Optimized spectrophotometric assay for the completely activated pyruvate dehydrogenase complex in fibroblasts

Clinical Chemistry

Volumn 51, Issue 1, 2005, Pages 151-160

  Schwab, Marina A ^a   Kölker, Stefan ^a   Van Den Heuvel, Lambert P ^b,c   Sauer, Sven ^a   Wolf, Nicole I ^a   Rating, Dietz ^a   Hoffmann, Georg F ^a   Smeitink, Jan A M ^b,c   Okun, Jürgen G ^a  

^a UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

^b RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^c NCMD   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

4 IODONITROTETRAZOLIUM VIOLET; CARBON 14; CARBON DIOXIDE; CITRATE SYNTHASE; DITHIOTHREITOL; OXIDOREDUCTASE INHIBITOR; PYRUVATE DEHYDROGENASE COMPLEX; PYRUVIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; TETRAZOLE DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CELL DISRUPTION; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERMETHOD COMPARISON; MALE; MITOCHONDRION; NONHUMAN; PROCESS OPTIMIZATION; PYRUVATE DEHYDROGENASE COMPLEX DEFICIENCY; RADIOCHEMISTRY; RELIABILITY; SKIN FIBROBLAST; SPECTROPHOTOMETRY;

CELLS, CULTURED; DITHIOTHREITOL; FIBROBLASTS; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; MITOCHONDRIA; PYRUVATE DEHYDROGENASE COMPLEX; PYRUVATE DEHYDROGENASE COMPLEX DEFICIENCY DISEASE; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SKIN; SPECTROPHOTOMETRY;

VIOLA (ANGIOSPERM);

EID: 11144258625     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2004.033852     Document Type: Article

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