process of mathematical summation of the two individual spectra which might have contributed to the observed minor difference.
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To get the sole contribution of D1 to the absorption at λ=312 nm, the spectrum was subtracted from the A1 spectrum
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