Measurement of key metabolic enzyme activities in mammalian cells using rapid and sensitive microplate-based assays

Biotechnology and Bioengineering

Volumn 107, Issue 3, 2010, Pages 566-581

  Janke, R ^a   Genzel, Y ^a   Wahl, A ^b   Reichl, U ^c  

^a MAX PLANCK INSTITUTE FOR DYNAMICS OF COMPLEX TECHNICAL SYSTEMS   (Germany)

^b DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

^c OTTO VON GUERICKE UNIVERSITY   (Germany)

Author keywords

Enzymatic cycling; Enzyme activities; Glutaminolysis; MDCK; Metabolism

Indexed keywords

ASPARTATES; CELL EXTRACTS; CONSTANT LEVEL; CYCLE SYSTEMS; ENZYMATIC CYCLING; GLUTAMATE DEHYDROGENASE; GLUTAMINASE; GLUTAMINOLYSIS; INFLUENZA VACCINES; LIMIT OF DETECTION; LIMIT OF QUANTITATIONS; LOW LEVEL; MAMMALIAN CELLS; MDCK; MDCK CELLS; MICROPLATES; PRODUCT INHIBITION; RELATIVE STANDARD DEVIATIONS; SENSITIVITY LIMIT; SUBSTRATE CONCENTRATIONS;

AMINO ACIDS; ASSAYS; CATALYSTS; MAMMALS; METABOLISM; ENZYME ACTIVITY; ENZYMES; INTERFERENCE SUPPRESSION; PHYSIOLOGY;

ENZYMES; ASSAYS;

ASPARTATE AMINOTRANSFERASE; GLUTAMATE DEHYDROGENASE; GLUTAMINASE; INFLUENZA VACCINE; CARBON; CELL ENZYME; CELL EXTRACT; GLUTAMATE AMMONIA LIGASE; GLUTAMIC ACID; GLUTAMINE; PYRUVATE CARBOXYLASE; PYRUVATE DEHYDROGENASE;

ANIMAL CELL; ARTICLE; CELL STRAIN; ENZYMATIC ASSAY; ENZYME ACTIVITY; MAMMAL CELL; NONHUMAN; ENZYME ASSAY; ENZYME METABOLISM; LIMIT OF DETECTION; LIMIT OF QUANTITATION; MEASUREMENT; MICROPLATE BASED ASSAY; SENSITIVITY ANALYSIS;

ANIMALS; CARBON; CELL CULTURE TECHNIQUES; CELL EXTRACTS; CELLS, CULTURED; CULTURE MEDIA; DOGS; ENZYMES; GLUTAMINE; HIGH-THROUGHPUT SCREENING ASSAYS; SENSITIVITY AND SPECIFICITY;

MAMMALIA;

EID: 77955664106     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22817     Document Type: Article

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