Human Anti-Nicotinic Acetylcholine Receptor Recombinant Fab Fragments Isolated from Thymus-Derived Phage Display Libraries from Myasthenia Gravis Patients Reflect Predominant Specificities in Serum and Block the Action of Pathogenic Serum Antibodies

Journal of Immunology

Volumn 158, Issue 4, 1997, Pages 1919-1929

  Graus, Yvo F ^a,b   De Baets, Marc H ^b   Parren, Paul W H I ^a   Berrih Aknin, Sonia ^c   Wokke, John ^d   Van Breda Vriesman, Peter J ^b   Burton, Dennis R ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b MAASTRICHT UNIVERSITY   (Netherlands)

^c CENTRE CHIRURGICAL MARIE LANNELONGUE   (France)

^d UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISERUM; AUTOANTIBODY; AUTOANTIGEN; BLOCKING ANTIBODY; CROSS LINKING REAGENT; IMMUNOGLOBULIN F(AB) FRAGMENT; NICOTINIC RECEPTOR; RECOMBINANT PROTEIN;

ADULT; AMINO ACID SEQUENCE; ANTIBODY SPECIFICITY; ARTICLE; BINDING COMPETITION; BLOOD; CHEMISTRY; COLIPHAGE; EPITOPE MAPPING; FEMALE; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; MALE; METABOLISM; MIDDLE AGED; MOLECULAR GENETICS; MYASTHENIA GRAVIS; NUCLEOTIDE SEQUENCE; SEQUENCE ANALYSIS; THYMUS; VIROLOGY;

ADULT; AMINO ACID SEQUENCE; ANTIBODIES, BLOCKING; ANTIBODY SPECIFICITY; AUTOANTIBODIES; AUTOANTIGENS; BASE SEQUENCE; BINDING, COMPETITIVE; COLIPHAGES; CROSS-LINKING REAGENTS; EPITOPE MAPPING; FEMALE; HUMANS; IMMUNE SERA; IMMUNOGLOBULIN FAB FRAGMENTS; MALE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; MYASTHENIA GRAVIS; RECEPTORS, NICOTINIC; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS; THYMUS GLAND;

EID: 0031568670     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (56)

