Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources

Protein Engineering, Design and Selection

Volumn 23, Issue 7, 2010, Pages 567-577

  Cho, Yong Ku ^a   Shusta, Eric V ^a  

^a University of Wisconsin Madison   (United States)

Author keywords

antibody; cell lysate; library selection; yeast display

Indexed keywords

AFFINITY REAGENTS; ANTIBODY LIBRARY; AQUEOUS SOLUTIONS; BIOLOGICAL APPLICATIONS; BIOTINYLATIONS; CELL LYSATE; CELL LYSATES; CELLULAR COMPONENTS; HETEROLOGOUS EXPRESSION; HIGH-THROUGHPUT; IMMUNOPRECIPITATIONS; INTRACELLULAR PROTEINS; LYSATES; MAMMALIAN CELLS; MEMBRANE PROTEINS; PHYSIOLOGICAL CONDITION; PLASMA MEMBRANE PROTEIN; PROTEOMICS; SINGLE-CHAIN ANTIBODIES; TARGET CELLS; TRANSFERRIN RECEPTORS; YEAST DISPLAY;

ANTIGENS; BIOLOGICAL MEMBRANES; CELL CULTURE; CELL MEMBRANES; MAMMALS; MOLECULAR BIOLOGY; PURIFICATION; SOAPS (DETERGENTS); SOLUTIONS; YEAST;

ANTIBODIES;

ANTIGEN; CELL PROTEIN; DETERGENT; MEMBRANE PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TRANSFERRIN RECEPTOR;

ANTIBODY LABELING; ANTIBODY LIBRARY; ANTIBODY SCREENING; ANTIGEN EXPRESSION; ANTIGEN PURIFICATION; ARTICLE; BIOTINYLATION; CELL LYSATE; CELL MEMBRANE; CONTROLLED STUDY; IMMUNOPRECIPITATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SOLUBILITY; YEAST;

ANIMALS; BLOTTING, WESTERN; CELL EXTRACTS; CELL LINE; EPITOPES; FLOW CYTOMETRY; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; MEMBRANE PROTEINS; PEPTIDE LIBRARY; RATS; RECEPTORS, TRANSFERRIN; SACCHAROMYCES CEREVISIAE; SINGLE-CHAIN ANTIBODIES;

MAMMALIA;

EID: 77955969564     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzq029     Document Type: Article

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