A novel method for making human monoclonal antibodies

Journal of Autoimmunity

Volumn 35, Issue 2, 2010, Pages 130-134

  Fraussen, J ^a   Vrolix, K ^b   Martinez Martinez, P ^b   Losen, M ^b   Meulemans, E ^c   De Baets, M H ^a,b   Stinissen, P ^a   Somers, V ^a  

^a HASSELT UNIVERSITY   (Belgium)

^b MAASTRICHT UNIVERSITY   (Netherlands)

^c MAASTRICHT UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

B cell immortalization; B cell spectratyping.; Epstein Barr virus; Monoclonal antibodies

Indexed keywords

AUTOANTIGEN; CD22 ANTIGEN; CPG 2006; HUMAN MONOCLONAL ANTIBODY; IMMUNOGLOBULIN G; INTERLEUKIN 2; OLIGODEOXYNUCLEOTIDE; UNCLASSIFIED DRUG;

ANTIBODY PRODUCTION; ARTICLE; AUTOIMMUNE DISEASE; B LYMPHOCYTE; CELL IMMORTALIZATION; CONTROLLED STUDY; DRUG SYNTHESIS; EPSTEIN BARR VIRUS INFECTION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SUPERNATANT;

ANTIBODIES, MONOCLONAL; ANTIGENS, CD22; B-LYMPHOCYTES; CELL CULTURE TECHNIQUES; CELL TRANSFORMATION, VIRAL; CLONE CELLS; DNA; EPSTEIN-BARR VIRUS INFECTIONS; HERPESVIRUS 4, HUMAN; HUMANS; HYBRIDOMAS; IMMUNOGLOBULIN G; INTERLEUKIN-2; OLIGODEOXYRIBONUCLEOTIDES;

EID: 77955921386     PISSN: 08968411     EISSN: 10959157     Source Type: Journal    
DOI: 10.1016/j.jaut.2010.05.001     Document Type: Article

References (38)