1. Keesey, J., J. Lindstrom, and H. Cokely. 1977. Anti-acetylcholine receptor antibody in neonatal myasthenia gravis. N. Engl. J. Med. 296:55 (Lett.).
2. Toyka, K., D. Brachman, A. Pestronk, and I. Kao. 1975. Myasthenia gravis: passive transfer from man to mouse. Science 190:397.
3. Tzartos, S., S. Hochschwender, P. Vasquez, and J. Lindstrom. 1987. Passive transfer of experimental autoimmune myasthenia gravis by monoclonal antibodies to the main immunogenic region of the acetylcholine receptor. J. Neuroimmunol. 15:185.
4. Newsom, D. J., S. G. Wilson, A. Vincent, and C. D. Ward. 1979. Long-term effects of repeated plasma exchange in myasthenia gravis. Lancet 1:464.
5. Vincent, A., P. Whiting, M. Schluep, F. Heidenreich, B. Lang, A. Roberts, N. Willcox, and J. Newsom Davis. 1987. Antibody heterogeneity and specificity in myasthenia gravis. Ann. NY Acad. Sci. 505:106.
6. Heidenreich, F., A. Vincent, and J. Newsom Davis. 1988. Differences in fine specificity of anti-acetylcholine receptor antibodies between subgroups of spontaneous myasthenia gravis of recent onset, and of penicillamine induced myasthenia. Autoimmunity 2:31.
7. Tzartos, S., M. Seybold, and J. Lindstrom. 1982. Specificities of antibodies to acetylcholine receptors in sera from myasthenia gravis patients measured by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 79:188.
8. Graus, Y., F. Meng, A. Vincent, P. van Breda Vriesman, and M. de Baets. 1995. Sequence analysis of anti-AChR antibodies in experimental autoimmune myasthenia gravis. J. Immunol. 154:6382.
9. Lennon, V., and G. Griesmann. 1989. Evidence against acetylcholine receptor having a main immunogenic region as target for autoantibodies in myasthenia gravis. Neurology 39:1009.
10. Blair, D., D. Richman, C. Taves and S. Koethe. 1986. Monoclonal antibodies to acetylcholine receptor secreted by human X human hybridomas derived from lymphocytes of a patient with myasthenia gravis. Immunol. Invest. 15:351.
11. Cardona, A., H. Garchon, B. Vernet der Garabedian, E. Morel, P. Gajdos, and J. F. Bach. 1994. Human IgG monoclonal autoantibodies against muscle acetylcholine receptor: direct evidence for clonal heterogeneity of the antiself humoral response in myasthenia gravis. J. Neuroimmunol. 53:9.
12. Kamo, I., S. Furukawa, A. Tada, Y. Mano, Y. Iwasaki, T, Furuse, N. Ito, K. Hayashi, and E. Satoyoshi. 1982. Monoclonal antibody to acetylcholine receptor: cell line established from thymus of patient with myasthenia gravis. Science 215:995.
13. Lefvert, A., G. Holm, and R. Pirskanen. 1987. Autoantiidiotypic antibodies in myasthenia gravis. Ann. NY Acad. Sci. 505:133.
14. Tindall, R. 1981. Humoral immunity in myasthenia gravis: biochemical characterization of acquired antireceptor antibodies and clinical correlations. Ann. Neurol. 10:437.
15. Vincent, A., and J. Newsom Davis. 1982. Acetylcholine receptor antibody characteristics in myasthenia gravis. I. Patients with generalized myasthenia or disease restricted to ocular muscles. Clin. Exp. Immunol. 49:257.
16. Loutrari, H., S. Tzartos, and T. Claudio. 1992. Use of Torpedo-mouse hybrid acetylcholine receptors reveals immunodominance of the alpha subunit in myasthenia gravis antisera. Eur. J. Immunol. 22:2949.
17. Zielinski, A., T. Brenner, and O. Abramsky. 1982. Binding of myasthenia gravis antibodies to different acetylcholine receptor preparations. Isr. J. Med. Sci. 18: 483.
18. Williams, C., and V. Lennon. 1986. Thymic B lymphocyte clones from patients with myasthenia gravis secrete monoclonal striational autoantibodies reacting with myosin, alpha actinin, or actin. J. Exp. Med. 164:1043.
19. Williams, C., J. Hay, T. Huiatt, and V. Lennon. 1992. Paraneoplastic IgG striational autoantibodies produced by clonal thymic B cells and in serum of patients with myasthenia gravis and thymoma react with titin. Lab. Invest. 66:331.
