An amphiphilic folding molecule that undergoes an irreversible conformational change [22]

Journal of the American Chemical Society

Volumn 121, Issue 11, 1999, Pages 2639-2640

  Nguyen, John Q ^a   Iverson, Brent L ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AROMATIC COMPOUND; SYNTHETIC PEPTIDE;

CHEMICAL STRUCTURE; CONFORMATION; CONFORMATIONAL TRANSITION; ELECTRON TRANSPORT; HEATING; KINETICS; LETTER; PEPTIDE ANALYSIS; PROTON NUCLEAR MAGNETIC RESONANCE; ULTRAVIOLET SPECTROSCOPY; VISCOSITY;

EID: 0033599480     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9838920     Document Type: Letter

References (18)

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13. Protein Solutions Dyna Pro-801 Molecular Sizing Instrument (2 mM solution of aedamer 1 in 20 mM phosphate buffer, filtered through a 0.02 mm 13 mm Whatman Anodisc 13).
14. A 56% hypochromism corresponds to an absorbance of the folded aedamer 1 in aqueous buffer that is 44% of that observed for the same concentration of unfolded aedamer in 2% CTAB.
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16. Because of aggregation, it is impossible to rule out rigorously that the hypochromism and charge-transfer bands are derived in part from intermolecular stacking interactions. However, the similarity in spectral results for 1 and the monomeric 2 can be used to argue against intermolecular stacking effects playing a major role in the spectra observed for 1.
17. On the basis of the integration of the HPLC peaks, the material recovered after heating during different runs amounts to an average of 40 ± 10% of that expected. The remainder of the tangled aggregate material was likely irreversibly adsorbed onto the HPLC precolumn.
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