1. Martin D.R., Marlowe R.L., Ahearn J.M. Determination of the role for CD21 during Epstein-Barr virus infection of B-lymphoblastoid cells. J Virol 1994, 68(8):4716-4726.
2. Somers K., Govarts C., Stinissen P., Somers V. Multiplexing approaches for autoantibody profiling in multiple sclerosis. Autoimmun Rev 2009, 8(7):573-579.
3. Somers V., Govarts C., Somers K., Hupperts R., Medaer R., Stinissen P. Autoantibody profiling in multiple sclerosis reveals novel antigenic candidates. J Immunol 2008, 180(6):3957-3963.
4. Govarts C., Somers K., Hupperts R., Stinissen P., Somers V. Analysis of antibody reactivity in paired cerebrospinal fluid and serum of a relapsing remitting multiple sclerosis patient. Autoimmunity 2009, 42(8):699-704.
5. Carter P.J. Potent antibody therapeutics by design. Nat Rev Immunol 2006, 6(5):343-357.
6. Kohler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975, 256(5517):495-497.
7. Levy R., Dilley J. Rescue of immunoglobulin secretion from human neoplastic lymphoid cells by somatic cell hybridization. Proc Natl Acad Sci U.S.A 1978, 75(5):2411-2415.
8. McCafferty J., Griffiths A.D., Winter G., Chiswell D.J. Phage antibodies: filamentous phage displaying antibody variable domains. Nature 1990, 348(6301):552-554.
9. Olsson L., Kaplan H.S. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc Natl Acad Sci U.S.A 1980, 77(9):5429-5431.
10. Meijer P.J., Andersen P.S., Haahr H.M., Steinaa L., Jensen A., Lantto J., et al. Isolation of human antibody repertoires with preservation of the natural heavy and light chain pairing. J Mol Biol 2006, 358(3):764-772.
11. Rosen A., Gergely P., Jondal M., Klein G., Britton S. Polyclonal Ig production after Epstein-Barr virus infection of human lymphocytes in vitro. Nature 1977, 267(5606):52-54.
12. Steinitz M., Klein G., Koskimies S., Makel O. EB virus-induced B lymphocyte cell lines producing specific antibody. Nature 1977, 269(5627):420-422.
13. Traggiai E., Becker S., Subbarao K., Kolesnikova L., Uematsu Y., Gismondo M.R., et al. An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus. Nat Med 2004, 10(8):871-875.
14. Funaro A., Gribaudo G., Luganini A., Ortolan E., Lo B.N., Vicenzi E., et al. Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells. BMC Biotechnol 2008, 8:85.
15. van Dongen J.J., Langerak A.W., Bruggemann M., Evans P.A., Hummel M., Lavender F.L., et al. Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia 2003, 17(12):2257-2317.
16. Cross A.H., Trotter J.L., Lyons J. B cells and antibodies in CNS demyelinating disease. J Neuroimmunol 2001, 112(1-2):1-14.
17. Kabat E.A., Glusman M., Knaub V. Quantitative estimation of the albumin and gamma-globulin in normal and pathological cerebrospinal fluid by immunochemical methods. Am J Med 1948, 4:653-662.
18. Graus Y.F., De Baets M.H., Parren P.W., Berrih-Aknin S., Wokke J., Breda Vriesman P.J., et al. Human anti-nicotinic acetylcholine receptor recombinant Fab fragments isolated from thymus-derived phage display libraries from myasthenia gravis patients reflect predominant specificities in serum and block the action of pathogenic serum antibodies. J Immunol 1997, 158(4):1919-1929.
19. Vrolix K., Fraussen J., Molenaar P.C., Losen M., Somers V., Stinissen P., et al. The auto-antigen repertoire in myasthenia gravis. Autoimmunity 2010.
20. Weissmann G. Rheumatoid arthritis and systemic lupus erythematosus as immune complex diseases. Bull NYU Hosp Jt Dis 2009, 67(3):251-253.
21. Hurst J., von Landenberg P. Toll-like receptors and autoimmunity. Autoimmun Rev 2008, 7(3):204-208.
22. Bernasconi N.L., Traggiai E., Lanzavecchia A. Maintenance of serological memory by polyclonal activation of human memory B cells. Science 2002, 298(5601):2199-2202.
23. Hanten J.A., Vasilakos J.P., Riter C.L., Neys L., Lipson K.E., Alkan S.S., et al. Comparison of human B cell activation by TLR7 and TLR9 agonists. BMC Immunol 2008, 9:39.
24. Hartmann G., Weeratna R.D., Ballas Z.K., Payette P., Blackwell S., Suparto I., et al. Delineation of a CpG phosphorothioate oligodeoxynucleotide for activating primate immune responses in vitro and in vivo. J Immunol 2000, 164(3):1617-1624.
25. Schlaepfer E., Audige A., von Beust B., Manolova V., Weber M., Joller H., et al. CpG oligodeoxynucleotides block human immunodeficiency virus type 1 replication in human lymphoid tissue infected ex vivo. J Virol 2004, 78(22):12344-12354.
26. Luganini A., Caposio P., Landolfo S., Gribaudo G. Phosphorothioate-modified oligodeoxynucleotides inhibit human cytomegalovirus replication by blocking virus entry. Antimicrob Agents Chemother 2008, 52(3):1111-1120.
27. Kato A., Homma T., Batchelor J., Hashimoto N., Imai S., Wakiguchi H., et al. Interferon-alpha/beta receptor-mediated selective induction of a gene cluster by CpG oligodeoxynucleotide 2006. BMC Immunol 2003, 4:8.
28. Ladell K., Dorner M., Zauner L., Berger C., Zucol F., Bernasconi M., et al. Immune activation suppresses initiation of lytic Epstein-Barr virus infection. Cell Microbiol 2007, 9(8):2055-2069.
29. Liang H., Nishioka Y., Reich C.F., Pisetsky D.S., Lipsky P.E. Activation of human B cells by phosphorothioate oligodeoxynucleotides. J Clin Invest 1996, 98(5):1119-1129.
30. Lanzavecchia A, inventors; Monoclonal antibody production by EBV transformation of B cells. WO/2004/076677. 2004 Sept. 10.
31. Muraguchi A., Kehrl J.H., Longo D.L., Volkman D.J., Smith K.A., Fauci A.S. Interleukin 2 receptors on human B cells. Implications for the role of interleukin 2 in human B cell function. J Exp Med 1985, 161(1):181-197.
32. Bourke E., Bosisio D., Golay J., Polentarutti N., Mantovani A. The toll-like receptor repertoire of human B lymphocytes: inducible and selective expression of TLR9 and TLR10 in normal and transformed cells. Blood 2003, 102(3):956-963.
33. Sugimoto M., Tahara H., Ide T., Furuichi Y. Steps involved in immortalization and tumorigenesis in human B-lymphoblastoid cell lines transformed by Epstein-Barr virus. Cancer Res 2004, 64(10):3361-3364.
34. Fraussen J., Vrolix K., Martinez-Martinez P., Losen M., De Baets M.H., Stinissen P., et al. B cell characterization and reactivity analysis in multiple sclerosis. Autoimmun. Rev. 2009, 8(8):654-658.
35. Chong Y., Ikematsu H., Yamaji K., Nishimura M., Nabeshima S., Kashiwagi S., et al. CD27(+) (memory) B cell decrease and apoptosis-resistant CD27(-) (naive) B cell increase in aged humans: implications for age-related peripheral B cell developmental disturbances. Int Immunol 2005, 17(4):383-390.
36. Fraussen J., Vrolix K., Martinez-Martinez P., Hupperts R., Van Diepen A., Meulemans E., et al. Antibody-producing monoclonal B cell lines from multiple sclerosis patients obtained by B cell immortalization. Clin Immunol 2009, 131:S129-S130.
37. Fraussen J., Martinez P., De Baets M., Van Diepen A., Meulemans E., Stinissen P., et al. Characterizing autoantibodies in multiple sclerosis by B cell immortalization. J Neuroimmunol 2008, 203(2):183-184.
38. Vrolix K., Fraussen J., Meulemans E., Becker P., Phernambucq M., Somers V., et al. Monoclonal antibody production by immortalization of B cells from the thymus of myasthenia gravis patients. J Neuroimmunol 2008, 203(2):258.