20. Scadding, G., A. Vincent, J. Newsom Davis, and K. Henry. 1981. Acetylcholine receptor antibody synthesis by thymic lymphocytes, correlation with thymic histology. Neurology 31:935.
21. Kuks, J., H. Oosterhuis, P. Limburg, and T. The. 1991. Anti-acetylcholine receptor antibodies decrease after thymectomy in patients with myasthenia gravis: clinical correlations. J. Autoimmun. 4:197.
22. Kuks, J., P. Limburg, H. Oosterhuis, and T. The. 1992. Antibodies to acetylcholine receptors in myasthenia gravis: in vitro synthesis by peripheral blood lymphocytes before and after thymectomy. Clin. Exp. Immunol. 87:246.
23. Burton, D., and C. Barbas III. 1994. Human antibodies from combinatorial libraries. Adv. Immunol. 57:191.
24. Winter, G., A. Griffiths, R. Hawkins, and H. Hoogenboom. 1994. Making antibodies by phage display technology. Annu. Rev. Immunol. 12:433.
25. Abrams, P., J. Rossio, H. Stevenson, and K. Foon. 1986. Optimal strategies for developing human-human monoclonal antibodies. Methods Enzymol. 121:107.
26. Portolano, S., G. Chaznbalk, P. Seto, B. Rapoport, and S. McLachlan. 1992. Recognition by recombinant autoimmune thyroid disease-derived Fab fragments of a dominant conformational epitope on human thyroid peroxidase. J. Clin. Invest. 90:720.
27. Chazenbalk, G., S. Portolano, D. Russo, J. Hutchison, B. Rapoport, and S. McLachlan. 1993. Human organ-specific autoimmune disease: molecular cloning and expression of an autoantibody gene repertoire for a major autoantigen reveals an antigenic immunodominant region and restricted immunoglobulin gene usage in the target organ. J. Clin. Invest. 92:62.
28. Portolano, S., S. McLachlan, and B. Rapoport. 1993. High affinity, thyroid-specific human autoantibodies displayed on the surface of filamentous phage use V genes similar to other autoantibodies. J. Immunol. 151:2839.
29. Cha, S., P. Leung, M. Gershwin, M. Fletcher, A. Ansari, and R. Coppel. 1993. Combinatorial autoantibodies to dihydrolipoamide acetyltransferase, the major autoantigen of primary biliary cirrhosis. Proc. Natl. Acad. Sci. USA 90:2527.
30. Cha, S., P. Leung, R. Coppel, J. Van de Water, A. Ansari, and M. Gershwin. 1994. Heterogeneity of combinatorial human autoantibodies against PDC-E2 and biliary epithelial cells in patients with primary biliary cirrhosis. Hepatology 20: 574.
31. Hexham, J., L. Partridge, J. Furmaniak, V. Petersen, J. Colls, C. Pegg, B. Rees Smith, and D. Burton. 1994. Cloning and characterisation of TPO autoantibodies using combinatorial phage display libraries. Autoimmunity 17:167.
32. Jaume, P., S. Portolano, M. Prummel, S. McLachlan, and B. Rapoport. 1994. Molecular cloning and characterization of genes for antibodies generated by orbital tissue infiltrating B-cells in Graves opthalmopathy. J. Clin. Endocrinol. Metab. 78:348.
33. Barbas, S., H. Ditzel, E. Salonen, W. Yang, G. Silverman, and D. Burton. 1995. Human autoantibody recognition of DNA. Proc. Natl. Acad. Sci. USA 92:2529.
34. Luther, M., R. Schoepfer, P. Whiting, B. Casey, Y. Blatt, M. Montal, M. Montal, and J. Linstrom. 1989. A muscle acetylcholine receptor is expressed in the human cerebellar medulloblastoma cell line TE671. J. Neurosci. 9:1082.
35. Persson, M., R. Caothien, and D. Burton. 1991. Generation of diverse high affinity human monoclonal antibodies by repertoire cloning. Proc. Natl. Acad. Sci. USA 88:2432.
36. Williamson, R., R. Burioni, P. Sanna, C. Partridge, C. Barbas III, and D. Burton. 1993. Human monoclonal antibodies against a plethora of viral pathogens from single combinatorial libraries. Proc. Natl. Acad. Sci. USA 90:4141.
37. Barbas, C., III, A. Kang, R. Lerner, and S. Benkovic. 1991. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc. Natl. Acad. Sci. USA 88:7978.
38. Burton, D., C. Barbas III, M. Persson, S. Koening, R. Chanock, and R. Lerner. 1991. A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals. Proc. Natl. Acad. Sci. USA 88:10134.
39. Tzartos, S., and J. Lindstrom. 1980. Monoclonal antibodies used to probe acetylcholine receptor structure: localization of the main immunogenic region and detection of similarities between subunits. Proc. Natl. Acad. Sci. USA 77:755.
40. Tzartos, S. J., and M. S. Remoundos. 1992. Precise epitope mapping of monoclonal antibodies to the cytoplasmic side of the acetylcholine receptor alpha subunit: dissecting a potentially myasthenogenic epitope. Eur. J. Biochem. 207:915.
41. Barbas, C., III, J. Crowe, D. Cababa, T. Jones, S. Zebedee, B. Murphy, R. Chanock, and D. Burton. 1992. Human monoclonal Fab fragments derived from a combinatorial library bind to syncytial virus F glycoprotein and neutralize infectivity. Proc. Natl. Acad. Sci. USA 89:10164.
42. Graus, Y., J. Verschuuren, F. Spaans, F. Jennekens, P. van Breda Vriesman, and M. De Baets. 1993. Age-related resistance to experimental autoimmune myasthenia gravis in rats. J. Immunol. 150:4093.
43. + flux into TE671 cell line. J. Neuroimmunol. 19:141.
44. Sophianos, D., and S. Tzartos. 1989. Fab fragments of monoclonal antibodies protect the human acetylcholine receptor against antigenic modulation caused by myasthenic sera. J. Autoimmun. 2:777.
45. Conroy, W., M. Saedi, and J. Lindstrom. 1990. TE671 cells express an abundance of a partially mature acetylcholine receptor alpha subunit which has characteristics of an assembly intermediate. J. Biol. Chem. 265:21642.
46. Tomlinson, I., G. Walter, J. Marks. M. Lleweln, and G. Winter. 1992. The repertoire of human germline VH sequences reveals about fifty groups of VH segments with ditferent hypervariable loops. J. Mol. Biol. 227:776.
47. Winkler, T., H. Fehr, and J. Kalden. 1992. Analysis of immunoglobulin variable region genes from human IgG anti-DNA hybridomas. Eur. J. Immunol. 22:1719.
48. Hexham, J. M., J. Furmaniak, C. Pegg, D. R. Burton, and B. Rees Smith. 1992. Cloning of a human autoimmune response: preparation and sequencing of a human anti-thyroglobulin autoantibody using a combinatorial approach. Autoimmunity 12:135.
49. Pascual, V., S. Cha, M. E. Gershwin, J. D. Capra, and P. S. Leung. 1994. Nucleotide sequence analysis of natural and combinatorial anti-PDC-E2 antibodies in patients with primary biliary cirrhosis: recapitulating immune selection with molecular biology. J. Immunol. 152:2577.
50. Barbas, C., III, T. Collet, W. Amberg, P. Roben, J. Binley, D. Hoekstra, D. Cababa, T. Jones, R. Williamson, G. Pilkington, N. Haigwood, A. Satterthwait, I. Sanz, and D. Burton. 1993. Molecular profile of an antibody response to HIV-1 as probed by combinatorial libraries. J. Mol. Biol. 230:812.
51. Ditzel, H. J., J, M. Binley, J. P. Moore, J. Sodroski, N. Sullivan, L. S. Sawyer, R. M. Hendry, W. P. Yang, C. Barbas III, and D. R. Burton. 1995. Neutralizing recombinant human antibodies to a conformational V2-and CD4-binding site-sensitive epitope of HIV-1 gp120 isolated by using an epitope-masking procedure. J. Immunol. 154:893.
52. Drachman, D., C. Angus, R. Adams, J. Michelson, and G. Hofman. 1978. Myasthenic antibodies cross-link acetylcholine receptors to accelerate degradation. N. Engl. J. Med. 298:1116.
53. 2 hybrid molecules. Muscle Nerve 9:306.
54. Mamalaki, A., N. Trakas, and S. J. Tzartos. 1993. Bacterial expression of a single-chain Fv fragment which efficiently protects the acetylcholine receptor against antigenic modulation caused by myasthenic antibodies. Eur. J. Immunol. 23:1839.
55. Yang, W.-P., K. Green, S. Pinz-Sweeney, A. Briones, D. Burton, and C. Barbas III. 1995. CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range. J. Mol. Biol. 254: 392.
56. Burton, D., and J. Woof. 1992. Human antibody effector function. Adv. Immunol. 51:1.